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Avian reovirus S1133-induced apoptosis is associated with Bip/GRP79-mediated Bim translocation to the endoplasmic reticulum

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Abstract

In this study the mechanism of avian reovirus (ARV) S1133-induced pathogenesis was investigated, with a focus on the contribution of ER stress to apoptosis. Our results showed that upregulation of the ER stress response protein, as well as caspase-3 activation, occurred in ARV S1133-infected cultured cells and in SPF White Leghorn chicks organs. Upon infection, Bim was translocated specifically to the ER, but not mitochondria, in the middle to late infectious stages. In addition, ARV S1133 induced JNK phosphorylation and promoted JNK–Bim complex formation, which correlated with the Bim translocation and apoptosis induction that was observed at the same time point. Knockdown of BiP/GRP78 by siRNA and inhibition of BiP/GRP78 using EGCG both abolished the formation of the JNK–Bim complex, caspase-3 activation, and subsequent apoptosis induction by ARV S1133 efficiently. These results suggest that BiP/GRP78 played critical roles and works upstream of JNK–Bim in response to the ARV S1133-mediated apoptosis process.

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Acknowledgments

We are very grateful to the editors of OxiBiosci Editing Co. for the re-arrangement and re-writing of the manuscript.

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Authors and Affiliations

  1. Department of Biological Science and Technology, National Pingtung University of Science and Technology, 1, Shuefu Rd., Neipu, 91201, Pingtung, Taiwan

    Ping-Yuan Lin, Yo-Chia Chen, Chi-I Chang & Wen-Ling Shih

  2. Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan

    Ching-Dong Chang

  3. Institute of Molecular Biology, National Chung-Hsing University, Taichung, Taiwan

    Hung-Jen Liu

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  1. Ping-Yuan Lin
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Lin, PY., Liu, HJ., Chang, CD. et al. Avian reovirus S1133-induced apoptosis is associated with Bip/GRP79-mediated Bim translocation to the endoplasmic reticulum. Apoptosis 20, 481–490 (2015). https://doi.org/10.1007/s10495-015-1085-5

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