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Identifying and characterizing Yarrowia keelungensis sp. nov., an oil-degrading yeast isolated from the sea surface microlayer

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Abstract

Ascomycetous yeast strain SM-22 was isolated from the sea-surface microlayer near the Keelung City off the northern coast of Taiwan. This strain showed a cell surface hydrophobicity higher than 90 %, moderate UV A/B resistance, and it degraded 68 % of the total petroleum hydrocarbon content of an artificial seawater medium containing 1 % (v v−1) diesel oil within 15 days at 25 °C. The closest phylogenetic relative of this strain is Candida oslonensis CBS 10146T, but it differs from strain SM-22 by a 3.7 % divergence (including 18 nucleotide substitutions and 2 gaps) in the D1/D2 domain sequence of the large subunit rRNA gene. This difference clearly suggests that the strain SM-22 represents a distinct species. Strain SM-22 does not produce ascospores on common sporulation media and it can therefore be considered an anamorph of the genus Yarrowia. Thus, the name Yarrowia keelungensis sp. nov. (type strain SM-22T = BCRC 23110T = JCM 14894T = CBS 11062T) is proposed as a novel species of genus Yarrowia.

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References

  • Abbasnezhad H, Gray M, Foght JM (2011) Influence of adhesion on aerobic biodegradation and bioremediation of liquid hydrocarbons. Appl Microbiol Biotechnol 92:653–675

    Article  PubMed  CAS  Google Scholar 

  • Amaral PFF, da Silva JM, Lehocky M, Barros-Timmons AMV, Coelho MAZ, Marrucho IM, Coutinho JAP (2006) Production and characterization of a bioemulsifier from Yarrowia lipolytica. Process Biochem 41:1894–1898

    Article  CAS  Google Scholar 

  • Bankar AV, Kumar AR, Zinjarde SS (2009) Environmental and industrial applications of Yarrowia lipolytica. Appl Microbiol Biotechnol 84:847–865

    Article  PubMed  CAS  Google Scholar 

  • Chang CF, Lee CF, Liu SM (2008) Cryptococcus keelungensis sp. nov., an anamorphic basidiomycetous yeast isolated from the sea-surface microlayer of the north-east coast of Taiwan. Int J Syst Evol Microbiol 58:2973–2976

    Article  PubMed  CAS  Google Scholar 

  • Chang CF, Lee CF, Liu SM (2010) Candida neustonensis sp. nov., a novel ascomycetous yeast isolated from the sea surface microlayer in Taiwan. Antonie Van Leeuwenhoek 97:35–40

    Article  PubMed  CAS  Google Scholar 

  • Chi ZM, Liu G, Zhao S, Li J, Peng Y (2010) Marine yeasts as biocontrol agents and producers of bio-products. Appl Microbiol Biotechnol 86:1227–1241

    Article  PubMed  CAS  Google Scholar 

  • Coelho MAZ, Amaral PFF, Belo I (2010) Yarrowia lipolytica: an industrial workhorse. In: Mendez-Vilas A (ed) Current research, technology and education topics in applied microbiology and microbial biotechnology, pp 930–944

  • Fell JW, Boekhout T, Fonseca A, Scorzetti G, Statzell-Tallman A (2000) Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50:1351–1371

    Article  PubMed  CAS  Google Scholar 

  • Garrett WD (1967) The organic chemical composition of the ocean surface. Deep Sea Res 14:221–227

    CAS  Google Scholar 

  • Groenewald M, Smith MT (2013) The teleomorph state of Candida deformans Langeron and Guerra and description of Yarrowia yakushimensis comb. nov. Antonie Van Leeuwenhoek 103:1023–1028

    Article  PubMed  CAS  Google Scholar 

  • Harvey GW, Burzell LA (1972) A simple microlayer methods for small samples. Limnol Oceanogr 17:156–157

    Article  Google Scholar 

  • Kim IS, Foght JM, Gray MR (2002) Selective transport and accumulation of alkanes by Rhodococcus erythropolis S + 14He. Biotechnol Bioeng 80:650–658

    Article  PubMed  CAS  Google Scholar 

  • Knutsen AK, Robert V, Poot GA, Epping W, Figge M, Holst-Jensen A, Skaar I, Smith MT (2007) Polyphasic reexamination of Yarrowia lipolytica strains and the description of three novel Candida species: Candida osloensis sp. nov., Candida alimentaria sp. nov. and Candida hollandica sp. nov. Int J Syst Evol Microbiol 57:2426–2435

    Article  PubMed  CAS  Google Scholar 

  • Kurtzman CP (2005) New species and a new combination in the Hyphopichia and Yarrowia yeast clades. Antonie Van Leeuwenhoek 88:121–130

    Article  PubMed  CAS  Google Scholar 

  • Kurtzman CP (2011) Yarrowia vander Walt, von Ary. In: Kurtzman CP, Fell JW, Boekhout T (eds) The yeasts, a taxonomic study, 5th edn. Elsevier, Amsterdam, pp 927–929

    Chapter  Google Scholar 

  • Kurtzman CP, Robnett CJ (1998) Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Van Leeuwenhoek 73:331–371

    Article  PubMed  CAS  Google Scholar 

  • Kurtzman CP, Fell JW, Boekhout T, Robert V (2011) Methods for isolation, phenotypic characterization and maintenance of yeasts. In: Kurtzman CP, Fell JW, Boekhout T (eds) The yeasts, a taxonomic study, 5th edn. Elsevier, Amsterdam, pp 137–144

    Chapter  Google Scholar 

  • Kutty SN, Philip R (2008) Marine yeasts—a review. Yeast 25:465–483

    Article  PubMed  CAS  Google Scholar 

  • Limtong S, Youngmanitchai W, Kawasaki H, Seki T (2008) Candida phangngensis sp. nov., an anamorphic yeast species in the Yarrowia clade, isolated from water in mangrove forest in Phang-Nga province Thailand. Int J Syst Evol Microbiol 58:515–519

    Article  PubMed  CAS  Google Scholar 

  • Maki JS (2002) Neuston microbiology: life at the air-water interface. In: Bitton G (ed) Encyclopedia of environmental microbiology. Wiley, New York, pp 2133–2144

    Google Scholar 

  • Margesin R, Schinner F (1997) Bioremediation of diesel-oil contaminated alpine soils at low temperatures. Appl Microbiol Biotechnol 47:46–462

    Article  Google Scholar 

  • Nakase T, Suzuki M (1988) Sporobolomyces yuccicola, a new species of ballistosporous yeast equipped with ubiquinone-9. Antonie Van Leeuwenhoek 54:47–55

    Article  PubMed  CAS  Google Scholar 

  • Obuekwe C, Al-Jadi ZK, Al-Saleh ES (2009) Hydrocarbon degradation in relation to cell surface hydrophobicity among bacterial hydrocarbon degraders from petroleum contaminated Kuwait desert environment. Int Biodeter Biodegrad 63:273–279

    Article  CAS  Google Scholar 

  • Péter G, Dlauchy D, Vasdinyei R, Tornai-Lehoczki J, Deak T (2004) Candida galli sp. nov., a new yeast from poultry. Antonie Van Leeuwenhoek 86:105–110

    Article  PubMed  Google Scholar 

  • Prillinger H, Lopandic K, Suzuki M, Kock JLF, Boekhout T (2011) Chemotaxonomy of yeasts. In: Kurtzman CP, Fell JW, Boekhout T (eds) The yeasts, a taxonomic study, 5th edn. Elsevier, Amsterdam, pp 77–100

    Google Scholar 

  • Romanovskaia VA, Tashirev AB, Shilin SO, Chernaia NA (2010) Resistance to UV radiation of microorganisms isolated from the rock biotopes of the Antarctic region. Mikrobiol Z 72:8–13

    Google Scholar 

  • Rosenberg M, Gutnick D, Rosenberg E (1980) Adherence of bacteria to hydrocarbons: a simple method for measuring cell surface hydrophobicity. FEMS Microbiol Lett 9:29–33

    Article  CAS  Google Scholar 

  • Tamaoka J, Komagata K (1984) Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128

    Article  CAS  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  PubMed  CAS  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  PubMed  CAS  Google Scholar 

  • Varnam AH, Malcolm GE (2000) Environmental Microbiology. American Society for Microbiology, Washington, D.C., USA

    Google Scholar 

  • Wang F, Yue L, Wang L, Madzak C, Li J, Wang X, Chi ZM (2009) Genetic modification of the marine-derived yeast Yarrowia lipolytica with high-protein content using a GPI-anchor-fusion expression system. Biotechnol Prog 25:1297–1303

    Article  PubMed  CAS  Google Scholar 

  • Zinjarde SS, Pant A (2002a) Hydrocarbon degraders from a tropical marine environment. Marine Poll Bull 44:118–121

    Article  CAS  Google Scholar 

  • Zinjarde SS, Pant A (2002b) Emulsifier from a tropical marine yeast, Yarrowia lipolytica NCIM 3589. J Basic Microbiol 42:67–73

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Graduate Institute of Marine Biology, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung, 20224, Taiwan

    Chin-Feng Chang, Chih-Chen Chen & Shiu-Mei Liu

  2. Department of Applied Science, National Hsinchu University of Education, 521 Nanda Road, Hsinchu, 30014, Taiwan

    Ching-Fu Lee

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  1. Chin-Feng Chang
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  2. Chih-Chen Chen
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  3. Ching-Fu Lee
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  4. Shiu-Mei Liu
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Correspondence to Shiu-Mei Liu.

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Chang, CF., Chen, CC., Lee, CF. et al. Identifying and characterizing Yarrowia keelungensis sp. nov., an oil-degrading yeast isolated from the sea surface microlayer. Antonie van Leeuwenhoek 104, 1117–1123 (2013). https://doi.org/10.1007/s10482-013-0033-z

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