Skip to main content
SpringerLink
Log in

Four distinct isolates of a novel polymycovirus identified in Setosphaeria turcica

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Isolation and analysis of double-stranded RNA (dsRNA) from the phytopathogenic fungus Setosphaeria turcica f. sp. zeae revealed the presence of a new double-stranded RNA (dsRNA) virus, tentatively named "Setosphaeria turcica polymycovirus 2" (StPmV2). The genome of StPmV2 consists of five segments (dsRNA1-5), ranging in size from 965 bp to 2462 bp. Each dsRNA contains one open reading frame (ORF) flanked by 5' and 3' untranslated regions (UTRs) with conserved terminal sequences. The putative protein encoded by dsRNA1 shows 64.52% amino acid sequence identity to the RNA-dependent RNA polymerase (RdRp) of the most closely related virus, Cladosporium cladosporioides virus 1, which belongs to the family Polymycoviridae. dsRNAs 2-4 encode the putative coat protein, methyltransferase (MTR), and proline-alanine-serine-rich protein (PASrp), respectively, and dsRNA5 encodes a protein of unknown function. Phylogenetic analysis based on the RdRp protein indicated that StPmV2 clustered with members of the family Polymycoviridae and is therefore a new mycovirus belonging to the genus Polymycovirus in the family Polymycoviridae. In addition, three other distinct isolates of StPmV2 were identified: one isolated from S. turcica f. sp. zeae and two from S. turcica f. sp. sorghi. To our knowledge, this is the first report of a polymycovirus infecting both S. turcica f. sp. zeae and S. turcica f. sp. sorghi.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

Data availability

Not available.

References

  1. Kotta-Loizou I, Coutts RH (2017) Studies on the virome of the entomopathogenic fungus Beauveria bassiana reveal novel dsRNA elements and mild hypervirulence. PLoS Pathog 13(1):e1006183

    Article  PubMed  PubMed Central  Google Scholar 

  2. Sato Y, Jamal A, Kondo H, Suzuki N (2020) Molecular characterization of a novel polymycovirus from Penicillium janthinellum with a focus on its genome-associated PASrp. Front Microbiol 20(11):592789

    Article  Google Scholar 

  3. Filippou C, Diss RM, Daudu JO, Coutts RHA, Kotta-Loizou I (2021) The polymycovirus-mediated growth enhancement of the entomopathogenic fungus Beauveria bassiana is dependent on Carbon and Nitrogen metabolism. Front Microbiol 12:606366

    Article  PubMed  PubMed Central  Google Scholar 

  4. Kang Q, Li L, Li J, Zhang S, Xie J, Li Q, Zhang Z (2021) A novel polymycovirus with defective RNA isolated from the entomopathogenic fungus Beauveria bassiana Vuillemin. Arch Virol 166(12):3487–3492

    Article  CAS  PubMed  Google Scholar 

  5. Mahillon M, Decroës A, Liénard C, Bragard C, Legrève A (2019) Full genome sequence of a new polymycovirus infecting Fusarium redolens. Arch Virol 164(8):2215–2219

    Article  CAS  PubMed  Google Scholar 

  6. Ma G, Wu C, Li Y, Mi Y, Zhou T, Zhao C, Wu X (2022) Identification and genomic characterization of a novel polymycovirus from Alternaria alternata causing watermelon leaf blight. Arch Virol 167(1):223–227

    Article  CAS  PubMed  Google Scholar 

  7. Cao J, Xie F, Zhang Z, Zhu H, Zhou X (2022) Molecular characterization of a novel polymycovirus identified in the phytopathogenic fungus Colletotrichum gloeosporioides. Arch Virol 167(12):2805–2810

    Article  CAS  PubMed  Google Scholar 

  8. Singh P, Huang SY, Hernandez AG, Adhikari P, Jamann TM, Mideros SX (2022) Genomic regions associated with virulence in Setosphaeria turcica identified by linkage mapping in a biparental population. Fungal Genet Biol 159:103655

    Article  CAS  PubMed  Google Scholar 

  9. Mueller DS, Wise KA, Sisson AJ, Allen TW, Bergstrom GC, Bissonnette KM, Bradley CA, Byamukama E, Chilvers MI, Collins AA, Esker PD (2020) Corn yield loss estimates due to diseases in the United States and Ontario, Canada, from 2016 to 2019. Plant Health Prog 21:238–247

    Article  Google Scholar 

  10. Gao Z, Zhang M, Yu T, Wang X, Wang X, An H, Zhang S, Liu M, Fang S (2021) Molecular characterization of a novel polymycovirus from the phytopathogenic fungus Setosphaeria turcica. Arch Virol 166:2315–2319

    Article  CAS  PubMed  Google Scholar 

  11. Wang H, Li C, Cai L, Fang S, Zheng L, Yan F, Zhang S, Liu Y (2018) The complete genomic sequence of a novel botybirnavirus isolated from a phytopathogenic Bipolaris maydis. Virus Genes 54:733–736

    Article  CAS  PubMed  Google Scholar 

  12. Wu R, Yang Y, Duan X, An H, Du Z, Zhang S, Zhang X (2020) Four distinct isolates of Helminthosporium victoriae virus 190S identified from Bipolaris maydis. Virus Res 285:197941

    Article  CAS  PubMed  Google Scholar 

  13. Potgieter AC, Page NA, Liebenberg J, Wright IM, Landt O, van Dijk AA (2009) Improved strategies for sequence-independent amplification and sequencing of viral double-stranded RNA genomes. J Gen Virol 90:1423–1432

    Article  CAS  PubMed  Google Scholar 

  14. Kotta-Loizou I, Coutts RHA, Ictv Report Consortium (2022) ICTV virus taxonomy profile: Polymycoviridae 2022. J Gen Virol 103(5). https://doi.org/10.1099/jgv.0.001747

  15. Hu G, Kurgan L (2019) Sequence similarity searching. Curr Protoc Protein Sci 95:e71

    Article  PubMed  Google Scholar 

  16. Poch O, Blumberg BM, Bougueleret L, Tordo N (1990) Sequence comparison of five polymerases (L proteins) of unsegmented negative-strand RNA viruses: theoretical assignment of functional domains. J Gen Virol 71:1153–1162

    Article  CAS  PubMed  Google Scholar 

  17. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–1874

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Sato Y, Shamsi W, Jamal A, Bhatti MF, Kondo H, Suzuki N (2020) Hadaka virus 1: a capsidless eleven-segmented positive sense single-stranded RNA virus from a phytopathogenic fungus, Fusarium oxysporum. MBio 11:e00450-e1004

    Article  PubMed  PubMed Central  Google Scholar 

  19. Kanematsu S, Shimizu L, Salaipeth H, Yaegashi Y, Sasaki T, Ito T, Suzuki N (2014) Genome rearrangement of a mycovirus Rosellinia necatrix megabirnavirus 1 affecting its ability to attenuate virulence of the host fungus. Virology 451:308–315

    Article  Google Scholar 

  20. Zhai L, Xiang J, Zhang M, Fu M, Yang Z, Hong N, Wang G (2016) Characterization of a novel double-stranded RNA mycovirus conferring hypovirulence from the phytopathogenic fungus Botryosphaeria dothidea. Virology 493:75–85

    Article  CAS  PubMed  Google Scholar 

  21. Kanhayuwa L, Kotta-Loizou I, Ozkan S, Gunning AP, Coutts RHA (2015) A novel mycovirus from Aspergillus fumigatus contains four unique dsRNAs as its genome and is infectious as dsRNA. Proc Natl Acad Sci USA 112:9100–9105

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Jia H, Dong K, Zhou L, Wang G, Hong N, Jiang D, Xu W (2017) A dsRNA virus with filamentous viral particles. Nat Commun 8(1):168

    Article  PubMed  PubMed Central  Google Scholar 

  23. Zoll J, Verweij PE, Melchers WJG (2018) Discovery and characterization of novel Aspergillus fumigatus mycoviruses. PLoS ONE 13:e0200511

    Article  PubMed  PubMed Central  Google Scholar 

  24. Muhire BM, Varsani A, Martin DP (2014) SDT: a virus classification tool based on pairwise sequence alignment and identity calculation. PLoS ONE 26(9):e108277

    Article  Google Scholar 

  25. Ghabrial SA, Nibert ML (2009) Victorivirus, a new genus of fungal viruses in the family Totiviridae. Arch Virol 154:373–379

    Article  CAS  PubMed  Google Scholar 

  26. Niu Y, Yuan Y, Mao J, Yang Z, Cao Q, Zhang T, Wang S, Liu D (2018) Characterization of two novel mycoviruses from Penicillium digitatum and the related fungicide resistance analysis. Sci Rep 8(1):5513

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Funding

This work was supported by the Science and Technology Project of the Anshun Branch of Guizhou Tobacco Company (no. 2022520400140040) and the National Natural Science Foundation of China (no. 31972243).

Author information

Authors and Affiliations

  1. MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province), Hubei Engineering Research Center for Pest Forewarning and Management, Yangtze University, Jingzhou, 434025, China

    Yun Zheng, Miaomiao Chen, Zhongnan Gao, Qingchao Deng, Shouguo Fang & Songbai Zhang

  2. Anshun Branch of Guizhou Tobacco Company, Anshun, 561000, China

    Xiquan Li, Fei Dai & Shouhui Pan

Authors
  1. Yun Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Miaomiao Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiquan Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fei Dai
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zhongnan Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Qingchao Deng
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Shouguo Fang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Songbai Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Shouhui Pan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Songbai Zhang or Shouhui Pan.

Ethics declarations

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Handling Editor: Robert H.A. Coutts.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zheng, Y., Chen, M., Li, X. et al. Four distinct isolates of a novel polymycovirus identified in Setosphaeria turcica. Arch Virol 168, 189 (2023). https://doi.org/10.1007/s00705-023-05819-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00705-023-05819-1

Search

Navigation