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Arthrobacter halodurans sp. nov., a new halotolerant bacterium isolated from sea water

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Abstract

A novel Gram-positive, halotolerant, non-sporulating, non-motile, catalase-positive, oxidase-negative and aerobic bacterium, designated strain JSM 078085T, was isolated from sea water collected from the South China Sea. Strain JSM 078085T exhibited a rod-coccus growth cycle and produced a yellow pigment. The strain was able to grow in the presence of 0–12% (w/v) NaCl and at pH 6.0–9.5 and 4–35°C; optimum growth was observed at pH 7.0 and 25–30°C in the absence of NaCl. The peptidoglycan type was A4α (l-Lys–l-Ala–l-Glu). Cell-wall sugars contained galactose and glucose. Strain JSM 078085T contained menaquinone MK-9(H2) as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as the major polar lipids. The major cellular fatty acids were anteiso-C15:0, iso-C15:0 and anteiso-C17:0 and the DNA G + C content was 63.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 078085T should be assigned to the genus Arthrobacter, being most closely related to the type strain of Arthrobacter rhombi (sequence similarity 97.1%), and the two strains formed a distinct lineage in the phylogenetic tree. The level of DNA–DNA relatedness between strain JSM 078085T and the type strain of Arthrobacter rhombi was 10.6%. The combination of phylogenetic analysis, DNA–DNA relatedness, phenotypic characteristics and chemotaxonomic data supported the view that strain JSM 078085T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter halodurans sp. nov. is proposed. The type strain is JSM 078085T (=DSM 21081T=KCTC 19430T).

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (NSFC) (30860013, 30860002, 30660004), Jishou University (jsdxkyzz200811), the Ministry of Science and Technology, PR China (2006BAE01A01-9, 863 Program, no. 2007AA021306), Key Projects of International Cooperation (2007DFB31620), the Yunnan Provincial Sciences and Technology Department (2005PY01-1, 2006C0006 M) and Yunnan University (2008YB005). We are grateful to Mr. Huai-Dong Xiao and Mr. Ke Huang for their excellent technical assistance.

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Authors and Affiliations

  1. Key Laboratory for Conservation and Utilization of Plant Resources of Hunan Province, College of Biology and Environmental Sciences, Jishou University, 416000, Jishou, People’s Republic of China

    Yi-Guang Chen, Zhao-Yang Li & Lang-Bo Yi

  2. The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, 650091, Kunming, People’s Republic of China

    Yi-Guang Chen, Shu-Kun Tang, Yu-Qin Zhang, Yong-Xia Wang, Wen-Jun Li & Xiao-Long Cui

  3. Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, 650091, Kunming, People’s Republic of China

    Yi-Guang Chen, Shu-Kun Tang, Yu-Qin Zhang, Yong-Xia Wang, Wen-Jun Li & Xiao-Long Cui

  4. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, 100050, Beijing, People’s Republic of China

    Yu-Qin Zhang

  5. Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 510301, Guangzhou, People’s Republic of China

    Wen-Jun Li

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  1. Yi-Guang Chen
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Correspondence to Yi-Guang Chen.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JSM 078085T is EU583729.

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Chen, YG., Tang, SK., Zhang, YQ. et al. Arthrobacter halodurans sp. nov., a new halotolerant bacterium isolated from sea water. Antonie van Leeuwenhoek 96, 63–70 (2009). https://doi.org/10.1007/s10482-009-9336-5

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