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Ruegeria arenilitoris sp. nov., isolated from the seashore sand around a seaweed farm

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Abstract

A Gram-negative, motile and rod-shaped bacterial strain, G-M8T, which was isolated from seashore sand around a seaweed farm at Geoje island in South Korea, was characterized taxonomically. It grew optimally at 30–37 °C, at pH 7.0–8.0 and in presence of 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain G-M8T joined the cluster comprising the type strains of Ruegeria atlantica and Ruegeria lacuscaerulensis, showing 97.5 % sequence similarity, by a bootstrap resampling value of 85.8 %. It exhibited 16S rRNA gene sequence similarity values of 95.4–96.7 % to the type strains of the other Ruegeria species. Strain G-M8T exhibited the highest gyrB sequence similarity value (88.5 %) to the type strain of R. lacuscaerulensis. Strain G-M8T contained Q-10 as the predominant ubiquinone and C18:1 ω7c as the predominant fatty acid. The polar lipid profile of strain G-M8T was similar to that of R. atlantica KCTC 12424T. The DNA G+C content of strain G-M8T was 64.6 mol% and its mean DNA–DNA relatedness values with R. atlantica KCTC 12424T and R. lacuscaerulensis KCTC 2953T were 18 ± 5.3 and 10 ± 3.6 %, respectively. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, demonstrated that strain G-M8T is distinguished from other Ruegeria species. On the basis of the data presented, strain G-M8T (=KCTC 23960T = CCUG 62412T) represents a novel species of the genus Ruegeria, for which the name Ruegeria arenilitoris sp. nov. is proposed.

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Acknowledgments

This work was supported by the Program for Collection, Management and Utilization of Biological Resources (grant M10867010003) and BK 21 program from the Ministry of Education, Science and Technology (MEST) of the Republic of Korea.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea

    Sooyeon Park & Jung-Hoon Yoon

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  1. Sooyeon Park
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  2. Jung-Hoon Yoon
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Correspondence to Jung-Hoon Yoon.

Additional information

The GenBank/EMBL/DDBJ accession numbers of the 16S rRNA gene sequence and gyrB sequence of Ruegeria arenilitoris G-M8T are JQ807219 and JQ807221, respectively.

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Park, S., Yoon, JH. Ruegeria arenilitoris sp. nov., isolated from the seashore sand around a seaweed farm. Antonie van Leeuwenhoek 102, 581–589 (2012). https://doi.org/10.1007/s10482-012-9753-8

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