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Diversity and dynamics of fungal endophytes in leaves, stems and roots of Stellera chamaejasme L. in northwestern China

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Abstract

This study was conducted to explore fungal endophyte communities inhabiting a toxic weed (Stellera chamaejasme L.) from meadows of northwestern China. The effects of plant tissue and growth stage on endophyte assemblages were characterized. Endophytes were recovered from 50 % of the samples, with a total of 714 isolates. 41 operational taxonomical units (OTUs) were identified, consisting of 40 OTUs belonging primarily to Ascomycota and 1 OTU belonging to Basidiomycota. Pleosporales and Hypocreales were the orders contributing the most species to the endophytic assemblages. The total colonization frequency and species richness of endophytic fungi were higher in roots than in leaves and stems. In addition, for the plant tissues, the structure of fungal communities differed significantly by growth stages of leaf emergence and dormancy; for the plant growth stages, the structure of fungal communities differed significantly by plant tissues. This study demonstrates that S. chamaejasme serves as a reservoir for a wide variety of fungal endophytes that can be isolated from various plant tissues.

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References

  • Angelini P, Rubini A, Gigante D, Reale L, Pagiotti R, Venanzoni R (2012) The endophytic fungi communities associated with the leaves and roots of the common reed (Phragmites australis) in lake Trasimeno (Perugia, Italy) in declining and healthy stands. Fungal Ecol 5:683–693

    Article  Google Scholar 

  • Arnold AE (2007) Understanding the diversity of foliar endophytic fungi: progress, challenges, and frontiers. Fungal Biol Rev 21:51–66

    Article  Google Scholar 

  • Arnold AE, Mejia LC, Kyllo D, Rojas EI, Maynard Z, Robbins N, Herre EA (2003) Fungal endophytes limit pathogen damage in a tropical tree. Pros Natl Acad Sci USA 100:15649–15654

    Article  CAS  Google Scholar 

  • Ashelford KE, Chuzhanova NA, Fry JC, Jones AJ, Weightman AJ (2005) At least 1 in 20 16S rRNA sequence records currently held in public repositories is estimated to contain substantial anomalies. Appl Environ Microb 71:7724–7736

    Article  CAS  Google Scholar 

  • Beraldi-Campesi H, Arenas-Abad C, Garcia-Pichel F, Arellano-Aguilar O, Auqué L, Vázquez-Urbez M, Sancho C, Osácar C, Ruiz-Velasco S (2012) Benthic bacterial diversity from freshwater tufas of the Iberian Range (Spain). FEMS Microb Ecol 80:363–379

    Article  CAS  Google Scholar 

  • Cole JR, Chai B, Farris RJ, Wang Q, Kulam-Syed-Mohideen AS, McGarrell DM, Bandela AM, Cardenas E, Garrity GM, Tiedje JM (2007) The ribosomal database project (RDP-II): introducing myRDP space and quality controlled public data. Nucleic Acids Res 35 (database issue):D169–D172

    Article  Google Scholar 

  • Ding G, Liu S, Guo L, Zhou Y, Che Y (2008) Antifungal metabolites from the plant endophytic fungus Pestalotiopsis foedan. J Nat Prod 71:615–618

    Article  PubMed  CAS  Google Scholar 

  • Garbeva P, Van Veen JA, Van Elsas JD (2004) Microbial diversity in soil: selection of microbial population by plant and soil type and implications for disease suppressiveness. Annu Rev Phytopathol 42:243–270

    Article  PubMed  CAS  Google Scholar 

  • Ghimire SR, Charlton ND, Bell JD, Krishnamurthy YL, Craven KD (2011) Biodiversity of fungal endophyte communities inhabiting switchgrass (Panicum virgatumL.) growing in the native tallgrass prairie of northern Oklahoma. Fungal Divers 47:19–27

    Article  Google Scholar 

  • Guo LD, Huang GR, Wang Y (2008) Seasonal and tissue age influences on endophytic fungi of Pinus tabulaeformis (Pinaceae) in the Dongling Mountains, Beijing. J Integr Plant Biol 50:997–1003

    Article  PubMed  Google Scholar 

  • Guo X, Ding WJ, Yang JY, Xu R, Yan ZQ, Qin B (2011) Studies on the nematicidal effective fraction of Stellera chamaejasme root extracts to Ditylenchus destructor. Plant Protection 31:128–131 (in Chinese with English abstract)

    Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41:95–98

    CAS  Google Scholar 

  • Hamayun M, Khan SA, Iqbal I, Ahmad B, Lee I-J (2010) Isolation of a gibberellin-producing fungus (Penicillium sp. MH7) and growth promotion of crown caisy (Chrysanthemum coronarium). J Microbiol Biotechnol 20:202–207

    PubMed  CAS  Google Scholar 

  • Herrera J, Khidir HH, Eudy DM, Porras-Alfaro A, Natvig DO, Sinsabaugh RL (2010) Shifting fungal endophyte communities colonize Bouteloua gracilis: effect of host tissue and geographical distribution. Mycologia 102:1012–1026

    Article  PubMed  Google Scholar 

  • Huang WY, Cai YZ, Surveswaran S, Hyde KD, Corke H, Sun M (2009) Molecular phylogenetic identification of endophytic fungi isolated from three Artemisia species. Fungal Divers 36:69–88

    CAS  Google Scholar 

  • Hyde KD, Soytong K (2008) The fungal endophyte dilemma. Fungal Divers 33:163–173

    Google Scholar 

  • Hyde KD, Bussaban B, Paulus B, Crous PW, Lee S, Mckenzie EHC, Photita W, Lumyong S (2007) Diversity of saprobic microfungi. Biodivers Conserv 16:17–35

    Article  Google Scholar 

  • Joshee S, Paulus BC, Park D, Johnston PR (2009) Diversity and distribution of fungal foliar endophytes in New Zealand Podocarpaceae. Mycol Res 113:1003–1015

    Article  PubMed  Google Scholar 

  • Kharwar RN, Verma VC, Kumar A, Gond SK, Strobel G (2009) Javanicin, an antibacterial naphthaquinone from an endophytic fungus of Neem, Chloridium sp. Curr Microbiol 58:233–238

    Google Scholar 

  • Kumar DSS, Hyde KD (2004) Biodiversity and tissue-recurrence of endophytic fungi in Tripterygium wilfordii. Fungal Divers 17:69–90

    CAS  Google Scholar 

  • Kumara PM, Zuehlke S, Priti V, Ramesha BT, Shweta S, Ravikanth G, Vasudeva R, Santhoshkumar TR, Spiteller M, Shaanker RU (2012) Fusarium proliferatum, an endophytic fungus from Dysoxylum binectariferum Hook.f, produces rohitukine, a chromane alkaloid possessing anti-cancer activity. Antonie van Leeuwenhoek 101:323–329

    Article  CAS  Google Scholar 

  • Kutorga E, Adamonytė G, Iršėnaitė R, Juzėnas S, Kasparavičius J, Markovskaja S, Motiejūnalitė J, Treigienė A (2012) Wildfire and post-fire management effects on early fungal succession in pinus mugo plantations, located in Curonian Spit (Lithuania). Geoderma 191:70–79

    Article  Google Scholar 

  • Lockwood JL (1981) Exploitation competion. In: Wicklow DT, Carrol GC (eds) The fungal community: its organization and role in ecosystem. Marcel Dekker, New York, pp 319–349

    Google Scholar 

  • Martin LL, Ross Friedman CM, Phillips LA (2012) Fungal endophytes of the obligate parasitic dwarf mistletoe Arceuthobium americanum (Santalaceae) act antagonistically in vitro against the native fungal pathogen Cladosporium (Davidiellaceae) of their host. Am J Bot 99:2027–2034

    Article  PubMed  Google Scholar 

  • Menjivar RD, Cabrera JA, Kranz J, Sikora RA (2012) Induction of metabolite organic compounds by mutualistic endophytic fungi to reduce the greenhouse whitefly Trialeurodes vaporariorum (Westwood) infection on tomato. Plant Soil 352:233–241

    Article  CAS  Google Scholar 

  • Mishra A, Gond SK, Kumar A, Sharma VK, Verma SK, Kharwar RN, Sieber TN (2012) Season and tissue type affect fungal endophyte communities of the Indian medicinal plant Tinospora cordifolia more strongly than geographic location. Microb Ecol 64:388–398

    Article  PubMed  Google Scholar 

  • Morakotkarn D, Kawasaki H, Seki T (2006) Molecular diversity of bamboo-associated fungi isolated from Japan. FEMS Microbiol Lett 266:10–19

    Article  PubMed  Google Scholar 

  • O’Brien HE, Parrent JL, Jackson JA, Moncalvo J-M, Vi lgalys R (2005) Fungal community analysis by large-scale sequencing of environmental samples. Appl Environ Microbiol 71:5544–5550

    Article  PubMed  Google Scholar 

  • Oechsler RA, Feilmeier MR, Ledee DR, Miller D, Diaz MR, Elizabeth Fini M, Fell JW, Alfonso EC (2009) Utility of molecular sequence analysis of the ITS rRNA region for identification of Fusarium spp. from ocular sources. Invest Ophth Vis Sci 50:2230–2236

    Article  Google Scholar 

  • Porras-Alfaro A, Herrera J, Natvig DO, Sinsabaugh RL (2007) Effect of long term nitrogen fertilization on mycorrhizal fungi associated with a dominant grass in a semiarid grassland. Plant Soil 296:65–75

    Article  CAS  Google Scholar 

  • Quilliam RS, Jones DL (2010) Fungal root endophytes of the carnivorous plant Drosera rotundifolia. Mycorrhiza 20:341–348

    Article  PubMed  Google Scholar 

  • Rakotoniriana EF, Munaut F, Decock C, Randriamampionona D, Andriambololoniaina M, Rakotomalala T, Rakotonirina EJ, Rabemanantsoa C, Cheuk K, Ratsimamanga SU, Mahillon J, EI-Jaziri M, Quetin-Leclercq J, Corbisier AM (2008) Endophytic fungi from leaves of Centella asiatica: occurrence and potential interactions within leaves. Antonie van Leeuwenhoek 93:27–36

    Article  PubMed  CAS  Google Scholar 

  • Raviraja NS, Maria GL, Sridhar KR (2006) Antimicrobial evaluation of endophytic fungi inhabiting medicinal plants of the Western Ghats of India. Eng Life Sci 6:515–520

    Article  CAS  Google Scholar 

  • Rivera-Orduña FN, Suarez-Sanchez RA, Flores-Bustamante ZR, Gracida-Rodriguez JN, Flores-Cotera LB (2011) Diversity of endophytic fungi of Taxus globosa (Mexican yew). Fungal Divers 47:65–74

    Article  Google Scholar 

  • Rodrigues KF (1994) The foliar fungal endophytes of the Amazonian Palm Euterpe oleracea. Mycologia 86:376–385

    Article  Google Scholar 

  • Rosa LH, Vaz ABM, Caligiorne RB, Campolina S, Rosa CA (2009) Endophytic fungi associated with the Antarctic grass Deschampsia antarctica Desv. (Poaceae). Polar Biol 32:161–167

    Article  Google Scholar 

  • Sánchez Márquez S, Bills GF, Domínguez Acuña L, Zabalgogeazcoa I (2010) Endophytic mycobiota of leaves and roots of the grass Holcus lanatus. Fungal Divers 41:115–123

    Article  Google Scholar 

  • Saunders M, Kohn LM (2009) Evidence for alteration of fungal endophyte community assembly by host defense compounds. New Phytol 182:229–238

    Article  PubMed  CAS  Google Scholar 

  • Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ, Sahl JW, Stres B, Thallinger GG, Van Horn DJ, Weber CF (2009) Introducing mothur: opensource, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 75:7537–7541

    Article  PubMed  CAS  Google Scholar 

  • Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, André levesque C, Chen W, Fungal Barcoding Consortium (2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Pros Natl Acad Sci USA 109:6241–6246

    Article  CAS  Google Scholar 

  • Schulthess FM, Faeth SH (1998) Distribution, abundances and association of the endophytic fungal community of Arizona fescue (Festuca arizonica). Mycologia 90:569–578

    Article  Google Scholar 

  • Schulz B, Guske S, Dammann U, Boyle C (1998) Endophyte-host interactions II. Defining symbiosis of the endophyte-host interaction. Symbiosis 25:213–227

    Google Scholar 

  • Schulz B, Boyle C, Draeger S, Römmert AK, Krohn K (2002) Endophytic fungi: a source of novel biologically active secondary metabolites. Mycol Res 106:996–1004

    Article  CAS  Google Scholar 

  • Shankar NB, Shashikala J, Krishnamurthy YL (2007) Study on diversity of endophytic communities from rice (Oryza sativa L.) and their antagonistic activities in vitro. Microbiol Res 164:290–296

    Google Scholar 

  • Shannon CE, Weaver W (1963) The mathematical theory of communication. University of Illinois Press, Urbana

    Google Scholar 

  • Sieber TN, Hugentobler C (1987) Endophytische Pilze in Blättern und Ästen gesunder und geschädigter Buchen (Fagus sylvatica L.). Eur J Forest Pathol 17:411–425

    Article  Google Scholar 

  • Sieber T (2007) Endophytic fungi in forest trees: are they mutualists? Fungal Biol Rev 21:75–89

    Article  Google Scholar 

  • Sim HJ, Khoo CH, Lee LH, Cheah YK (2010) Molecular diversity of fungal endophytes isolated from Garcinia mangostana and Garcinia parvifolia. J Microbiol Biotechn 20:651–658

    Article  CAS  Google Scholar 

  • Simpson GG (1964) Species density of North American recent mammals. Syst Zool 13:57–73

    Article  Google Scholar 

  • Stierle A, Strobel G, Stierle D (1993) Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew. Science 260:214–216

    Article  PubMed  CAS  Google Scholar 

  • Sun G, Luo P, Wu N, Qiu PF, Gao YH, Chen H, Shi FS (2009) Stellera chamaejasme L. increases soil N availability, turnover rates and microbial biomass in an alpine meadow ecosystem on the eastern Tibetan Plateau of China. Soil Biol Biochem 41:86–91

    Article  Google Scholar 

  • Sun X, Guo LD, Hyde KD (2011) Community composition of endophytic fungi in Acer truncatum and their role in decomposition. Fungal Divers 47:85–95

    Article  Google Scholar 

  • Sun X, Ding Q, Hyde KD, Guo LD (2012) Community structure and preference of endophytic fungi of three woody plants in a mixed forest. Fungal Ecol 5:624–632

    Article  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol biol evol 28:2731–2739

    Article  PubMed  CAS  Google Scholar 

  • Tao G, Liu ZY, Hyde KD, Lui XZ, Yu ZN (2008) Whole rDNA analysis reveals novel and endophytic fungi in Bletilla ochracea (Orchidaceae). Fungal Divers 33:101–122

    Google Scholar 

  • Vega FE, Simpkins A, Aime MC, Posada F, Pehner SA, Infante F, Castillo A, Arnold AE (2010) Fungal endophyte diversity in coffee plants from Colombia, Hawai’i, Mexico and Puerto Rico. Fungal Ecol 3:122–138

    Article  Google Scholar 

  • Wang Y, Guo LD, Hyde KD (2005) Taxonomic placement of sterile morphotypes of endophytic fungi from Pinus tabulaeformis (Pinaceae) in northeast China based on rDNA sequences. Fungal Divers 20:235–260

    CAS  Google Scholar 

  • Wang Y, Wnag G, Wang L, Xu X, Xia J, Huang X, Wu Y, Zhang C (2010) Isolation and identification of an endophytic fungus of Polygonatum cyrtonema and antifungal metabolites. Acta Microbiol Sinica 50:1036–1043 (in Chinese with English abstract)

    CAS  Google Scholar 

  • Wearn JA, Sutton BC, Morley NJ, Gange AC (2012) Species and organ specificity of fungal endophytes in herbaceous grassland plants. J Ecol 100:1085–1092

    Article  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–322

    Google Scholar 

  • Widler B, Müller E (1984) Untersuchungen über endophytische Pilze von Arctostaphylos uva-ursi (L.) Sprengel (Ericaceae). Bot Helv 94:307–337

    Google Scholar 

  • Yang S, Zhang W, Shi JS, Shi GY, Qin SG, Chang ZL, Chen Y, Wang ZH, Huang JP (2010) Preliminary analyses of the characteristics of black carbon in the semi-arid area. Clim Environ Res 15:756–764 (in Chinese with English abstract)

    Google Scholar 

  • Yang JY, Yan ZQ, Xu R, Liu Q, Jin H, Cui HY, Qin B (2011) Isolation of plant growth inhibitory components from the root of Stellera chamaejasme and function mechanism. Acta Botanica Boreali-Occidentalia Sinica 31:0291–0297 (in Chinese with English abstract)

    CAS  Google Scholar 

  • Yuan Z, Zhang C, Lin F, Kubicek CP (2010) Identity, diversity, and molecular phylogeny of the endophytic mycobiota in the roots of rare wild rice (Oryza granulate) from a nature reserve in Yunnan, China. Appl Environ Microbiol 76:1642–1652

    Article  PubMed  CAS  Google Scholar 

  • Zamora P, Martínez-Ruiz C, Diez JJ (2008) Fungi in needles and twigs of pine plantations from northern Spain. Fungal Divers 30:171–184

    Google Scholar 

  • Zhang ZQ, Zhang YH, Sun H (2011) The reproductive biology of Stellera chamaejasme (Thymelaeaceae): a self-incompatible weed with specialized flowers. Flora 206:567–574

    Article  Google Scholar 

  • Zhou X, Wang Z, Jiang K, Wei Y, Lin J, Sun X, Tang K (2007) Screening of taxol producing endophytic fungi from Taxus chinensis var. mairei. Appl Biochem Microbiol 43:439–443

    Article  CAS  Google Scholar 

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (No. 310703863 and No. 21102154), Basic Research Program of Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (080423SYR1), and the associate scholar program for talents cultivation plan of “Western Light” of Chinese Academy of Sciences.

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  1. Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, China

    Hui Jin, Zhiqiang Yan, Quan Liu, Xiaoyan Yang & Bo Qin

  2. School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou, 730050, China

    Jixiang Chen

  3. State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, 730000, China

    Bo Qin

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  1. Hui Jin
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Correspondence to Bo Qin.

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Jin, H., Yan, Z., Liu, Q. et al. Diversity and dynamics of fungal endophytes in leaves, stems and roots of Stellera chamaejasme L. in northwestern China. Antonie van Leeuwenhoek 104, 949–963 (2013). https://doi.org/10.1007/s10482-013-0014-2

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