Abstract
Double-stranded RNA viruses of about 35 nm in diameter were isolated from a mycocin-secreting strain of Cryptococcus aquaticus. A derivative of this strain, lacking small dsRNA, was non-mycocinogenic and sensitive to its own toxin. The killing pattern of this mycocin was restricted to some species of the Cystofilobasidiales clade. Despite the differences in genome size of dsRNA viruses in mycocinogenic strains of Cryptococcus aquaticus, Cystofilobasidium sp. CBS 6569, Cystofilobasidium bisporidii, Cystofilobasidium infirmominiatum, Trichosporon pullulans and Xanthophyllomyces dendrorhous and killing patterns of their mycocins, the viral genomes showed homology in hybridisation experiments.
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References
Altschul S.F., Madden T.L., Schaer A.A., Zhang J., Zhang Z.,Miller W. and Lipman D.J. 1997. Gapped BLAST andPSI-BLAST: a new generation of protein database search pro-grams. Nucl. Acid Res. 25: 3389–3402.
Bab'eva I.P., Golubev W.I., Reshetova I.S., Azieva E.E. and Bla-godatskayaV.M. 1976. Yeasts in high-latitude regions of thenorthern and southern hemispheres. Vestn. Mosk. Univ. _ser. biol., pochvoved. 2: 81–87.
Barnett J.A., Payne R.W. and Yarrow D. 2000. Yeasts: Character-isticsand Identification, 3 rd edn Cambridge Univ. Press, Cambridge,UK.
Bevan E.A. and Makover M. 1963. The physiological basis of thekiller-character in yeast. Proc. Int. Congr. Genet. 1: 202–203.
Boekhout T., Poot G., Hackman P. and Steensma H.Y. 1991. Ge-nomiccharacterization of strains of Itersonilia: taxonomic con-sequencesand life cycle. Can. J. Microbiol. 37: 188–194.
Castillo A. and Cifuentes V. 1994. Presence of double-strandedRNA and virus-like particles in Phaffýa rhodozyma. Curr. Genet. 26: 364–368.
Diaz M.R. and Fell J.W. 2000. Molecular analyses of the IGS & ITS regions of rDNA of the psychrophilic yeasts in the genusMrakia. Ant. van Leeuwenhoek 77: 7–12.
Fell J.W., Boekhout T., Fonseca A., Scorzetti G. and Statzell-Tall-manA. 2000. Biodiversity and systematics of basidiomycetousyeasts as determined by large-subunit rDNA D1/D2 domain se-quenceanalysis. Int. J. Syst. Evol. Microbiol. 50: 1351–1371.
Gácser A., Hamari Z., Pfeiffer I., Varga J., Kevei F. and Kucsera J. 2001. Genetic diversity in the red yeast Cryptococcus hungari-cusand its phylogenetic relationship to some basidiomycetousyeasts. FEMS Yeast Res. 1: 213–220.
Golubev W.I. 1990. Action spectrum of Cystofilobasidium bispori-diimycocin from a taxonomic viewpoint. Mykol. Phytopathol. 24: 542–547.
Golubev W.I. 1998. Mycocins (Killer toxins). In: Kurtzman C.P. and Fell J.W. (eds), The yeasts. A taxonomic study, 4 th edn.Elsevier, Amsterdam, The Netherlands, pp. 55–62.
Golubev W.I., Pfeiffer I. and Golubeva A.W. 2002. Mycocin pro-ductionin Trichosporon pullulans populations colonizing treeexudates in the spring. FEMS Microbiol. Ecol. 40: 151–157.
Golubev W.I., Pfeiffer I., Churkina L.G. and Golubeva A.W. 2003. Double-stranded RNA viruses in a mycocinogenic strain ofCystofilobasidium infirmominiatum. FEMS Yeast Res. 3: 63–68.
Karamysheva Z.N., Ksenzenko V.N., Golubev W.I., Ratner E.N. and Tikhomirova L.P. 1993. Characterisation of viruses in my-cocinogenicstrain VKM Y-2700 of Cystofilobasidium bisporidii. Dokl. Russ. Acad. Sci. 331: 376–378.
Kurtzman C.P. and Fell J.W., (eds) 1998. The Yeasts: A Taxonomic Study. 4th edn. Elsevier Sci., Amsterdam, The Netherland
Leach J., Finkelstein D.B. and Rambosek J.A. 1986. Rapid mini-prepof DNA from filamentous fungi. Fungal Gen. Newslett. 33: 32–33.
Oliver S.G., McCready S.J., Holm C., Sutherland P.A., McLaugh-linC.S. and Cox B.S. 1977. Biochemical and physiologicalstudies of the yeast virus-like particle. J. Bact. 130: 1303–1309.
Pfeiffer I., Kucsera J., Varga J., Párducz Á. and Ferenczy L. 1996.Variability and inheritance of double-stranded RNA viruses inPhaffýa rhodozyma. Curr. Genet. 30: 294–297.
Pfeiffer I., Gyantár M., Kucsera J. and Párducz Á. 1998. Isolationof dsRNA-associated VLPs from the strain Cryptococcus hun-garicusCBS 6569. FEMS Microbiol. Lett. 162: 151–154.
Scorzetti G., Fell J.W., Fonseca A. and Statzell-Tallman A. 2002. Systematics of basidiomycetous yeasts: a comparison of largesubunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res. 2: 495–517.
Yarrow D. 1998. Methods for the isolation, maintenance and iden-tificationof yeasts. In: Kurtzman C.P. and Fell J.W. (eds), TheYeasts: a Taxonomic Study, 4 th edn. Elsevier, Amsterdam, TheNetherlands, pp. 77–100.
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Pfeiffer, I., Golubev, W.I., Farkas, Z. et al. Mycocin production in Cryptococcus aquaticus . Antonie Van Leeuwenhoek 86, 369–375 (2004). https://doi.org/10.1007/s10482-004-0888-0
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DOI: https://doi.org/10.1007/s10482-004-0888-0