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Desulfosoma profundi sp. nov., a thermophilic sulfate-reducing bacterium isolated from a deep terrestrial geothermal spring in France

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Abstract

A novel strictly anaerobic bacterium designated SPDX02-08T was isolated from a deep terrestrial geothermal spring located in southwest France. Cells (1–2 × 2–6 μm) were non-motile, non sporulating and stained Gram negative. Strain SPDX02-08T grew at a temperature between 40 and 60°C (optimum 55°C), pH between 6.3 and 7.3 (optimum 7.2) and a NaCl concentration between 0 and 5 g/l (optimum 2 g/l). Sulfate, thiosulfate and sulfite were used as terminal electron acceptors, but not elemental sulfur, nitrate, nitrite, Fe (III) or fumarate. In the presence of sulfate, strain SPDX02-08T completely oxidized pyruvate, propionate, butyrate, isobutyrate, valerate, isovalerate and hexadecanoate. Stoichiometric measurements revealed a complete oxidation of part of lactate (0.125 mol of acetate produced per mole lactate oxidized). Strain SPDX02-08T required yeast extract to oxidize formate and H2 but did not grow autotrophically on H2. Among the substrates tested, only pyruvate was fermented. The G+C content of the genomic DNA was 57.6 mol%. Major cellular fatty acids of strain SPDX02-08T were iso-C15:0, C15:0, and C16:0. Phylogenetic analysis of the 16S small-subunit (SSU) ribosomal RNA gene sequence indicated that strain SPDX02-08T belongs to the genus Desulfosoma, family Syntrophobacteraceae, having Desulfosoma caldarium as its closest phylogenetic relative (97.6% similarity). The mean DNA/DNA reassociation value between strain SPDX02-08T and Desulfosoma caldarium was 16.9 ± 2.7%. Based on the polyphasic differences, strain SPDX02-08T is proposed to be assigned as a new species of the genus Desulfosoma, Desulfosoma profundi sp. nov. (DSM 22937T = JCM 16410T). GenBank accession number for the 16S rRNA gene sequence of strain SPDX02-08T is HM056226.

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Acknowledgments

This work was supported by grants from AFRETH and Institut du Thermalisme – Université Victor Segalen Bordeaux 2. Many thanks to Dr Anne C. Frazer (Plant and Microbial Biology Department, University of California of Berkeley) for improving the manuscript, to the Mayor of Saint-Paul-Lès-Dax for having given us access to the hot spring and to Dr J. P. Euzéby for checking the etymology of the species name.

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Authors and Affiliations

  1. Laboratoire de Microbiologie et de Biotechnologie des Environnements Chauds UMR 180, IRD, Universités de Provence et de la Méditerranée, ESIL Case 925, 163 Avenue de Luminy, 13288, Marseille Cedex 9, France

    Patrick Grégoire, Marie-Laure Fardeau & Bernard Ollivier

  2. Institut du Thermalisme, Université Victor Segalen Bordeaux 2, 8 rue Sainte Ursule, 40100, Dax, France

    Patrick Grégoire, Joël Lagière & Jean Cambar

  3. Laboratoire de Microbiologie, de Géochimie et d’Ecologie Marine, Centre d’Océanologie de Marseille, Université de la Méditerranée, Aix Marseille II, CNRS-UMR 6117 Campus de Luminy, Case 901, 13009, Marseille Cedex 9, France

    Sophie Guasco, Valérie Michotey & Patricia Bonin

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  1. Patrick Grégoire
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  2. Marie-Laure Fardeau
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  3. Sophie Guasco
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  8. Bernard Ollivier
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Correspondence to Bernard Ollivier.

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Grégoire, P., Fardeau, ML., Guasco, S. et al. Desulfosoma profundi sp. nov., a thermophilic sulfate-reducing bacterium isolated from a deep terrestrial geothermal spring in France. Antonie van Leeuwenhoek 101, 595–602 (2012). https://doi.org/10.1007/s10482-011-9675-x

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  • DOI: https://doi.org/10.1007/s10482-011-9675-x

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