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Biofilm formation and fibrinogen and fibronectin binding activities by Corynebacterium pseudodiphtheriticum invasive strains

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Abstract

Biofilm-related infections are considered a major cause of morbidity and mortality in hospital environments. Biofilms allow microorganisms to exchange genetic material and to become persistent colonizers and/or multiresistant to antibiotics. Corynebacterium pseudodiphtheriticum (CPS), a commensal bacterium that colonizes skin and mucosal sites has become progressively multiresistant and responsible for severe nosocomial infections. However, virulence factors of this emergent pathogen remain unclear. Herein, we report the adhesive properties and biofilm formation on hydrophilic (glass) and hydrophobic (plastic) abiotic surfaces by CPS strains isolated from patients with localized (ATCC10700/Pharyngitis) and systemic (HHC1507/Bacteremia) infections. Adherence to polystyrene attributed to hydrophobic interactions between bacterial cells and this negatively charged surface indicated the involvement of cell surface hydrophobicity in the initial stage of biofilm formation. Attached microorganisms multiplied and formed microcolonies that accumulated as multilayered cell clusters, a step that involved intercellular adhesion and synthesis of extracellular matrix molecules. Further growth led to the formation of dense bacterial aggregates embedded in the exopolymeric matrix surrounded by voids, typical of mature biofilms. Data also showed CPS recognizing human fibrinogen (Fbg) and fibronectin (Fn) and involvement of these sera components in formation of “conditioning films”. These findings suggested that biofilm formation may be associated with the expression of different adhesins. CPS may form biofilms in vivo possibly by an adherent biofilm mode of growth in vitro currently demonstrated on hydrophilic and hydrophobic abiotic surfaces. The affinity to Fbg and Fn and the biofilm-forming ability may contribute to the establishment and dissemination of infection caused by CPS.

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Acknowledgments

This work was supported by grant from CAPES, CNPq, FAPERJ, SR-2/UERJ, PNPD (CNPq/CAPES), PAPD (FAPERJ/CAPES) and PRONEX of the Brazilian Ministry of Science and Technology. We are also grateful to Noêmia Rodrigues Gonçalves Alves from Cellular Ultrastructure Laboratory Hertha Meyer-Universidade Federal do Rio de Janeiro, for electron microscopy technical assistance.

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    Authors and Affiliations

    1. Laboratório de Difteria e Corinebactérias de Importância Clínica (LDCIC), Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro (UERJ), Av. 28 de Setembro, 87 - Fundos, 3°andar, Vila Isabel, Rio de Janeiro, RJ, 20.551-030, Brazil

      Monica Cristina Souza, Louisy Sanches dos Santos, Yuri Vieira Faria, Cíntia Silva da Santos, Raphael Hirata Júnior & Ana Luiza Mattos-Guaraldi

    2. Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro (UERJ), Rio de Janeiro, RJ, Brazil

      Prescilla Emy Nagao

    3. Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil

      Leonardo Paiva Sousa, Juliana Nunes Ramos & Verônica Viana Vieira

    4. Faculdade de Farmácia, Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro (IFRJ), Rio de Janeiro, RJ, Brazil

      Débora Leandro Rama Gomes

    5. Laboratório de Doenças Bacterianas Respiratórias e Sistêmicas, Universidade Ceuma (UniCEUMA), São Luís, MA, Brazil

      Priscila Soares Sabbadini

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    1. Monica Cristina Souza
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    Correspondence to Ana Luiza Mattos-Guaraldi.

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    Monica Cristina Souza and Louisy Sanches dos Santos have contributed equally to this work.

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    Souza, M.C., dos Santos, L.S., Sousa, L.P. et al. Biofilm formation and fibrinogen and fibronectin binding activities by Corynebacterium pseudodiphtheriticum invasive strains. Antonie van Leeuwenhoek 107, 1387–1399 (2015). https://doi.org/10.1007/s10482-015-0433-3

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