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Differential Effect of Scaffold Shape on Dentin Regeneration

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Abstract

The effect of scaffold shape on dentin regeneration is not well understood. In this study, porous hydroxyapatite/beta-tricalcium phosphate (HAp/β-TCP), powdered HAp/β-TCP, and polyglycolic acid (PGA) fiber mesh were used as scaffolds and transplanted with cultured porcine dental pulp-derived cells into the backs of nude mice. Samples were harvested after 6 weeks. Newly-formed hard tissue was observed in all transplants. When porous HAp/β-TCP was used, dentin-like hard tissue was observed on the inner wall with minimum cell inclusions and odontoblast-like cells were aligned adjacent to the hard tissue. When HAp/β-TCP powders or PGA were used, bone-like hard tissues showed cell inclusions and cell alignment was not observed. Hard tissue from the HAp/β-TCP block group was positive for type I collagen, osteonectin, bone sialoprotein and dentin sialoprotein (DSP), which are markers for dentin. This result was confirmed by in situ hybridization with a dsp probe. Only the aligned cells were positive with an antisense probe. On the other hand, hard tissue from other scaffolds showed incomplete expression of both bone and dentin markers and they were negative for osteonectin and DSP. These results suggest that scaffold shape affects the type of tissue regenerated by dental pulp-derived cells.

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Acknowledgments

We thank to Kimie Mase and Sumiko Yabe for technical support for in situ hybridization analyses.

Author information

Author notes

  1. Hideaki Kagami

    Present address: Division of Molecular Therapy, The Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan

Authors and Affiliations

  1. Research & Development Center, Hitachi Medical Corporation, Kashiwa, Japan

    Akiko Tonomura & Yusuke Ando

  2. Department of Tissue Engineering, Nagoya University School of Medicine, Nagoya, Japan

    Akiko Tonomura, Yoshinori Sumita & Hideaki Kagami

  3. Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan

    Daiki Mizuno, Yusuke Ando, Yoshinori Sumita & Minoru Ueda

  4. Advanced Research Laboratory, Hitachi Ltd., Hatoyama, Saitama, Japan

    Akiko Hisada & Norihito Kuno

  5. Department of Regenerative Oral Surgery, Graduate School of Biomedical Science, Nagasaki University, Nagasaki, Japan

    Yoshinori Sumita

  6. Division of Stem Cell Engineering, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan

    Masaki J. Honda, Minoru Ueda & Hideaki Kagami

  7. Department of Anatomy, Nihon University School of Dentistry, Tokyo, Japan

    Masaki J. Honda

  8. Second Department of Oral and Maxillofacial Surgery, School of Dental Medicine, Tsurumi University, Yokohama, Japan

    Kazuhito Satomura

  9. Sakurai Dental Clinic., Kobe, Japan

    Hiroya Sakurai

Authors
  1. Akiko Tonomura
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  2. Daiki Mizuno
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  3. Akiko Hisada
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  4. Norihito Kuno
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  5. Yusuke Ando
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  6. Yoshinori Sumita
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  7. Masaki J. Honda
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  8. Kazuhito Satomura
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  9. Hiroya Sakurai
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  10. Minoru Ueda
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  11. Hideaki Kagami
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Corresponding author

Correspondence to Hideaki Kagami.

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Associate Editor Jennifer West oversaw the review of this article.

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Tonomura, A., Mizuno, D., Hisada, A. et al. Differential Effect of Scaffold Shape on Dentin Regeneration. Ann Biomed Eng 38, 1664–1671 (2010). https://doi.org/10.1007/s10439-010-9910-z

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  • DOI: https://doi.org/10.1007/s10439-010-9910-z

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