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Streptomyces carminius sp. nov., a novel actinomycete isolated from Sophora alopecuroides in Xinjiang, China

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Abstract

A novel actinobacterial strain, designated TRM SA0054T, was isolated from the roots of Sophora alopecuroides grown in Alar, Xinjiang, north-west China, and characterised by a polyphasic taxonomic approach. Phylogenetic analysis showed that strain TRM SA0054T has 16S rRNA gene sequence similarity of 98.22% with Streptomyces barkulensis RC 1831T. Whole cell hydrolysates of strain TRM SA0054T were found to contain ll-diaminopimelic acid as the diagnostic diamino acid and ribose, glucose and xylose as the major whole cell sugars. The major fatty acids were identified as iso-C16:0, iso-C16:1 G, anteiso-C17:0, anteiso-C15:0, C16:0, anteiso-C17:1 ω9c. The main menaquinones were determined to be MK-8 (H4), MK-9 (H6) and MK-9 (H8). The polar lipids were identified as diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified lipid. The G + C content of the genomic DNA was determined to be 73.04%. Strain TRM SA0054T has a relatively low DNA–DNA relatedness value with Streptomyces barkulensis RC 1831T as determined by calculating the average nucleotide identity value (ANI = 84.1%). Based on the phenotypic, chemotaxonomic and phylogenetic data, it is concluded that strain TRM SA0054T should be designated as a novel species of the genus Streptomyces, for which the name Streptomyces carminius sp. nov. is proposed, with type strain TRM SA0054T (= CCTCC AA 2016041T = KCTC39903T).

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (Project No. 31560002), and the National Natural Science Foundation of China (Project No. 31500009). Tarim University Graduate Research and Innovation Projects (Project No. TDGRI201718). We thank Aharon Oren, Bernhard Schink and Stefano Ventura who helped with the Latin name proposed.

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  1. Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, College of Life Science, Tarim University, Alar, 843300, People’s Republic of China

    Yang Wang, Zhanfeng Xia, Zhanwen Liu, Chuanxing Wan, Xiaoxia Luo & Lili Zhang

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  1. Yang Wang
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  2. Zhanfeng Xia
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  3. Zhanwen Liu
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Correspondence to Xiaoxia Luo or Lili Zhang.

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Wang, Y., Xia, Z., Liu, Z. et al. Streptomyces carminius sp. nov., a novel actinomycete isolated from Sophora alopecuroides in Xinjiang, China. Antonie van Leeuwenhoek 111, 1807–1814 (2018). https://doi.org/10.1007/s10482-018-1069-x

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