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Micromonospora avicenniae sp. nov., isolated from a root of Avicennia marina

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Abstract

Strain 268506T was isolated from a root of Avicennia marina collected at mangrove forest in Wengchang, Hainan province, China. The 16S rRNA gene sequence of strain 268506T showed the highest similarity with Micromonospora equina Y22T (98.8 %) and Micromonospora olivasterospora DSM 43868T (98.7 %). In addition, gyrB gene phylogeny clearly showed strain 268506T should be assigned to the genus Micromonospora but different from any established Micromonospora species. The predominant menaquinones are MK-9(H8) and MK-9(H6). The major fatty acids are iso-C16:0, iso-C15:0 and anteiso-C17:0. The characteristic whole-cell sugars are xylose, mannose and arabinose. The cell wall contains meso-DAP and glycine. Phosphatidylinositol, diphosphatidylglycerol and phosphatidylethanolamine are the characteristic polar lipids. The DNA G+C content is 70.3 mol%. Some physiological and biochemical properties combined with low DNA–DNA relatedness indicated that the novel strain could be readily distinguished from the closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, strain 268506T represents a novel species of the genus Micromonospora, for which the name Micromonospora avicenniae sp. nov. is proposed. The type strain is 268506T ( = CCTCC AA 2012010T = DSM 45758T).

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Acknowledgments

This research was supported by grants from the National Natural Science Foundation of China (No. 31170467). The authors are grateful to Ina Franc and Hans-Peter Klenk (both DSMZ) for sending us the reference strain, which made the identification completed timely.

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Authors and Affiliations

  1. College of Agronomy, Hainan University, Haikou, 570228, People’s Republic of China

    Lei Li & Kui Hong

  2. Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, People’s Republic of China

    Lei Li, You-Ju Mao, Zixin Deng & Kui Hong

  3. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, 571101, People’s Republic of China

    Lei Li, Qing-Yi Xie & Kui Hong

Authors
  1. Lei Li
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  2. You-Ju Mao
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  3. Qing-Yi Xie
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  4. Zixin Deng
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  5. Kui Hong
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Corresponding author

Correspondence to Kui Hong.

Additional information

The GenBank accession number for the 16S rRNA gene and gyrB gene sequences of Micromonospora avicenniae 268506T are JQ867183 and JQ867184, respectively; and the gyrB gene sequence of Micromonospora equina Y22T is JX885646.

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Li, L., Mao, YJ., Xie, QY. et al. Micromonospora avicenniae sp. nov., isolated from a root of Avicennia marina . Antonie van Leeuwenhoek 103, 1089–1096 (2013). https://doi.org/10.1007/s10482-013-9888-2

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