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Halovibrio salipaludis sp. nov., Isolated from Saline-Alkaline Soil

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Abstract

A Gram-staining-negative, halophilic, aerobic, oval-shaped or vibrio-shaped, motile by a polar flagellum strain, designated YL5-2T, was isolated from natural saline-alkaline wetland soil of Binhai new district, Tianjin, China. Strain YL5-2T grew optimally at 35 °C, pH 7.5–8.0, and in the presence of 10–25% (w/v) NaCl on MA medium. Phylogenetic analyses based on 16S rRNA gene sequences showed that the isolate belonged to the genus Halovibrio and exhibited high sequence similarity of 97.7% to Halovibrio variabilis DSM 3050T. The sole respiratory ubiquinone of strain YL5-2T is Q-9, and the dominant fatty acids were C18:1ω9c, C16:0, C19:0 cycloω8c, and Summed Feature 8. The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), and lipid (L). The DNA G+C content of the strain was 62.1 mol%. The average nucleotide identity (ANI) based on whole genome sequences of strain YL5-2T and Halovibrio variabilis DSM 3050T was 93.85%, and the dDDH value between these two strains was determined to be 52.0%. Phenotypic, chemotaxonomic, phylogenetic, and genomic analyses suggested that strain YL5-2T represent a novel species of the genus Halovibrio, for which the name Halovibrio salipaludis sp. nov. is proposed. The type strain is YL5-2T (=KCTC 52852T=ACCC 19971T).

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Acknowledgements

The authors would like to thank Prof. Aharon Oren for very valuable help in naming the organism.

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Authors and Affiliations

  1. College of Life Science, Yangtze University, Hubei, 434025, People’s Republic of China

    Liu Tang, Hanxu Liu & Lian Ma

  2. China Bluestar Lehigh Engineering Institute Co., Ltd., Jiangsu, 222004, People’s Republic of China

    Wenni Sun

  3. Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affair, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China

    Liu Tang, Hanxu Liu, Juanjuan Zhao, Shaohua Miao, Qi Gong & Guishan Zhang

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Contributions

LT contributed to performing the experiments and writing the initial draft. WS provided samples of experiment and participated in the isolation and cultivation of strains. GZ and LM contributed to the guidance of experimental operations. HL and SM contributed to the morphological analyses. JZ and QG performed genome analysis. GZ and LM contributed to reagents, instrumentation, and the financial support for this work. All the authors approved the manuscript.

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Correspondence to Lian Ma or Guishan Zhang.

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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene of strain YL5-2T is MF782425. The whole genome has been deposited at DDBJ/EMBL/GenBank under the accession NSKD00000000 for strain YL5-2T. Thin-layer chromatograms of the polar lipids extracted from strain YL5-2T and closely related species, additional phylogenetic trees, and the table containing the average nucleotide identity (ANI), and dDDH values to closely related genomes are available as Supplementary Materials.

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Tang, L., Liu, H., Sun, W. et al. Halovibrio salipaludis sp. nov., Isolated from Saline-Alkaline Soil. Curr Microbiol 78, 429–434 (2021). https://doi.org/10.1007/s00284-020-02282-y

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  • DOI: https://doi.org/10.1007/s00284-020-02282-y

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