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Oenococcus alcoholitolerans sp. nov., a lactic acid bacteria isolated from cachaça and ethanol fermentation processes

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Abstract

Four strains of lactic acid bacteria isolated from cachaça and alcohol fermentation vats in Brazil were characterised in order to determine their taxonomic position. Phylogenetic analysis revealed that they belong to the genus Oenococcus and should be distinguished from their closest neighbours. The 16S rRNA gene sequence similarity against the type strains of the other two species of the genus was below 94.76 % (Oenococcus kitaharae) and 94.62 % (Oenococcus oeni). The phylogeny based on pheS gene sequences also confirmed the position of the new taxon. DNA–DNA hybridizations based on in silico genome-to-genome comparison, Average Amino Acid Identity, Average Nucleotide Identity and Karlin genomic signature confirmed the novelty of the taxon. Distinctive phenotypic characteristics are the ability to metabolise sucrose but not trehalose. The name Oenococcus alcoholitolerans sp. nov. is proposed for this taxon, with the type strain UFRJ-M7.2.18T ( = CBAS474T = LMG27599T). In addition, we have determined a draft genome sequence of the type strain.

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Acknowledgments

Financial support came from Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), Fundação de Amparo a Pesquisa do Estado de Pernambuco (FACEPE), Fundação de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Authors and Affiliations

  1. Departamento de Microbiologia, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais, C.P. 486, Belo Horizonte, MG, 31270-901, Brazil

    Fernanda Badotti & Carlos A. Rosa

  2. Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-599, Brazil

    Ana Paula B. Moreira, Luciane A. Chimetto Tonon & Cristiane C. Thompson

  3. Centro de Ciências Biológicas e Sociais Aplicadas, Universidade Estadual da Paraíba, João Pessoa, Brazil

    Brígida T. Luckwu de Lucena

  4. Departamento de Química, Centro Federal de Educação Tecnológica de Minas Gerais, Belo Horizonte, Brazil

    Fátima de Cássia O. Gomes

  5. Laboratório de Enzimologia, Departamento de Biologia Celular, Instituto de Biologia, Universidade de Brasília, Brasília, Brazil

    Ricardo Kruger

  6. Departamento de Genética, Universidade Federal de Pernambuco, Recife, Brazil

    Marcos Antonio de Morais Jr.

  7. Instituto de Biologia and Laboratório de Sistemas Avançados de Gestão da Produção - SAGE/ COPPE, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-972, Brazil

    Fabiano L. Thompson

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  1. Fernanda Badotti
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  2. Ana Paula B. Moreira
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  3. Luciane A. Chimetto Tonon
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  8. Marcos Antonio de Morais Jr.
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Correspondence to Fernanda Badotti.

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Fernanda Badotti and Ana Paula B. Moreira have contributed equally to this work.

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Badotti, F., Moreira, A.P.B., Tonon, L.A.C. et al. Oenococcus alcoholitolerans sp. nov., a lactic acid bacteria isolated from cachaça and ethanol fermentation processes. Antonie van Leeuwenhoek 106, 1259–1267 (2014). https://doi.org/10.1007/s10482-014-0296-z

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