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Micromonospora azadirachtae sp. nov., isolated from roots of Azadirachta indica A. Juss. var. siamensis Valeton

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Abstract

A Gram-stain positive actinomycete, strain AZ1-19T, isolated from roots of Azadirachta indica A. Juss. var. siamensis Valeton, collected from Chachoengsao province, Thailand, was characterised taxonomically by using a polyphasic approach. Strain AZ1-19T was found to have characteristics consistent with those of members of the genus Micromonospora. The cell wall peptidoglycan of the strain was found to contain meso-diaminopimelic acid. The predominant phospholipids were identified as diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The characteristic whole-cell sugars were identified as glucose, xylose, galactose and mannose. The major menaquinones were found to be MK-9(H6), MK-10(H6) and MK-10(H8), and the major cellular fatty acids were identified as iso-C16:0, iso-C15:0, anteiso-C15:0 and anteiso-C17:0. Comparative analysis of 16S rRNA gene sequences revealed that strain AZ1-19T is closely related to Micromonospora costi CS1-12T (98.75% similarity), Micromonospora avicenniae 268506T (98.75%), Micromonospora haikouensis 232617T (98.68%) and Micromonospora siamensis TT2-4T (98.61%), whilst the corresponding phylogenetic analysis based on partial gyrase subunit B (gyrB) gene sequences indicated that strain AZ1-19T forms a clade with M. avicenniae 268506T with a high bootstrap value. The DNA G + C content was determined to be 69.8 mol%. Moreover, a combination of DNA–DNA relatedness values and some phenotypic and chemotaxonomic properties indicated that the strain could be distinguished from closely related species. Therefore, it is considered that strain AZ1-19T represents a novel Micromonospora species for which the name Micromonospora azadirachtae sp. nov. is proposed. The type strain is AZ1-19T (= KCTC 39941T = NBRC 112784T = JCM 32148T = TISTR 2559T).

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Acknowledgements

We would like to thank Dr A. Matsumoto and Prof Dr Y. Takahashi, Department of Drug Discovery, Kitasato University for the isoprenoid quinone analysis by LC-MS, and appreciate Assoc Prof Dr C. Thawai, Department of Biology, Faculty of Science, King’s Mongkut Institute of Technology Ladkrabang, for providing M. costi CS1-12T. We also thank the 2015 Royal Golden Jubilee Ph. D. Programme scholarship to N. K. for financial support under the Thailand Research Fund.

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  1. Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, 254 Phayathai Road, Wangmai, Bangkok, 10330, Thailand

    Nattakorn Kuncharoen & Somboon Tanasupawat

  2. Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074, Japan

    Takuji Kudo & Moriya Ohkuma

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  1. Nattakorn Kuncharoen
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  2. Takuji Kudo
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We declare that the present study was performed by the authors named in this article. N. Kuncharoen, S. Tanasupawat designed the study; N. Kuncharoen, S. Tanasupawat, and T. Kudo performed experiments on isolation, identification, analysis, and interpretation; M. Ohkuma gave technical support and conceptual advice. All authors read and approved the final manuscript.

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Correspondence to Somboon Tanasupawat.

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Kuncharoen, N., Kudo, T., Ohkuma, M. et al. Micromonospora azadirachtae sp. nov., isolated from roots of Azadirachta indica A. Juss. var. siamensis Valeton. Antonie van Leeuwenhoek 112, 253–262 (2019). https://doi.org/10.1007/s10482-018-1152-3

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