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Effects of LPDBD Plasma and Plasma Activated Water on Germination and Growth in Rapeseed (Brassica napus)

Auswirkungen von LPDBD-Plasma und plasmaaktiviertem Wasser auf die Keimung und das Wachstum von Raps (Brassica napus)

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Abstract

The study deals with the effect of low pressure dielectric barrier discharge (LPDBD) plasma and plasma activated water (PAW) produced with Ar, O2 and Air on germination and growth in rapeseed. Although H2O2 concentration showed no significant changes, α‑amylase activity (germination inducer) significantly increased in seeds due to LPDBD plasma. In addition, the activity of SOD and CAT was significantly induced in seeds of rapeseed treated with PAW. Rapeseed plants grown from the LPDBD plasma treated seeds showed significant improvements in shoot characteristics, chlorophyll synthesis, total soluble protein and sugar concentration compared to controls. Interestingly, plasma treated plants also showed no significant variations of H2O2 in tissue(s) which is supported by the biochemical and molecular evidence(s) of antioxidant enzymes. Plants exhibited a significant increase in tissue APX and CAT activities along with BnAPX and BnCAT expression(s) in roots when seeds were treated with LPDBD Air/O2 and PAW O2. This suggests that LPDBD plasma might have been involved with elevated level of reactive oxygen species, which was tightly controlled through the upregulation of APX and CAT activities and thus trigger the growth and development in rapeseed plants. These findings reveal the role and mechanisms of LPDBD technique facilitating germination and growth in rapeseed plants.

Zusammenfassung

Die Studie beschäftigt sich mit der Wirkung von Niederdruckplasma mit dielektrischer Barriereentladung (engl. low pressure dielectric barrier discharge plasma, LPDBD plasma) und plasmaaktiviertem Wasser (PAW), die mit Ar, O2 und Luft hergestellt werden, auf die Keimung und das Wachstum von Raps. Obwohl die H2O2-Konzentration keine signifikanten Veränderungen zeigte, stieg die α‑Amylase-Aktivität (Keimungsinduktor) im Saatgut durch das LPDBD-Plasma signifikant an. Darüber hinaus wurde die Aktivität von SOD und CAT in Rapssamen, die mit PAW behandelt wurden, signifikant induziert. Rapspflanzen aus dem mit LPDBD-Plasma behandelten Saatgut zeigten signifikante Verbesserungen der Sprosseigenschaften, der Chlorophyllsynthese, der Gesamtprotein- und Zuckerkonzentration im Vergleich zur Kontrolle. Interessanterweise zeigten plasmabehandelte Pflanzen auch keine signifikanten Variationen von H2O2 in Gewebe(n), was durch die biochemischen und molekularen Nachweise von antioxidativen Enzymen bestätigt wird. Pflanzen zeigten einen signifikanten Anstieg der Gewebe-APX- und CAT-Aktivitäten sowie der BnAPX- und BnCAT-Expression(en) in den Wurzeln, wenn Samen mit LPDBD Luft/O2 und PAW O2 behandelt wurden. Dies deutet darauf hin, dass LPDBD-Plasma an einem erhöhten Level an reaktiven Sauerstoffspezies beteiligt gewesen sein könnte, was durch die Hochregulierung der APX- und CAT-Aktivitäten streng kontrolliert wurde und somit das Wachstum und die Entwicklung von Rapspflanzen auslöste. Diese Ergebnisse tragen zum Verständnis der Rolle und der Mechanismen der LPDBD-Technik bei. Durch den Einsatz dieser Technik können die Keimung und das Wachstum von Rapspflanzen verbessert werden.

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Acknowledgements

We are grateful to Bioresearch Technologies, Denmark, for supplying primers.

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Authors and Affiliations

  1. Molecular Plant Physiology Laboratory, Department of Botany, University of Rajshahi, 6205, Rajshahi, Bangladesh

    Shariful Islam & Ahmad Humayan Kabir

  2. Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, 6205, Rajshahi, Bangladesh

    Farjana Binta Omar, Salek Ahmed Sajib & Abu Reza

  3. Plasma Science and Technology Laboratory, Department of Applied Physics and Electronic Engineering, University of Rajshahi, 6205, Rajshahi, Bangladesh

    Nepal Chandra Roy & Mamunur Rashid Talukder

  4. Department of Agronomy and Agricultural Extension, University of Rajshahi, 6205, Rajshahi, Bangladesh

    Mahmodol Hasan

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  1. Shariful Islam
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  2. Farjana Binta Omar
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  3. Salek Ahmed Sajib
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  4. Nepal Chandra Roy
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  5. Abu Reza
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  7. Mamunur Rashid Talukder
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  8. Ahmad Humayan Kabir
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Correspondence to Ahmad Humayan Kabir.

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Conflict of interest

S. Islam, F.B. Omar, S.A. Sajib, N.C. Roy, A. Reza, M. Hasan, M.R. Talukder and A.H. Kabir declare that they have no competing interests.

Caption Electronic Supplementary Material

Supplementary Fig. S1. a

 Schematic diagram of LPDBD plasma for rapeseed treatment with Ar/O2 and Ar/Air gases, b V-I waveform of Ar/Air LPDBD plasma measured at applied voltage 3 kV and electrode spacing 30 mm and c emitted spectrum from Air/O2 and Ar/Air LPDBD plasmas at applied voltage \(3kV\) and electrode spacing 30 mm.

Supplementary Fig. S2. a

 Schematic diagram for the generation of PAW with Ar and O2, b V-I waveform of H2O/Ar Arc measured at applied voltage 3 kV and electrode spacing 15 mm and c emitted spectrum from H2O/O2 and H2O/Ar Arc discharge plasmas at applied voltage 3 kV and electrode spacing 15 mm.

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Islam, S., Omar, F.B., Sajib, S.A. et al. Effects of LPDBD Plasma and Plasma Activated Water on Germination and Growth in Rapeseed (Brassica napus). Gesunde Pflanzen 71, 175–185 (2019). https://doi.org/10.1007/s10343-019-00463-9

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  • DOI: https://doi.org/10.1007/s10343-019-00463-9

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