Abstract
Purpose
To study the diastolic functions using color Doppler M-mode (CDMM) for noninvasive analysis of the intraventricular pressure difference (IVPD) in diabetic rats.
Methods
Two equal groups of rats were included: control and streptozotocin-induced DM (n = 15). The cardiac functions were examined monthly using conventional echocardiography and CDMM with a specific MATLAB software. Echocardiography was performed under 2% isoflurane mask inhalation. Five months thereafter, all rats were killed for macroscopic and microscopic examinations of the cardiac fibrosis.
Results
DM rats showed higher systolic blood pressure and diastolic dysfunction, i.e., decreases in several parameters such as E, E/A, TDIs, and IVPDs, compared to the controls. Moreover, obvious cardiac fibrosis was seen in perivascular and interstitial tissues, but there were no notable differences in terms of gross lesions.
Conclusions
Because of the noninvasive nature of CDMM, IVPD and other conventional echocardiographic parameters can be used as reliable indicators generally for evaluating cardiac function and particularly the change in intraventricular pressure.
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Acknowledgements
We would like to thank the toxicology laboratory members for sectioning and staining tissue, and the members of Juntendo University for analyzing the IVPD.
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All procedures and protocols used in this experiment were performed and approved in accordance with the standard guidelines described in “Guide for the Care and Use of Laboratory Animals” of Tokyo University of Agriculture and Technology (Approval number: 29-79).
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Kitpipatkun, P., Matsuura, K., Shimada, K. et al. Key factors of diastolic dysfunction and abnormal left ventricular relaxation in diabetic rats. J Med Ultrasonics 47, 347–356 (2020). https://doi.org/10.1007/s10396-020-01021-x
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DOI: https://doi.org/10.1007/s10396-020-01021-x