Abstract
Using a polyphasic taxonomic strategy, an aerobic, Gram-negative, non-motile, yellow pigmented rod isolated from a sputum sample of a patient with pneumonia was characterised. This bacterial strain, designated G972T, could not be identified by our systematic MALDI-TOF screening on a MicroFlex. This led to the sequencing of the 16S rRNA gene, which shows 98.57% sequence identity with that of Chryseobacterium indologenes 16777T, the phylogenetic closely related type strain of a species with standing in nomenclature, which putatively classifies it as a new species. The major cell fatty acids were identified as 13-methyl-tetradecanoic acid (61%), 3-hydroxy-heptadecanoic acid (16%) and 15-methyl-11-hexadecenoic acid (11%). d-glucose, d-mannose, aesculin, d-maltose, d-trehalose, and gentibiose are the main carbon source. Digital DNA–DNA hybridization (dDDH) estimation and average nucleotide identity values (ANI) of the strain G972T against genomes of the type strains of related species ranged between 18.9 and 32.8% and between 71.46 and 83.61%, respectively, thus confirming again the new species status of the strain. Here, we describe the characteristics of this organism, complete genome sequence and annotation. The 5,390,132 bp size genome contains 4867 protein-coding genes, 89 RNAs (three genes are 5S rRNA, one gene is 16S rRNA, one gene is 23S rRNA and 84 tRNAs) with 35.51% GC content. Finally, on the basis of these polyphasic data, consisting of phenotypic and genomic analyses, we conclude that strain strain G972T (= DSM 103388T = CSUR P2233T) represents a novel species for which we propose the name Chryseobacterium timonianum. The 16S rRNA and genome sequences are available in GenBank database under accession numbers LT161886 and FJVD00000000.
Similar content being viewed by others
References
AbdulWahab A, Taj-Aldeen S, Ibrahim EB et al (2015) Discrepancy in MALDI-TOF MS identification of uncommon Gram-negative bacteria from lower respiratory secretions in patients with cystic fibrosis. IDR 8:83–86. doi:10.2147/IDR.S80341
Auch AF, von Jan M, Klenk H-P, Göker M (2010) Digital DNA–DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genom Sci 2:117–134. doi:10.4056/sigs.531120
Aziz RK, Bartels D, Best AA et al (2008) The RAST server: rapid annotations using subsystems technology. BMC Genom 9:75. doi:10.1186/1471-2164-9-75
Behrendt U, Ulrich A, Sproer C, Schumann P (2007) Chryseobacterium luteum sp. nov., associated with the phyllosphere of grasses. Int J Syst Evol Microbiol 57:1881–1885. doi:10.1099/ijs.0.65104-0
Ben Salah I, Fekih Hassen M, Ben Haj Khalifa A et al (2015) Pneumopathie à Chryseobacterium indologenes acquise sous ventilation mécanique: premier cas rapporté en Tunisie. Rev Pneumol Clin 71:374–375. doi:10.1016/j.pneumo.2015.04.001
Benmalek Y, Cayol J-L, Bouanane NA et al (2010) Chryseobacterium solincola sp. nov., isolated from soil. Int J Syst Evol Microbiol 60:1876–1880. doi:10.1099/ijs.0.008631-0
Bittar F, Keita MB, Lagier J-C et al (2014) Gorilla gorilla gorilla gut: a potential reservoir of pathogenic bacteria as revealed using culturomics and molecular tools. Sci Rep 4:7174. doi:10.1038/srep07174
Cimmino T, Rolain JM (2016) Whole genome sequencing for deciphering the resistome of Chryseobacterium indologenes, an emerging multidrug-resistant bacterium isolated from a cystic fibrosis patient in Marseille, France. New Microbes New Infect 12:35–42. doi:10.1016/j.nmni.2016.03.006
De Beer H, Hugo CJ, Jooste PJ et al (2005) Chryseobacterium vrystaatense sp. nov., isolated from raw chicken in a chicken-processing plant. Int J Syst Evol Microbiol 55:2149–2153. doi:10.1099/ijs.0.63746-0
Dione N, Sankar SA, Lagier JC et al (2016) Genome sequence and description of Anaerosalibacter massiliensis sp. nov. New Microbes New Infect 10:66–76. doi:10.1016/j.nmni.2016.01.002
Drissi F, Buffet S, Raoult D, Merhej V (2015) Common occurrence of antibacterial agents in human intestinal microbiota. Front Microbiol 6:441. doi:10.3389/fmicb.2015.00441
Gallego V, García MT, Ventosa A (2006) Chryseobacterium hispanicum sp. nov., isolated from the drinking water distribution system of Sevilla, Spain. Int J Syst Evol Microbiol 56:1589–1592. doi:10.1099/ijs.0.64264-0
Gupta SK, Padmanabhan BR, Diene SM et al (2014) ARG-ANNOT, a new bioinformatic tool to discover antibiotic resistance genes in bacterial genomes. Antimicrob Agents Chemother 58:212–220. doi:10.1128/AAC.01310-13
Hadjadj L, Rathored J, Keita MB et al (2016) Non contiguous-finished genome sequence and description of Microbacterium gorillae sp. nov. Stand Genom Sci 11:32. doi:10.1186/s40793-016-0152-z
Hantsis-Zacharov E, Halpern M (2007) Chryseobacterium haifense sp. nov., a psychrotolerant bacterium isolated from raw milk. Int J Syst Evol Microbiol 57:2344–2348. doi:10.1099/ijs.0.65115-0
Hantsis-Zacharov E, Shaked T, Senderovich Y, Halpern M (2008) Chryseobacterium oranimense sp. nov., a psychrotolerant, proteolytic and lipolytic bacterium isolated from raw cow's milk. Int J Syst Evol Microbiol 58:2635–2639. doi:10.1099/ijs.0.65819-0
Hernandez-Divers SJ, Hensel P, Gladden J et al (2009) Investigation of shell disease in map turtles (Graptemys spp.). J Wildl Dis 45:637–652. doi:10.7589/0090-3558-45.3.637
Hsueh PR, Hsiue TR, Wu JJ et al (1996) Flavobacterium indologenes bacteremia: clinical and microbiological characteristics. Clin Infect Dis 23:550–555
Kämpfer P, Poppel MT, Wilharm G et al (2014) Chryseobacterium gallinarum sp nov., isolated from a chicken, and Chryseobacterium contaminans sp nov., isolated as a contaminant from a rhizosphere sample. Int J Syst Evol Microbiol 64:1419–1427. doi:10.1099/ijs.0.058933-0
Kim T, Kim M, Kang O et al (2015) Chryseobacterium frigidum sp. nov., isolated from high Arctic tundra soil, and emended description of Chryseobacterium bernardetii and Chryseobacterium taklimakanense. Int J Syst Evol Microbiol 66:609–615. doi:10.1099/ijsem.0.000761
Lagier JC, Armougom F, Million M et al (2012) Microbial culturomics: paradigm shift in the human gut microbiome study. Clin Microbiol Infect 18:1185–1193. doi:10.1111/1469-0691.12023
Lijnen HR, Van Hoef B, Ugwu F et al (2000) Specific Proteolysis of Human Plasminogen by a 24 kDa Endopeptidase from a Novel Chryseobacterium sp. Biochemistry 39:479–488. doi:10.1021/bi992014r
Lin Y-T, Jeng Y-Y, Lin M-L et al (2010) Clinical and microbiological characteristics of Chryseobacterium indologenes bacteremia. J Microbiol Immunol Infect 43:498–505. doi:10.1016/S1684-1182(10)60077-1
McKew G (2014) Severe sepsis due to Chryseobacterium indologenes in an immunocompetent adventure traveler. J Clin Microbiol 52:4100–4101. doi:10.1128/JCM.01691-14
Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M (2013) Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinform 14:60. doi:10.1186/1471-2105-14-60
Montero-Calasanz MDC, Goker M, Rohde M et al (2013) Chryseobacterium hispalense sp. nov., a plant-growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery, and emended descriptions of Chryseobacterium defluvii, Chryseobacterium indologenes, Chryseobacterium wanjuense and Chryseobacterium gregarium. Int J Syst Evol Microbiol 63:4386–4395. doi:10.1099/ijs.0.052456-0
Park MS, Jung SR, Lee KH et al (2006) Chryseobacterium soldanellicola sp. nov. and Chryseobacterium taeanense sp. nov., isolated from roots of sand-dune plants. Int J Syst Evol Microbiol 56:433–438. doi:10.1099/ijs.0.63825-0
Ramasamy D, Mishra AK, Lagier J-C et al (2014) A polyphasic strategy incorporating genomic data for the taxonomic description of novel bacterial species. Int J Syst Evol Microbiol 64:384–391. doi:10.1099/ijs.0.057091-0
Riffel A, Lucas F, Heeb P, Brandelli A (2003) Characterization of a new keratinolytic bacterium that completely degrades native feather keratin. Arch Microbiol 179:258–265. doi:10.1007/s00203-003-0525-8
Rosselló-Móra R, Trujillo ME, Sutcliffe IC (2017) Introducing a digital protologue: a timely move towards a database-driven systematics of Archaea and Bacteria. Syst Appl Microbiol 40:121–122. doi:10.1016/j.syapm.2017.02.001
Sasser M (2016) Bacterial identiffication by gas chromatographic analysis of fatty acids methyl esters (GC-FAME) TechNote_101. MIDI Inc, Newark
Sharma P, Gupta SK, Diene SM, Rolain JM (2015) Whole-genome sequence of Chryseobacterium oranimense, a colistin-resistant bacterium isolated from a cystic fibrosis patient in France. Antimicrob Agents Chemother 59:1696–1706. doi:10.1128/AAC.02417-14
Shimomura K, Kaji S, Hiraishi A (2005) Chryseobacterium shigense sp. nov., a yellow-pigmented, aerobic bacterium isolated from a lactic acid beverage. Int J Syst Evol Microbiol 55:1903–1906. doi:10.1099/ijs.0.63690-0
Singh P, Kim Y-J, Farh ME-A et al (2016) Chryseobacterium panacis sp. nov., isolated from ginseng soil. Antonie Van Leeuwenhoek 109:187–196. doi:10.1007/s10482-015-0620-2
Tai CJ, Kuo HP, Lee FL et al (2006) Chryseobacterium taiwanense sp. nov., isolated from soil in Taiwan. Int J Syst Evol Microbiol 56:1771–1776. doi:10.1099/ijs.0.64294-0
Tamura K, Peterson D, Peterson N et al (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739. doi:10.1093/molbev/msr121
Vandamme P, Bernadet JF, Segers P et al (1994) NOTES: new perspectives in the classification of the flavobacteria: description of Chryseobacterium gen. nov., Bergeyella gen. nov., and Empedobacter nom. rev. Int J Syst Bacteriol 44:827–831. doi:10.1099/00207713-44-4-827
Venil CK, Nordin N, Zakaria ZA, Ahmad WA (2014) Chryseobacterium artocarpi sp. nov., isolated from the rhizosphere soil of Artocarpus integer. Int J Syst Evol Microbiol 64:3153–3159. doi:10.1099/ijs.0.063594-0
Wang X, Hu Z, Fan Y, Wang H (2013) Chryseobacterium indologenes catheter-related meningitis in an elderly patient after intracranial aneurysm clipping surgery. Neurol Sci 35:113–115. doi:10.1007/s10072-013-1500-z
Weber T, Blin K, Duddela S et al (2015) antiSMASH 3.0-a comprehensive resource for the genome mining of biosynthetic gene clusters. Nucleic Acids Res 43:W237–W243. doi:10.1093/nar/gkv437
Xu L, Huo Y-Y, Li Z-Y et al (2015) Chryseobacterium profundimaris sp. nov., a new member of the family Flavobacteriaceae isolated from deep-sea sediment. Antonie Van Leeuwenhoek 107:979–989. doi:10.1007/s10482-015-0390-x
Young CC, Kämpfer P, Shen FT et al (2005) Chryseobacterium formosense sp. nov., isolated from the rhizosphere of Lactuca sativa L. (garden lettuce). Int J Syst Evol Microbiol 55:423–426. doi:10.1099/ijs.0.63331-0
Acknowledgements
The authors thank the Xegen Company (www.xegen.fr) for automating the genomic annotation process and Karolina Griffiths for English reviewing.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
We have no conflicts of interest to declare.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Abou Abdallah, R., Cimmino, T., Baron, S. et al. Description of Chryseobacterium timonianum sp. nov., isolated from a patient with pneumonia. Antonie van Leeuwenhoek 110, 1121–1132 (2017). https://doi.org/10.1007/s10482-017-0885-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10482-017-0885-8