Abstract
The stylostome of larvae of the trombiculids Leptotrombidium scutellare (Nagayo et al.), Leptotrombidium fletcheri (Womersley et Heaslip) and Leptotrombidium deliense (Walch) was studied experimentally at different time intervals after larval attachment using the histological method. The stylostome of these species has the same organization and belongs to the epidermal combined with the mixed type, developing more in width than in length. Neither transverse nor conspicuous longitudinal layers are present within the stylostome walls, which stain predominantly in red with Azan, also showing longitudinal portions with blue staining. Larvae tend to attach closely to each other and scabs, consisting of the hyperkeratotic epidermal layers fusing with migrating inflammatory cells, develop around the attachment sites. The dermis shows inflammatory foci with dilated capillaries and inflammatory cells inserting in the connective tissue layer underneath the stylostome. The feeding cavity, which is moderately expressed, may be found either in the epidermis or in the dermis. It contains inflammatory cells and their debris in the liquefied host tissues. The stylostome length depends on the character of the attachment site (the thicker epidermis or scab the longer the stylostome), and does not directly correspond to the stages of larval feeding. Nevertheless, at the 48-h time interval, nearly all attached larvae are found to be fully fed and their midgut cells are filled with nutritional globules.
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Acknowledgments
The authors gratefully thank to I.E. Borisenko (a graduate student of the St.-Petersburg State University) for his qualified help in histological methods. This study is supported by a grant N 12-04-00354-a from the Russian Foundation for Fundamental Research. Special thanks to anonymous reviewers for their qualified remarks and comments for improvement of this paper.
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Shatrov, A.B., Takahashi, M., Noda, S. et al. Stylostome organization in feeding Leptotrombidium larvae (Acariformes: Trombiculidae). Exp Appl Acarol 64, 33–47 (2014). https://doi.org/10.1007/s10493-014-9809-8
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DOI: https://doi.org/10.1007/s10493-014-9809-8