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Type I Collagen-Based Fibrous Capsule Enhances Integration of Tissue-Engineered Cartilage with Native Articular Cartilage

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Abstract

Successful integration of engineered constructs with host tissues is crucial for cartilage repair, yet achieving it remains challenging. A collagen I-based fibrous capsule characterized by increased cell density and decreased glycosaminoglycan deposition usually forms at the periphery of tissue-engineered cartilage. The current study aimed to evaluate the effects of a solid fibrous capsule on construct integration with native articular cartilage. To this end, capsule-containing (CC) and capsule-free (CF) constructs were grown by culturing chondrocyte-seeded scaffolds with insulin-like growth factor-1 and transforming growth factor-β1, respectively, in a wavy-walled bioreactor that imparts hydrodynamic forces for 4 weeks. The ability of harvested constructs to integrate with native cartilage was determined using a cartilage explant model. Our results revealed that adhesive stress between native cartilage and the CC constructs was 57% higher than that in the CF group, potentially due to the absence of glycosaminoglycans and increased cell density in the capsule region and deposition of denser and thicker collagen fibrils at the integration site. The present work demonstrates that the fibrous capsule can effectively enhance early integration of engineered and native cartilage tissues and thus suggests the need to include the capsule as a variable in the development of cartilage tissue engineering strategies.

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Acknowledgments

This work was supported by the National Science Foundation [NSF0602608].

Conflict of interest

No competing financial interests exist.

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Authors and Affiliations

  1. The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, 30332, USA

    Yueh-Hsun Yang & Gilda A. Barabino

  2. Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA, 30602, USA

    Mary B. Ard & Jaroslava T. Halper

  3. The Grove School of Engineering, The City College of New York, Steinman Hall T-142, 160 Convent Avenue, New York, NY, 10031, USA

    Gilda A. Barabino

Authors
  1. Yueh-Hsun Yang
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  2. Mary B. Ard
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  3. Jaroslava T. Halper
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  4. Gilda A. Barabino
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Corresponding author

Correspondence to Gilda A. Barabino.

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Associate Editor Michael S. Detamore oversaw the review of this article.

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Yang, YH., Ard, M.B., Halper, J.T. et al. Type I Collagen-Based Fibrous Capsule Enhances Integration of Tissue-Engineered Cartilage with Native Articular Cartilage. Ann Biomed Eng 42, 716–726 (2014). https://doi.org/10.1007/s10439-013-0958-4

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  • DOI: https://doi.org/10.1007/s10439-013-0958-4

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