Abstract
This work aims to investigate the presence of airborne grass pollen and to identify antigenic and allergenic profiles from eight different grass species collected in the Porto region (Portugal). Poaceae airborne pollen, sampled using a Hirst-type volumetric trap during 2003–2007, was the second most abundant type, and high concentrations were found from April to August. Pollen proteins extracted from the eight grass species collected were separated by SDS-PAGE, being the allergenic profile investigated by immunoblotting using sera from atopic patients and maize profilin polyclonal antibody (ZmPRO3). Pollen extract profiles showed several bands ranging from 10 to 97 kDa. In immunoblotting studies, a low molecular weight protein (12–13 kDa) was recognized by profilin antibody. Also, in all pollen extracts except Zea mays, the IgE binding proteins of 12–13 kDa were detected in sera from the 25 patients with different sensitization profiles presenting high IgE values (>80 kU/l). This protein can be considered as a potential causal agent of the allergic respiratory diseases.
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Andersson, K., & Lidholm, J. (2003). Characteristics and immunobiology of grass pollen allergens. International Archives of Allergy and Immunology, 130(2), 87–107.
Asero, R., Mistrello, G., Roncarolo, D., & Amato, S. (2004). Parietaria profilin shows only limited cross-reactivity with birch and grass profilins. International Archives of Allergy and Immunology, 133(2), 121–124.
Barderas, R., Villalba, M., Pascual, C. Y., Batanero, E., & Rodriguez, R. (2004). Profilin (Che a 2) and polcalcin (Che a 3) are relevant allergens of Chenopodium album pollen: Isolation, amino acid sequences, and immunologic properties. Journal of Allergy and Clinical Immunology, 113(6), 1192–1198.
Bilisik, A., Yenigun, A., Bicakci, A., Eliacik, K., Canitez, Y., Malyer, H., et al. (2008). An observation study of airborne pollen fall in Didim (SW Turkey): Years 2004–2005. Aerobiologia, 24(1), 61–66.
Bradford, M. (1976). A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248–254.
Christensen, H. E. M., Ramachandran, S., Tan, C. T., Surana, U., Dong, C. H., & Chua, N. H. (1996). Arabidopsis profilins are functionally similar to yeast profilins: Identification of a vascular bundle-specific profilin and a pollen-specific profilin. Plant Journal, 10(2), 269–279.
Fernandez, J., Asturias, J. A., & Martinez, A. (2000). Biological standardization and allergen characterization of date palm pollen (Phoenix dactylifera). Allergy, 55(S63), 140.
Ford, S. A., & Baldo, B. A. (1986). A reexamination of ryegrass (Lolium-Perenne) pollen allergens. International Archives of Allergy and Applied Immunology, 81(3), 193–203.
García-Mozo, H., Pérez-Badia, R., Fernández-González, F., & Galán, C. (2006). Airborne pollen sampling in Toledo, Central Spain. Aerobiologia, 22(1), 55–66.
Gibbon, B. C., Ren, H. Y., & Staiger, C. J. (1997). Characterization of maize (Zea mays) pollen profilin function in vitro and in live cells. Biochemical Journal, 327, 909–915.
Hrabina, M., Peltre, G., Van Ree, R., & Moingeon, P. (2008). Grass pollen allergens. Clinical & Experimental Allergy Reviews, 8(1), 7–11.
Huang, S. R., McDowell, J. M., Weise, M. J., & Meagher, R. B. (1996). The Arabidopsis profilin gene family—Evidence for an ancient split between constitutive and pollen-specific profilin genes. Plant Physiology, 111(1), 115–126.
Jockusch, B. M., Murk, K., & Rothkegel, M. (2007). The profile of profilins. Reviews of Physiology, Biochemistry and Pharmacology, 159, 131–149.
Karakesisoglou, I., Schleicher, M., Gibbon, B. C., & Staiger, C. J. (1996). Plant profilins rescue the aberrant phenotype of profilin-deficient Dictyostelium cells. Cell Motility and the Cytoskeleton, 34(1), 36–47.
Knox, B., & Suphioglu, C. (1996). Environmental and molecular biology of pollen allergens. Trends in Plant Science, 1(5), 156–164.
Laemmli, U. K. (1970). Cleavage of structural proteins during assembly of head of bacteriophage-T4. Nature, 227(5259), 680–685.
Matthiesen, F., Schumacher, M. J., & Lowenstein, H. (1989). An immunoelectrophoretic analysis of the allergens of Cynodon-Dactylon (Bermuda Grass) Pollen. Journal of Allergy and Clinical Immunology, 83(6), 1124–1134.
Mohapatra, S. S., Lockey, R. F., & Shirley, S. (2005). Immunobiology of grass pollen allergens. Current Allergy and Asthma Reports, 5(5), 381–387.
Murray, M., Scoffield, R., Galán, C., & Villamil, C. (2007). Airborne pollen sampling in a wildlife reserve in the south of Buenos Aires province, Argentina. Aerobiologia, 23(2), 107–117.
Nitiu, D. (2006). Aeropalynologic analysis of La Plata City (Argentina) during a 3-year period. Aerobiologia, 22(1), 79–87.
Reindl, J., Rihs, H. P., Scheurer, S., Wangorsch, A., Haustein, D., & Vieths, S. (2002). IgE reactivity to profilin in pollen-sensitized subjects with adverse reactions to banana and pineapple. International Archives of Allergy and Immunology, 128(2), 105–114.
Ribeiro, H., Cunha, M., & Abreu, I. (2005). Aeropalynological study of Vitis vinifera in the Braga region (1999–2003). Aerobiologia, 21, 131–138.
Ribeiro, H., Cunha, M., & Abreu, I. (2007). Definition of the main pollen season using a logistic model. Annals of Agricultural and Environmental Medicine, 14, 159–167.
Sridhara, S., Kumar, L., Verma, J., Arora, N., Gaur, S. N., & Singh, B. P. (2002). Immunobiochemical characterization of Sorghum vulgare pollen allergens prevalent in tropical countries. Indian Journal of Allergy Asthma and Immunology, 16, 33–39.
Vallverdu, A., GarciaOrtega, P., Martinez, J., Martinez, A., Esteban, M. I., deMolina, M., et al. (1997). Mercurialis annua: Characterization of main allergens and cross-reactivity with other species. International Archives of Allergy and Immunology, 112(4), 356–364.
Van Ree, R., Driessen, M. N. B. M., Leeuwen, W. A., Stapel, S. O., & Aalberse, R. C. (1992). Variability of crossreactivity of IgE antibodies to group I and V allergens in eight grass pollen species. Clinical & Experimental Allergy, 22(6), 611–617.
Weber, R. W. (2007). Cross-reactivity of pollen allergens: Impact on allergen immunotherapy. Annals of Allergy Asthma & Immunology, 99(3), 203–212.
Witke, W., Podtelejnikov, A. V., Di Nardo, A., Sutherland, J. D., Gurniak, C. B., Dotti, C., et al. (1998). In mouse brain profilin I and profilin II associate with regulators of the endocytic pathway and actin assembly. Embo Journal, 17(4), 967–976.
Acknowledgments
We are grateful to Prof. Chris J. Staiger (Department of Biological Sciences, Purdue University) for kindly providing the polyclonal rabbit anti-ZmPRO3 serum and for the critical reading and suggestions added to this manuscript. This work was supported by Fundação Calouste Gulbenkian Project 77161: Bioaerosol, Non-Biological Polutants and Public Health.
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Abreu, I., Ribeiro, N., Ribeiro, H. et al. Airborne Poaceae pollen in Porto (Portugal) and allergenic profiles of several grass pollen types. Aerobiologia 24, 133–140 (2008). https://doi.org/10.1007/s10453-008-9093-3
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DOI: https://doi.org/10.1007/s10453-008-9093-3