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Studies on bacteriocin (thermophilin T) production by Streptococcus thermophilus ACA-DC 0040 in batch and fed-batch fermentation modes

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Abstract

Growth conditions that support bacteriocin (thermophilin T) production by Streptococcus thermophilus ACA-DC 0040 were identified. Synthesis of thermophilin T occurred during primary metabolic growth, while its specific rate of synthesis seemed to be optimal at T = 30°C. Thermophilin T activity rapidly decreased in the stationary phase, especially at high growth temperature (i.e. T = 42°C). In media with high content of complex nitrogen sources, high amounts of bacteriocin were detected in the growth environment, while about an 8-fold increase of thermophilin T titer and a 2-fold increase of specific synthesis rate was achieved when a fed-batch fermentation mode was applied.

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Abbreviations

α :

Specific bacteriocin production (AU g−1)

AU:

Arbitrary units

B :

Bacteriocin activity (titer) per volume of growth medium (MAU l−1)

B max :

Maximum bacteriocin activity (MAU l−1)

CNS:

Complex nitrogen source concentration (g l−1)

LAB:

Lactic acid bacteria

q B :

Specific bacteriocin production rate (h−1)

r :

Specific growth rate (h−1)

r max :

Maximum specific growth rate (h−1)

r D :

Specific bacteriocin inactivation rate (h−1)

x :

Biomass (cell dry mass) concentration (g l−1)

x max :

Maximum biomass concentration (g l−1)

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Authors and Affiliations

  1. Laboratory of Dairy Research, Department of Food Science and Technology, Agricultural University of Athens, Iera Odos 75, Athens, 11855, Greece

    Anastasios Aktypis & George Kalantzopoulos

  2. Department of Food Engineering, Agricultural University of Wroclaw, Nowodworska 99/13, Wroclaw, 54438, Poland

    Matheus Tychowski

  3. Division of Genetics, Cell and Development Biology, Department of Biology, University of Patras, Patras, 26504, Greece

    George Aggelis

Authors
  1. Anastasios Aktypis
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  2. Matheus Tychowski
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  3. George Kalantzopoulos
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  4. George Aggelis
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Correspondence to Anastasios Aktypis.

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Aktypis, A., Tychowski, M., Kalantzopoulos, G. et al. Studies on bacteriocin (thermophilin T) production by Streptococcus thermophilus ACA-DC 0040 in batch and fed-batch fermentation modes. Antonie van Leeuwenhoek 92, 207–220 (2007). https://doi.org/10.1007/s10482-007-9148-4

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  • DOI: https://doi.org/10.1007/s10482-007-9148-4

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