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Alteromonas halophila sp. nov., a new moderately halophilic bacterium isolated from a sea anemone

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Abstract

A pale yellow-colored, moderately halophilic, Gram-negative, catalase- and oxidase-positive, non-sporulating, rod-shaped, motile, aerobic bacterium, designated strain JSM 073008T, was isolated from a sea anemone (Anthopleura xanthogrammica) collected from Naozhou Island, Leizhou Bay, South China Sea. The organism was able to grow with 1–20% (w/v) total salts (optimum, 5–10%), at pH 6.0–10.0 (optimum, pH 7.5) and 10–40°C (optimum, 25–30°C). The major cellular fatty acids were C16:0, C16:1 ω7c/iso-C15:0 2-OH and C18:1 ω7c. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid. The predominant respiratory quinone was Q-8 and the genomic DNA G + C content was 47.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 073008T should be assigned to the genus Alteromonas, being most closely related to Alteromonas hispanica F-32T (sequence similarity 96.9%), followed by Alteromonas genovensis LMG 24078T (96.6%) and Alteromonas litorea TF-22T (96.4%). The sequence similarities between the novel isolate and the type strains of other recognized Alteromonas species ranged from 95.9% (with Alteromonas stellipolaris ANT 69aT) to 94.5% (with Alteromonas simiduii BCRC 17572T). The combination of phylogenetic analysis, phenotypic characteristics and chemotaxonomic data supported the view that strain JSM 073008T represents a new species of the genus Alteromonas, for which the name Alteromonas halophila sp. nov. is proposed. The type strain is JSM 073008T (=CCTCC AA 207035T = KCTC 22164T).

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (NSFC) (30860013, 30860002, 30660004), Jishou University (jsdxkyzz200811), the Ministry of Science and Technology, PR China (2006BAE01A01-9; 863 Program, no. 2007AA021306), Key Projects of International Cooperation (2007DFB31620) and Yunnan University (2008YB005). We are grateful to Mr Ke Huang for his excellent technical assistance.

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Authors and Affiliations

  1. College of Biology and Environmental Science, Department of Financial Management, Jishou University, 416000, Jishou, People’s Republic of China

    Yi-Guang Chen, Huai-Dong Xiao, Entomack Borrathybay & Yan-Qi Liu

  2. The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, 650091, Kunming, People’s Republic of China

    Yi-Guang Chen, Shu-Kun Tang, Xiao-Long Cui & Wen-Jun Li

  3. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, 100050, Beijing, People’s Republic of China

    Yu-Qin Zhang

  4. Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 510301, Guangzhou, People’s Republic of China

    Wen-Jun Li

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  1. Yi-Guang Chen
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Correspondence to Wen-Jun Li or Yan-Qi Liu.

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The authors Yi-Guang Chen and Huai-Dong Xiao have contributed equally to this work.

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Chen, YG., Xiao, HD., Tang, SK. et al. Alteromonas halophila sp. nov., a new moderately halophilic bacterium isolated from a sea anemone. Antonie van Leeuwenhoek 96, 259–266 (2009). https://doi.org/10.1007/s10482-009-9341-8

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