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Ruegeria meonggei sp. nov., an alphaproteobacterium isolated from ascidian Halocynthia roretzi

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Abstract

A Gram-negative, strictly aerobic, non-flagellated and rod-shaped bacterial strain, designated MA-E2-3T, was isolated from an ascidian (Halocynthia roretzi) collected from the South Sea, South Korea. Strain MA-E2-3T was found to grow optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain MA-E2-3T fell within the clade comprising Ruegeria species, clustering consistently with the type strain of Ruegeria halocynthiae, with which it exhibited 98.2 % sequence similarity. Sequence similarities to the type strains of the other recognized Ruegeria species were 94.7–97.7 %. Strain MA-E2-3T was found to contain Q-10 as the predominant ubiquinone and C18:1 ω7c as the predominant fatty acid. The major polar lipids of strain MA-E2-3T were identified as phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain MA-E2-3T was determined to be 58.0 mol%. Mean DNA–DNA relatedness values between strain MA-E2-3T and the type strains of four phylogenetically closely related Ruegeria species were in the range of 13–23 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain MA-E2-3T is separated from other Ruegeria species. On the basis of the data presented, strain MA-E2-3T (=KCTC 32450T = CECT 8411T) represents a novel species of the genus Ruegeria, for which the name Ruegeria meonggei sp. nov. is proposed.

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References

  • Barrow GI, Feltham RKA (1993) Cowan and Steel’s manual for the identification of medical bacteria, 3rd edn. Cambridge University Press, Cambridge

    Book  Google Scholar 

  • Baumann P, Baumann L (1981) The marine gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. In: Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG (eds) The prokaryotes. Springer, Berlin, pp 1302–1331

    Google Scholar 

  • Bruns A, Rohde M, Berthe-Corti L (2001) Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006

    Article  CAS  PubMed  Google Scholar 

  • Cohen-Bazire G, Sistrom WR, Stanier RY (1957) Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J Cell Comp Physiol 49:25–68

    Article  CAS  Google Scholar 

  • Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229

    Article  Google Scholar 

  • Huo YY, Xu XW, Li X, Liu C, Cui HL, Wang CS, Wu M (2011) Ruegeria marina sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 61:347–350

    Article  CAS  PubMed  Google Scholar 

  • Kämpfer P, Arun AB, Rekha PD, Busse HJ, Young CC, Glaeser SP (2013) Ruegeria intermedia sp. nov., a moderately thermophilic bacterium isolated from a coastal hot spring. Int J Syst Evol Microbiol 63:2538–2544

    Article  PubMed  Google Scholar 

  • Kim YO, Park S, Nam BH, Kang SJ, Hur YB, Lee SJ, Oh TK, Yoon JH (2012a) Ruegeria halocynthiae sp. nov., isolated from the sea squirt Halocynthia roretzi. Int J Syst Evol Microbiol 62:925–930

    Article  CAS  PubMed  Google Scholar 

  • Kim YO, Park S, Nam BH, Kang SJ, Hur YB, Kim DG, Oh TK, Yoon JH (2012b) Description of Litoreibacter meonggei sp. nov., isolated from the sea squirt Halocynthia roretzi, reclassification of Thalassobacter arenae as Litoreibacter arenae comb. nov. and emended description of the genus Litoreibacter Romanenko et al. 2011. Int J Syst Evol Microbiol 62:1825–1831

    Article  CAS  PubMed  Google Scholar 

  • Kim YO, Park S, Nam BH, Jung YT, Kim DG, Lee JS, Lee SJ, Yoon JH (2013) Litoreibacter halocynthiae sp. nov., isolated from the sea squirt Halocynthia roretzi. Int J Syst Evol Microbiol 63:3364–3368

    Article  CAS  PubMed  Google Scholar 

  • Komagata K, Suzuki KI (1987) Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–207

    Article  CAS  Google Scholar 

  • Lányí B (1987) Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67

    Article  Google Scholar 

  • Lee J, Whon TW, Shin NR, Roh SW, Kim J, Park SK, Kim MS, Shin KS, Lee JS, Lee KC, Kim YO, Bae JW (2012) Ruegeria conchae sp. nov., isolated from the ark clam Scapharca broughtonii. Int J Syst Evol Microbiol 62:2851–2857

    Article  CAS  PubMed  Google Scholar 

  • Leifson E (1963) Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184

    CAS  PubMed Central  PubMed  Google Scholar 

  • Minnikin DE, Patel PV, Alshamaony L, Goodfellow M (1977) Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117

    Article  CAS  Google Scholar 

  • Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241

    Article  CAS  Google Scholar 

  • Muramatsu Y, Uchino Y, Kasai H, Suzuki K, Nakagawa Y (2007) Ruegeria mobilis sp. nov., a member of the Alphaproteobacteria isolated in Japan and Palau. Int J Syst Evol Microbiol 57:1304–1309

    Article  CAS  PubMed  Google Scholar 

  • Oh KH, Jung YT, Oh TK, Yoon JH (2011) Ruegeria faecimaris sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 61:1182–1188

    Article  CAS  PubMed  Google Scholar 

  • Park S, Yoon JH (2012) Ruegeria arenilitoris sp. nov., isolated from the seashore sand around a seaweed farm. Antonie Van Leeuwenhoek 102:581–589

    Article  PubMed  Google Scholar 

  • Park S, Yoon JH (2013) Ruegeria arenilitoris sp. nov. In list of new names and new combinations previously effectively, but not validly, published, validation list no. 149. Int J Syst Evol Microbiol 63:1–5

    Article  Google Scholar 

  • Petursdottir SK, Kristjansson JK (1997) Silicibacter lacuscaerulensis gen. nov., sp. nov., a mesophilic moderately halophilic bacterium characteristic of the Blue Lagoon geothermal lake in Iceland. Extremophiles 1:94–99

    Article  CAS  PubMed  Google Scholar 

  • Rüger HJ, Höfle MG (1992) Marine star-shaped-aggregate-forming bacteria: Agrobacterium atlanticum sp. nov.; Agrobacterium meteori sp. nov.; Agrobacterium ferrugineum sp. nov., nom. rev.; Agrobacterium gelatinovorum sp. nov., nom. rev.; and Agrobacterium stellulatum sp. nov., nom. rev. Int J Syst Bacteriol 42:133–143

    Article  PubMed  Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI technical note 101. Microbial ID, Inc., Newark

  • Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849

    Article  CAS  Google Scholar 

  • Staley JT (1968) Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942

    CAS  PubMed Central  PubMed  Google Scholar 

  • Tamaoka J, Komagata K (1984) Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128

    Article  CAS  Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl Acids Res 22:4673–4680

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Uchino Y, Hirata A, Yokota A, Sugiyama J (1998) Reclassification of marine Agrobacterium species: proposals of Stappia stellulata gen. nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov., Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev. J Gen Appl Microbiol 44:201–210

    Article  CAS  PubMed  Google Scholar 

  • Vandecandelaere I, Nercessian O, Segaert E, Achouak W, Faimali M, Vandamme P (2008) Ruegeria scottomollicae sp. nov., isolated from a marine electroactive biofilm. Int J Syst Evol Microbiol 58:2726–2733

    Article  CAS  PubMed  Google Scholar 

  • Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE et al (1987) International committee on systematic bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464

    Article  Google Scholar 

  • Yi H, Lim YW, Chun J (2007) Taxonomic evaluation of the genera Ruegeria and Silicibacter: a proposal to transfer the genus Silicibacter Petursdottir and Kristjansson 1999 to the genus Ruegeria Uchino et al. 1999. Int J Syst Evol Microbiol 57:815–819

    Article  CAS  PubMed  Google Scholar 

  • Yoon JH, Kim H, Kim SB, Kim HJ, Kim WY, Lee ST, Goodfellow M, Park YH (1996) Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505

    Article  CAS  Google Scholar 

  • Yoon JH, Lee ST, Park YH (1998) Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194

    Article  CAS  PubMed  Google Scholar 

  • Yoon JH, Kang KH, Park YH (2003) Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454

    Article  CAS  PubMed  Google Scholar 

  • Yoon JH, Kang SJ, Lee SY (2012) Salinimonas lutimais sp. nov., a polysaccharide-degrading bacterium isolated from a tidal flat. Antonie Van Leeuwenhoek 101:803–810

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by grants from the National Fisheries Research and Development Institute (NFRDI) and Program for Collection, Management and Utilization of Biological Resources from the Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea. We are grateful to Prof. Jin-Woo Bae for kindly providing Ruegeria conchae TW15T.

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Authors and Affiliations

  1. Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Gijang, Busan, 619-705, South Korea

    Young-Ok Kim, Bo-Hye Nam & Dong-Gyun Kim

  2. Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea

    Sooyeon Park, Yong-Taek Jung & Jung-Hoon Yoon

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  1. Young-Ok Kim
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  2. Sooyeon Park
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  3. Bo-Hye Nam
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  4. Yong-Taek Jung
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  5. Dong-Gyun Kim
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  6. Jung-Hoon Yoon
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Correspondence to Jung-Hoon Yoon.

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Kim, YO., Park, S., Nam, BH. et al. Ruegeria meonggei sp. nov., an alphaproteobacterium isolated from ascidian Halocynthia roretzi . Antonie van Leeuwenhoek 105, 551–558 (2014). https://doi.org/10.1007/s10482-013-0107-y

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