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Sphingorhabdus buctiana sp. nov., isolated from fresh water, and reclassification of Sphingopyxis contaminans as Sphingorhabdus contaminans comb. nov.

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Abstract

A Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterial strain, designated T5T, was isolated from the Chishui River in Maotai town, Guizhou Province, Southwest of China. Strain T5T was found to grow optimally at pH 9.0 and 25 °C. The 16S rRNA gene sequence analysis indicated that strain T5T belongs to the family Sphingomonadaceae within the phylum Proteobacteria; the strain T5T clustered with the type strains of Sphingopyxis contaminans, Sphingorhabdus wooponensis and Sphingorhabdus rigui, with which it exhibits 16S rRNA gene sequence similarity values of 96.2–96.9%. The DNA G+C content was 58.5 mol%. The major respiratory quinone was Q-10 and the major polar lipid was phosphatidylethanolamine. The major polyamine was homospermidine and the major fatty acids were C18:1 ω7c (37.5%) and C16:1 ω7c (30.1%). On the basis of phylogenetic, phenotypic and genetic data, strain T5T represents a novel species of the genus Sphingorhabdus, for which the name Sphingorhabdus buctiana sp. nov. is proposed. The type strain is T5T (= CGMCC 1.12929T = JCM 30114T). It is also proposed that Sphingopyxis contaminans should be reclassified as a member of the genus Sphingorhabdus.

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Acknowledgements

We thank L. Q. Liang and Z. K. Zhang for their help with sample collection.

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Authors and Affiliations

  1. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People’s Republic of China

    Hanyi Chen, Ai-Lian Piao, Han Lu, Qing-Qing Feng & Jie Lv

  2. Food and Health Food R&D Center, Research Institute of Tasly Holding Group Co., LTD., Tianjin, 300410, People’s Republic of China

    Xu Tan

  3. Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka, 237-0061, Japan

    Yuichi Nogi

  4. Beijing NO. 80 Middle School, Beijing, 100102, People’s Republic of China

    Jina Yeo

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  1. Hanyi Chen
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  2. Ai-Lian Piao
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Correspondence to Jie Lv.

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Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain T5T is KJ667149.

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Chen, H., Piao, AL., Tan, X. et al. Sphingorhabdus buctiana sp. nov., isolated from fresh water, and reclassification of Sphingopyxis contaminans as Sphingorhabdus contaminans comb. nov.. Antonie van Leeuwenhoek 111, 323–331 (2018). https://doi.org/10.1007/s10482-017-0954-z

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