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Streptomyces alkaliphilus sp. nov., isolated from sediments of Lake Elmenteita in the Kenyan Rift Valley

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Abstract

A novel strain, designated No. 7T, was isolated from a sediment sample collected from the alkaline, saline Lake Elmenteita located in the Kenyan Rift Valley. The optimal growth for the strain was found to be at temperature 30–35 °C, at pH 8.0–12.0 in the presence of 7.0–10.0 % (w/v) NaCl. The strain was observed to form a light green beige abundant aerial mycelium on Horikoshi 1 agar and to have morphological and chemotaxonomic characteristics typical of members of the genus Streptomyces. The peptidoglycan was found to contain LL-diaminopimelic acid as the diamino acid, with no diagnostic sugars identified. The predominant menaquinone was identified as MK-9(H6). The main polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unknown phospholipid. Cellular fatty acids were found to consist of saturated branched-chain acids with iso-C15:0, anteiso-C15:0, iso-C16:0 and anteiso-C17:0 acids predominating. The type strain had a genomic DNA G+C content of 72.8 mol% and formed a distinct phyletic line within the genus Streptomyces. Based on the chemotaxonomic results, 16S rRNA gene sequence analysis and the low DNA–DNA hybridization value with the type strain of Streptomyces calidiresistens, it is proposed that strain No. 7T (= DSM 42118 = CECT 8549) represents a novel species, Streptomyces alkaliphilus. The INSDC accession number for the 16S rRNA gene sequence of strain No. 7T is KF976730.

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Acknowledgments

We would like to gratefully acknowledge the guidance and assistance of Gabriele Pötter and Brian J. Tindall for chemotaxonomic analyses (both DSMZ, Braunschweig). This work was supported by DAAD within a Ph. D. Scholarship (Sandwich model) for J.K. Akhwale. The work was done at the DSMZ (German Collection of Microorganisms and Cell Cultures) Braunschweig. Research authorization in Kenya was given by the National Commission for Science, Technology and Innovation (NACOSTI), Kenya Wildlife Service (KWS) and National Environmental Management Authority (NEMA).

Conflict of interest

Authors disclose that there are no conflicts of interest. No research involving human participants and/or animals was performed. No non-financial interests tied directly or indirectly to this research exist that may be important to readers to be disclosed.

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Authors and Affiliations

  1. Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Brunswick, Germany

    Juliah Khayeli Akhwale, Markus Göker, Cathrin Spröer, Peter Schumann & Hans-Peter Klenk

  2. School of Biology, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK

    Hans-Peter Klenk

  3. Institute for Biotechnology Research, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya

    Juliah Khayeli Akhwale

  4. Taita Taveta University College, P.O. Box 635-80300, Voi, Kenya

    Hamadi Iddi Boga

  5. HZI – Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124, Brunswick, Germany

    Manfred Rohde

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  1. Juliah Khayeli Akhwale
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Correspondence to Hans-Peter Klenk.

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10482_2015_418_MOESM1_ESM.tif

Supplementary Figure 1. Polar lipids profile of Streptomyces alkaliphilus sp. nov. No.7T, after separation by two-dimensional TLC. Plate was sprayed with molybdophosphoric acid for detection of total polar lipid. DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PI, phosphatidylinositol and PL, unknown phospholipid

10482_2015_418_MOESM2_ESM.pdf

Supplementary Figure 2. Heatmaps generated using the lipid extensions of the opm package (Vaas et al. 2013), showing un-transformed and log-transformed measurements respectively, under default settings after importing the result files from the MIDI system. Strains: S. alkaliphilus sp. nov. No. 7T DSM 42118, S. qinglanensis DSM 42035T, S. marinus DSM 41970T, S. tateyamensis DSM 41969T, S. hebeiensis DSM 41837T, S. erringtonii DSM 42088T, S. glauciniger DSM 41867T and S. calidiresistens DSM 42108T

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Akhwale, J.K., Göker, M., Rohde, M. et al. Streptomyces alkaliphilus sp. nov., isolated from sediments of Lake Elmenteita in the Kenyan Rift Valley. Antonie van Leeuwenhoek 107, 1249–1259 (2015). https://doi.org/10.1007/s10482-015-0418-2

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