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Sphingorhabdus pacificus sp. nov., isolated from sandy sediments of the Sea of Japan seashore

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Abstract

An aerobic, Gram-negative, yellow-pigmented, non-motile rod-shaped bacterium designated KMM 9574T was isolated from a sand sediment sample collected from the Sea of Japan seashore. Comparative 16S rRNA gene sequence analysis showed that strain KMM 9574T belonged to the genus Sphingorhabdus sharing a highest sequence similarity to Sphingorhabdus marina JCM 14161T 96.8 %. Strain KMM 9574T was characterized by the major ubiquinone Q-10, and by the predominance of C18:1 ω7c, C16:0 2-OH, C16:1 ω7c, C17:1, followed by C15:0 2-OH and C14:0 2-OH in its fatty acid profile. Polar lipids consisted of phosphatidylcholine, sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unknown phospholipid, and an unknown lipid. The DNA G+C content was 56.5 mol %. Based on phylogenetic analysis and distinctive phenotypic characteristics, strain 9574T is concluded to represent a novel species of the genus Sphingorhabdus, for which the name Sphingorhabdus pacificus sp. nov., is proposed. The type strain of the species is strain KMM 9574T (= NRIC 0922T = JCM 30177T).

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Acknowledgments

We would like to thank Dr. Moriya Ohkuma, Japan Collection of Microorganisms, JCM, Japan, for providing Sphingorhabdus type strains for comparative analyses. This study was supported by a grant from the RSF “Biodiversity and biotechnological potential of marine bacteria and fungi” no. 14-14-00030.

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Authors and Affiliations

  1. G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, 690022, Vladivostok, Russia

    Lyudmila A. Romanenko & Valery V. Mikhailov

  2. NODAI Culture Collection Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo, 156-8502, Japan

    Naoto Tanaka

  3. Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, 690041, Vladivostok, Russia

    Vassilii I. Svetashev

  4. Far Eastern Federal University, Oktyabr’skaya st., 27, 690091, Vladivostok, Russia

    Valery V. Mikhailov

Authors
  1. Lyudmila A. Romanenko
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  2. Naoto Tanaka
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  3. Vassilii I. Svetashev
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  4. Valery V. Mikhailov
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Corresponding author

Correspondence to Lyudmila A. Romanenko.

Additional information

Communicated by Erko Stackebrandt.

The DDBJ/GenBank/EMBL accession number of the 16S rRNA gene sequence of strain KMM 9574T is AB936074.

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Romanenko, L.A., Tanaka, N., Svetashev, V.I. et al. Sphingorhabdus pacificus sp. nov., isolated from sandy sediments of the Sea of Japan seashore. Arch Microbiol 197, 147–153 (2015). https://doi.org/10.1007/s00203-014-1033-8

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  • DOI: https://doi.org/10.1007/s00203-014-1033-8

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