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Desulfosporosinus acididurans sp. nov.: an acidophilic sulfate-reducing bacterium isolated from acidic sediments

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Abstract

Three strains of sulfate-reducing bacteria (M1T, D, and E) were isolated from acidic sediments (White river and Tinto river) and characterized phylogenetically and physiologically. All three strains were obligately anaerobic, mesophilic, spore-forming straight rods, stained Gram-negative and displayed variable motility during active growth. The pH range for growth was 3.8–7.0, with an optimum at pH 5.5. The temperature range for growth was 15–40 °C, with an optimum at 30 °C. Strains M1T, D, and E used a wide range of electron donors and acceptors, with certain variability within the different strains. The nominated type strain (M1T) used ferric iron, nitrate, sulfate, elemental sulfur, and thiosulfate (but not arsenate, sulfite, or fumarate) as electron acceptors, and organic acids (formate, lactate, butyrate, fumarate, malate, and pyruvate), alcohols (glycerol, methanol, and ethanol), yeast extract, and sugars (xylose, glucose, and fructose) as electron donors. It also fermented some substrates such as pyruvate and formate. Strain M1T tolerated up to 50 mM ferrous iron and 10 mM aluminum, but was inhibited by 1 mM copper. On the basis of phenotypic, phylogenetic, and genetic characteristics, strains M1T, D, and E represent a novel species within the genus Desulfosporosinus, for which the name Desulfosporosinus acididurans sp. nov. is proposed. The type strain is M1T (=DSM 27692T = JCM 19471T). Strain M1T was the first acidophilic SRB isolated, and it is the third described species of acidophilic SRB besides Desulfosporosinus acidiphilus and Thermodesulfobium narugense.

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Acknowledgments

This work was financed by an ERC Grant (project 323009) attributed to A.J.M. Stams and the Gravitation Grant (SIAM 024.002.002) of the Netherlands Ministry of Education, Culture and Science and the Netherlands Science Foundation. Thanks to Dr. Martin Mühling, Dr. Patrick Petzsch and Prof. Michael Schlömann for sharing the data of the genome sequencing funded by the European Social Fund (ESF) and the Federal State of Saxony (Germany) and to Prof. Ricardo Amils and Prof. Jose Luis Sanz to promote the isolation of strains D and E.

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Authors and Affiliations

  1. Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB, Wageningen, The Netherlands

    Irene Sánchez-Andrea & Alfons J. M. Stams

  2. Federal Institute for Geosciences and Natural Resources (BGR), Stilleweg 2, 30655, Hannover, Germany

    Sabrina Hedrich

  3. Universidad Arturo Prat, Iquique, Chile & Instituto Tecnológico Vale, Belém, Brazil

    Ivan Ňancucheo

  4. College of Natural Sciences, Bangor University, Bangor, LL57 2UW, UK

    D. Barrie Johnson

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  1. Irene Sánchez-Andrea
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  2. Alfons J. M. Stams
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  3. Sabrina Hedrich
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  4. Ivan Ňancucheo
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  5. D. Barrie Johnson
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Correspondence to Irene Sánchez-Andrea.

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Communicated by A. Oren.

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Sánchez-Andrea, I., Stams, A.J.M., Hedrich, S. et al. Desulfosporosinus acididurans sp. nov.: an acidophilic sulfate-reducing bacterium isolated from acidic sediments. Extremophiles 19, 39–47 (2015). https://doi.org/10.1007/s00792-014-0701-6

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