Abstract
We developed a novel flow particle analyzer that automatically classifies airborne pollen grains. The design of the particle counter (model KP-1000) is based on that of a flow cytometer, applied to the measurement of airborne particles. The counter classifies pollen species by simultaneously detecting both scattered light and the characteristic fluorescence excited by ultraviolet light in the flow cell. We observed airborne pollen using KP-1000 pollen counters and Durham samplers to compare their performance at three study sites in Japan during the spring pollen season. The pollen counter followed the variation in pollen concentrations, and its daily pollen counts were significantly correlated with the results of the Durham sampling method at all study sites. Although the counter over- or under-counted 2 target pollen species (Cryptomeria japonica and Chamaecyparis obtusa) when they coexisted, a data correction based on the Durham sampling results improved the accuracy of pollen classification of the counter. Our results indicate that the new pollen counter has a strong potential for counting and identifying airborne pollen grains in real time, and it requires further improvement, field trials, and tests with other common airborne pollen grains.
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References
Araki, H. (1961). Studies on pollinosis: II. Sensitization with pollens in Japan (in Japanese). Japanese Journal of Allergology, 10(6), 354–370.
Conner, W. D. (1966). An inertial-type particle separator for collecting large samples. Journal of Air pollution Control Association, 16, 35–38.
Delaunay, J. J., Seymour, C., & Feuillet, V. (2004). Investigation of short-range cedar pollen forecasting. Physical Review E, 70, 066214.
Durham, O. C. (1946). The volumetric incidence of atmospheric allergens IV. A proposed standard method of gravity sampling, counting, and volumetric interpolation of results. Journal of Allergy, 17, 79–86.
Enokido, H., UGWU, C. U., Mitsumoto, K., Yabusaki, K., & Aoyagi, H. (2008). Comparative analysis of autofluorescence and diameter for the spherical pollen of Cryptomeria japonica and Chamaecyparis obtusa pollen in Japan (in Japanese). Japanese Journal of Palynology, 54(2), 69–77.
Fujimura, T., Futamura, N., Midoro-Horiuti, T., Togawa, A., Goldblum, R. M., Yasueda, H., et al. (2007). Isolation and characterization of native Cry j 3 from Japanese cedar (Cryptomeria japonica) pollen. Allergy, 62(5), 547–553.
Harison, P. P., Ho, J., & Quant, F. R. (1997). Design of an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence. Journal of Aerosol Science, 28(3), 471–482.
Hashimoto, M., Nigi, H., Sakaguchi, M., Inouye, S., Imaoka, K., Miyazawa, H., et al. (1995). Sensitivity to two major allergens (Cry j I and Cry j II) in patients with Japanese cedar (Cryptomeria japonica) pollinosis. Clinical & Experimental Allergy, 25(9), 848–852.
Hirst, J. (1952). An automatic volumetric spore trap. Annals of Applied Biology., 39(2), 257–265.
Horiguchi, S., & Saito, Y. (1964). Japanese cedar pollinosis in Nikko, Japan (in Japanese). Japanese Journal of Allergology, 13(1), 16–18.
Ide, T., Yamamoto, K., Tabata, S., & Ashida, T. (1995). N-terminal amino acid sequence of a major allergen, Cha o 1, of Japanese cypress pollen (in Japanese). Japanese Journal of Palynology, 41, 69–72.
Ito, H., Nishimura, J., Suzuki, M., Mamiya, S., Sato, K., Takagi, I., et al. (1995). Specific IgE to Japanese cypress (Chamaecyparis obtusa) in patients with nasal allergy. Annals of Allergy, Asthma and Immunology, 74(4), 299–303.
Kaye, P. H., Brton, J. E., Hirst, E., & Clark, J. M. (2000). Simultaneous light scattering and intrinsic fluorescence measurement for the classification of airborne particles. Applied Optics, 39(21), 3738–3745.
McCrone, W. C., & Delly, J. G. (1973). Ultraviolet (365 nm) and blue–violet fluorescence analysis of particles. In W. C. McCrone, J. C. Delly & S. J. Palenik (Eds.), The Particle Atlas (Vol. IV, p. 857). Ann Arbor: Ann Arbor Science Publishers.
Mitsumoto, K., Yabusaki, K., & Aoyagi, H. (2009). Classification of pollen species using autofluorescence image analysis. Journal of Bioscience and Bioengineering, 107(1), 90–94.
Mori, T., Yokoyama, M., Komiyama, N., Okano, M., & Kino, K. (1999). Purification, identification, and cDNA cloning of Cha o 2, the second major allergen of Japanese cypress pollen. Biochemical and Biophysical Research Communications, 263(1), 166–171.
Nishihata, S., Inouye, S., Saiga, T., Sahashi, N., Suzuki, S., Murayama, K., et al. (1999). Prevalence rate of allergy to Japanese cedar pollen in Tokyo: From field investigation in 1996 by Tokyo Japanese Cedar Pollen Allergy Measurements and Review Committee (in Japanese). Japanese Journal of Allergy, 48(6), 597–604.
Ohno, N., Ide, T., Sakaguchi, M., Inouye, S., & Saito, S. (2000). Common antigenicity between Japanese cedar (Cryptomeria japonica) pollen and Japanese cypress (Chamaecyparis obtusa) pollen, II. Determination of the cross-reacting T-cell epitope of Cry j 1 and Cha o 1 in mice. Immunology, 99, 630–634.
Pan, Y. L., Hartings, J., Pinnick, R. G., Hill, S. C., Halverson, J., & Chang, R. K. (2003). Single-particle fluorescence spectrometer for ambient aerosols. Aerosol Science and Technology, 37, 627–638.
Pinnick, R. G., Hill, S. C., Nachman, P., Pendleton, J. D., Fernandez, G. L., Mayo, M. W., et al. (1995). Fluorescence particle counter for detecting airborne bacteria and other biological particles. Aerosol Science and Technology, 23, 653–664.
Sakaguchi, M., Inouye, S., Taniai, M., Ando, S., Usui, M., & Matuhasi, T. (1990). Identification of the second major allergen of Japanese cedar pollen. Allergy, 45, 309–312.
Sugita, K., & Furuya, K. (1964). Studies on pollinosis: I. Short ragweed and orchard grass (in Japanese). Japanese Journal of Allergology, 13(1–2), 19–23.
Suzuki, M., Komiyama, N., Itoh, M., Itoh, H., Sone, T., Kino, K., et al. (1996). Purification, characterization and molecular cloning of Cha o 1, a major allergen of Chamaecyparis obtusa (Japanese cypress) pollen. Molecular Immunology, 33(4–5), 451–460.
Suzuki, M., Tonouchi, M., Murayama, K., & Mitsumoto, K. (2005). An analysis of high concentration of pollen at early morning in 2005 spring and a measurement of Japanese cedar and cypress pollen using auto-fluorescence characteristics (in Japanese). Journal of Aerosol Research, 20(4), 281–289.
Wagatsuma, Y., Shida, T., Matsuyama, R., Miyata, M., & Ito, K. (1969). Ezo-Yomogi (Artemisia Montana) pollinosis in Sapporo, Hokkaido, Japan (in Japanese). Japanese Journal of Allergology, 18(12), 980–990.
Yasueda, H., Yui, Y., Shimizu, T., & Shida, T. (1983). Isolation and partial characterization of the major allergen from Japanese cedar (Cryptomeria japonica) pollen. Journal of Allergy and clinical immunology, 71(1), 77–86.
Acknowledgments
The KP-1000 data were provided by the Ministry of the Environment, Government of Japan. The Durham sampler pollen data were provided by the Bureau of Social Welfare and Public Health of the Tokyo Metropolitan Government. The author is grateful to Koji Murayama, Motoo Suzuki and Michihiko Tonouchi, JMBSC, for giving adequate advice, and Dr. Muneharu Ishikawa and Dr. Yoshiaki Shirasawa for providing the opportunity to report the results of this study.
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Mitsumoto, K., Yabusaki, K., Kobayashi, K. et al. Development of a novel real-time pollen-sorting counter using species-specific pollen autofluorescence. Aerobiologia 26, 99–111 (2010). https://doi.org/10.1007/s10453-009-9147-1
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DOI: https://doi.org/10.1007/s10453-009-9147-1