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Interactive Effect of Tocopherol, Salicylic Acid and Ascorbic Acid on Agronomic Characters of Two Genotypes of Brassica napus L. Under Induced Drought and Salinity Stresses

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Abstract

Several agricultural systems are vulnerable to the harmful effects of climate change in today’s increasingly vulnerable world. Drought and salinity are two major factors in climate change that can severely slow down the development of many crops. This study determined the influence of induced drought and salinity on growth of two varieties of Brassica napus L. (Shiralee and Vanguard), after priming with salicylic acid, ascorbic acid and tocopherol. Seed priming significantly promoted seed germination and reduced the normal germination time of seeds under both induced stressors. Significant increase in shoot length, shoot and root fresh weight, dry shoot weight of seedling was found for all the primed seeds as compared to non-primed seeds of both cultivars. Results showed that absolute growth rate of both varieties were remarkably influenced by the imposed drought as compared to salinity treatments. Relative growth rate, net assimilation rate, seed germination index, Timson germination index were negatively affected by 150 mM NaCl concentration for the vanguard cultivar. According to results of scanning electron microscope (SEM), osmoprimed seeds increased stomatal physiology and epidermal vigor of both cultivars significantly by boosting the water potential. Response of both B. napus cultivars was found different under induced drought and salinity treatments. It has been concluded from the results that, seed priming with growth regulators such as tocopherol, ascorbic acid and salicylic acid is considered as useful technique for enhancing the germination and growth responses of B. napus under drought and salinity stresses.

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References

  • Abbasdokht H, Edalatpisheh MR (2012) The effect of priming and salinity on physiological and chemical characteristics of wheat (Triticum aestivum L.). Desert 17(2):183–192

    Google Scholar 

  • Abdolahpour M, Lotfi R (2014) Seed priming affected physiology and grain yield of chickpea under salt stress. J Biodivers Environ Sci 5(1):442–446

    Google Scholar 

  • Ahmad I, Ullah S, Nafees M (2020) Effect of osmopriming and thermopriming on amelioration of mercuric chloride stress tolerance in mungbean (Vigna radiata L.). Plant Physiol Reports 25(3):516–528

    Article  CAS  Google Scholar 

  • Al-Abdoulhadi IA, Dinar HA, Ebert G, Buttner C (2011) Effect of salinity on leaf growth, leaf injury and biomass production in date palm (Phoenix dactylifera L.) cultivars. Indian J Sci Technol 4(11):1542–1546

    Article  Google Scholar 

  • Al-Ansari F, Ksiksi T (2016) A quantitative assessment of germination parameters: the case of Crotalaria persica and Tephrosia apollinea. Open Ecol J 9(1):13–21

    Article  Google Scholar 

  • Al-Mudaris MA (1998) Notes on various parameters recording the speed of seed germination. Tropenlandwirt-journal Agric Trop Subtrop 99(2):147–154

    Google Scholar 

  • Alam M, Hayat K, Ullah I, Sajid M, Ahmad M, Basit A et al (2020) Improving okra (abelmoschus esculentus l.) growth and yield by mitigating drought through exogenous application of salicylic acid. Fres Environ Bulle 29(01):529–535

    CAS  Google Scholar 

  • Ashraf M, McNeilly T (2004) Salinity tolerance in Brassica oilseeds. CRC Crit Rev Plant Sci 23(2):157–174

    Article  CAS  Google Scholar 

  • Barampuram S, Allen G, Krasnyanski S (2014) Effect of various sterilization procedures on the in vitro germination of cotton seeds. PCTOC 118(1):179–185

    Article  CAS  Google Scholar 

  • Berhane GG, Chala AQ (2017) Plant physiological stimulation by seeds salt priming in maize (Zea mays): Prospect for salt tolerance. Afr J Biotechnol 16(5):209–223

    Article  Google Scholar 

  • Biederman LA, Harpole WS (2013) Biochar and its effects on plant productivity and nutrient cycling: a meta-analysis. Glob Change Biol Bioenergy 5(2):202–214

    Article  CAS  Google Scholar 

  • Bina F, Bostani A (2017) Effect of Salinity (NaCl) stress on germination and early seedling growth of three medicinal plant species. Adv Life Sci 4(3):77–83

    CAS  Google Scholar 

  • Chuyong GB, Acidri T (2017) Light and moisture levels affect growth and physiological parameters differently in Faidherbia albida (Delile) A. Chev. seedlings. Acta Physiol Plantarum 39(5):1–6

    Article  CAS  Google Scholar 

  • Daszkowska-Golec A, Szarejko I (2013) Open or close the gate-stomata action under the control of phytohormones in drought stress conditions. Front Plant Sci 4:138

    Article  PubMed  PubMed Central  Google Scholar 

  • El-Saidy AE, Farouk S, Abd El-Ghany HM (2011) Evaluation of different seed priming on seedling growth, yield and quality components in two sunflower (Helianthus annuus L.) cultivars. Trends Appl Sci Res 6(9):977

    Article  CAS  Google Scholar 

  • Ensikat HJ, Ditsche-Kuru P, Barthlott W, Méndez-Vilas A (2010) Scanning electron microscopy of plant surfaces: simple but sophisticated methods for preparation and examination. Microsc Sci Technol Appl Educ 1(13):248–255

    Google Scholar 

  • Ghule PL, Dahiphale VV, Jadhav JD, Palve DK (2013) Absolute growth rate, relative growth rate, net assimilation rate as influenced on dry matter weight of Bt cotton. Int Res J Agric Econ Stat 4(1):42–46

    Google Scholar 

  • Gupta S, Pandey S (2020) Enhanced salinity tolerance in the common bean (Phaseolus vulgaris) plants using twin ACC deaminase producing rhizobacterial inoculation. Rhizosphere 16:100241

    Article  Google Scholar 

  • Hasanuzzaman M, Nahar K, Anee TI, Khan MIR, Fujita M (2018) Silicon-mediated regulation of antioxidant defense and glyoxalase systems confers drought stress tolerance in Brassica napus L. South African J Bot 115:50–57

    Article  CAS  Google Scholar 

  • Jaleel CA, Gopi R, Manivannan P, Panneerselvam R (2007) Responses of antioxidant defense system of Catharanthus roseus (L.) G. Don. to paclobutrazol treatment under salinity. Acta Physiol Plantarum 29(3):205–209

    Article  Google Scholar 

  • Jin J, Niu J, Guo T, Zhou R, Sun LZ (2020) The effect of drought on physiological responses of forage plants to salt stresses depends on occurring time. Acta Physiol Plantarum 42(6):1–10

    Article  Google Scholar 

  • Khan MI, Poor P, Janda T (2022) Salicylic acid: a versatile signaling molecule in plants. J Plant Growth Regul 41(5):1887–1890

    Article  CAS  Google Scholar 

  • Kosova K, Vitamvas P, Prasil IT, Renaut J (2011) Plant proteome changes under abiotic stress-contribution of proteomics studies to understanding plant stress response. J Proteomics 74(8):1301–1322

    Article  CAS  PubMed  Google Scholar 

  • McLean EO (1983) Soil pH and lime requirement. Methods Soil Analysis: Part 2 Chem Microbiol Prop 9:199–224

    Google Scholar 

  • Mouradi M, Bouizgaren A, Farissi M, Makoudi B, Kabbadj A, Very AA et al (2016) Osmopriming improves seeds germination, growth, antioxidant responses and membrane stability during early stage of Moroccan alfalfa populations under water deficit. Chil J Agric Res 76(3):265–272

    Article  Google Scholar 

  • Nafees M, Ullah S, Ahmed I (2021) Morphological and elemental evaluation of biochar through analytical techniques and its combined effect along with plant growth promoting rhizobacteria on Vicia faba L. under induced drought stress. Microsc Res Tech 84(12):2947–2959

    Article  CAS  PubMed  Google Scholar 

  • Nafees M, Ullah S, Ahmed I (2022) Modulation of drought adversities in Vicia faba by the application of plant growth promoting rhizobacteria and biochar. Microsc Res Tech 85(5):1856–1869

    Article  CAS  PubMed  Google Scholar 

  • Nascimento WM, Huber DJ, Cantliffe DJ (2013) Carrot seed germination and ethylene production at high temperature in response to seed osmopriming. Hortic Brasileira 31:554–558

    Article  Google Scholar 

  • Nasri N, Kaddour R, Mahmoudi H, Baatour O, Bouraoui N, Lachaal M (2011) The effect of osmopriming on germination, seedling growth and phosphatase activities of lettuce under saline condition. Afr J Biotechnol 10(65):14366–14372

    Article  CAS  Google Scholar 

  • Nouman W, Siddiqui MT, Basra S, Ahmed M, Afzal I, Rehman HU (2012) Enhancement of emergence potential and stand establishment of Moringa oleifera Lam. by seed priming. Turk J Agric For 36(2):227–235

    Google Scholar 

  • Payam M, Ahmad E, Hossein AF (2010) Physiological growth indices in winter rapeseed (Brassica napus L.) cultivars as affected by drought stress at Iran. J Cereals Oilseeds 1(1):11–16

    Google Scholar 

  • Poorter H, Remkes C (1990) Leaf area ratio and net assimilation rate of 24 wild species differing in relative growth rate. Oecologia 83(4):553–559

    Article  PubMed  Google Scholar 

  • Qureshi RH, Aslam M, Akhtar J (2003) Productivity enhancement in the salt-affected lands of Joint Satiana Pilot Project area of Pakistan. J Crop Prod 7(1–2):277–297

    Article  Google Scholar 

  • Ramzan A, Hafiz IA, Ahmad T, Abbasi NA (2010) Effect of priming with potassium nitrate and dehusking on seed germination of gladiolus (Gladiolus alatus). PAKJBOT 42(1):247–258

    Google Scholar 

  • Raza MAS, Shahid AM, Saleem MF, Khan IH, Ahmad S, Ali M, Iqbal R (2017) Effects and management strategies to mitigate drought stress in oilseed rape (Brassica napus L.): a review. Zemdirbyste 104:85–94

    Article  Google Scholar 

  • Raza SH, Shafiq F, Chaudhary M, Khan I (2013) Seed invigoration with water, ascorbic and salicylic acid stimulates development and biochemical characters of okra (Ablemoschus esculentus) under normal and saline conditions. IJAB 15(3):486–492

    CAS  Google Scholar 

  • Sabagh AE, Hossain A, Barutçular C, Islam MS, Ratnasekera D, Kumar N et al (2019) Drought and salinity stress management for higher and sustainable canola (‘Brassica napus’ L.) production: A critical review. Aust J Crop Sci 13(1):88–96

    Article  Google Scholar 

  • Sadeghi H, Khazaei F, Yari L, Sheidaei S (2011) Effect of seed osmopriming on seed germination behavior and vigor of soybean (Glycine max L.). ARPN J Agricult Biolog Sci 6(1):39–43

    Google Scholar 

  • Sardoei AS, Mohammadi GA (2014) Study of salinity effect on germination of tomato (Lycopersicon esculentum L.) genotypes. Eur J Exp Biol 4(1):283–287

    CAS  Google Scholar 

  • Semida WM, Rady MM (2014) Pre-soaking in 24-epibrassinolide or salicylic acid improves seed germination, seedling growth, and anti-oxidant capacity in Phaseolus vulgaris L. grown under NaCl stress. J Hortic Sci Biotechnol 89(3):338–344

    Article  CAS  Google Scholar 

  • Shah AN, Yang G, Tanveer M, Iqbal J (2017) Leaf gas exchange, source-sink relationship, and growth response of cotton to the interactive effects of nitrogen rate and planting density. Acta Physiol Plantarum 39(5):1–10

    Article  CAS  Google Scholar 

  • Uddin S, Ullah S, Nafees M (2021) Effect of seed priming on growth and performance of Vigna radiata L. under induced drought stress. J Agric Food Res 4:100140

    CAS  Google Scholar 

  • Ullah S, Zada J, Ali S (2016) Effect of nephthyl acetic acid foliar spray on amelioration of drought stress tolerance in maize (Zea mays L.). LCSS 47(12):1542–1558

    Article  CAS  Google Scholar 

  • Umar S, Diva I, Anjum NA, Iqbal M, Ahmad I, Pereira E (2011) Potassium-induced alleviation of salinity stress in Brassica campestris L. Cent Eur J Biol 6(6):1054–1063

    CAS  Google Scholar 

  • Vijayakumari K, Jisha KC, Puthur JT (2016) GABA/BABA priming: a means for enhancing abiotic stress tolerance potential of plants with less energy investments on defence cache. Acta Physio Plant 38(9):230

    Article  Google Scholar 

  • Yasmeen A, Basra SMA, Wahid A, Nouman W, Rehman HU (2013) Exploring the potential of Moringa oleifera leaf extract (MLE) as a seed priming agent in improving wheat performance. Turkish J Bot 37(3):512–520

    CAS  Google Scholar 

  • Zamani A, Taherzadeh M (2010) Production of low molecular weight chitosan by hot dilute sulfuric acid. Bio Resour 5(3):1554–1564

    CAS  Google Scholar 

  • Zhang X, Lu G, Long W, Zou X, Li F, Nishio T (2014) Recent progress in drought and salt tolerance studies in Brassica crops. Breed Sci 64(1):60–73

    Article  PubMed  PubMed Central  Google Scholar 

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  1. Department of Botany, University of Peshawar, 25120, Peshawar, Pakistan

    Nazish Raees, Sami Ullah & Muhammad Nafees

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  1. Nazish Raees
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Correspondence to Muhammad Nafees.

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N. Raees, S. Ullah and M. Nafees declare that they have no competing interests.

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Raees, N., Ullah, S. & Nafees, M. Interactive Effect of Tocopherol, Salicylic Acid and Ascorbic Acid on Agronomic Characters of Two Genotypes of Brassica napus L. Under Induced Drought and Salinity Stresses. Gesunde Pflanzen 75, 1905–1923 (2023). https://doi.org/10.1007/s10343-022-00808-x

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