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Mitochondrion-mediated apoptosis induced by Rana grylio virus infection in fish cells

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Abstract

A fish cell line, fathead minnow (FHM) cell, was used to investigate the alteration of mitochondrial dynamics and the mechanism of apoptosis under Rana grylio virus (RGV) infection. Microscopy observations, flow-cytometry analysis and molecular marker detection revealed the apoptotic fate of the RGV-infected cells. Some typical apoptotic characteristics, such as chromatin condensation, DNA fragmentation and mitochondrial fragmentation, were observed, and significantly morphological changes of mitochondria, including size, shape, internal structure and distribution, were revealed. The mitochondria in RGV-infected cells were aggregated around the viromatrix, and the aggregation could be blocked by colchicine. Moreover, the Δψm collapse was induced, and caspase-9 and caspase-3 were activated in the RGV-infected cells. In addition, NF-κB activation and intracellular Ca2+ increase were also detected at different times after infection. The data revealed the detailed dynamics of mitochondrion-mediated apoptosis induced by an iridovirus, and provided the first report on mitochondrial fragmentation during virus-induced apoptosis in fish cells.

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Acknowledgments

Grateful thanks for Ms. Zheng-Qiu Li and Xiu-Ping Yuan who provide great help. This study was supported by grants from the National Major Basic Research Program (2004CB117403), the National 863 High Technology Research Foundation of China (2006AA09Z445 and 2006AA100309), the National Natural Science Foundation of China (30671616 and U0631008) and the Project of Chinese Academy of Sciences (KSCX2-YW-N-021).

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Authors and Affiliations

  1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Wuhan, 430072, China

    You-Hua Huang, Xiao-Hong Huang, Jian-Fang Gui & Qi-Ya Zhang

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  1. You-Hua Huang
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  2. Xiao-Hong Huang
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  3. Jian-Fang Gui
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  4. Qi-Ya Zhang
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Correspondence to Qi-Ya Zhang.

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Huang, YH., Huang, XH., Gui, JF. et al. Mitochondrion-mediated apoptosis induced by Rana grylio virus infection in fish cells. Apoptosis 12, 1569–1577 (2007). https://doi.org/10.1007/s10495-007-0089-1

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