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Determination of Stoma and Leaf Characteristics with Chlorophyll and Carotenoid Amounts of Some Domestic and Foreign Olive (Olea europaea L.) Varieties

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Abstract

Olive is one of the oldest fruit species cultivated. This study was carried out to determine the stomatal and leaf morphology as well as the chlorophyll and carotenoid content of 10 different (seven domestic and three foreign) olive cultivars grown in Hatay/Türkiye ecological conditions. Stoma density: 369.44 (‘Gemlik-21’)–695.55 (‘Domat’) stoma/mm2; stoma index: 1.24 (‘Girit Zeytini’)–1.85 (‘Arbequine’); Chl(a + b): 20.66 (‘Frantoio’)–34.77 (‘Girit Zeytini’) mg/g FW; number of carotenoids: 1.98 (‘Arbequine’)–4.60 (‘Frantoio’) mg/g FW; leaf area varied between 2.29 (‘Girit Zeytini’) and 6.91 (‘Frantoio’) cm2. As a result of the correlation analysis, a positive correlation was found between stoma width and stoma length (r = 0.55, p < 0.0001). Although there was a negative correlation between leaf area with stomatal density, there was a statistically significant difference (r = −0.30, p < 0.0001). Similar results were observed between Chl(a + b) and carotenoids (r = −0.25, p < 0.01). According to principal component analysis, the first five components explain 79.08% of the total variation. According to the position plot corresponding to the correlations of olive cultivars with respect the two principal components the ‘Gemlik 21’, ‘Memecik’, ‘Edincik Su’ and ‘Gemlik’ varieties are in one group, ‘Frantoio’, ‘Manzanilla’, ‘Arbequine’ varieties are in one group, while ‘Ayvalık’ and ‘Domat’ varieties are in another group, and ‘Girit Zeytini’ are separate from other varieties.

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References

  • Al-Naqeeb DAK (2020) İzmir koşullarında sulanan ve sulanmayan bazı zeytin çeşitlerinin yapraklarındaki morfolojik ve anatomik farklılıkların belirlenmesi. Master dissertation, Ankara Üniversitesi

  • Alp Ş, Çelik F, Keskin N (2016) Bazı Gül ve Kuşburnu Türlerinde (Rosa ssp.) Stoma özellikleri ve yoğunluğunun görüntü analizi yöntemi ile belirlenmesi. Harran Tarım Ve Gıda Bilimleri Dergisi 20(3):159–165. https://doi.org/10.29050/harranziraat.259107

    Article  Google Scholar 

  • Alpaslan K (2019) Düzce yöresi kestanelerinin (Castanea sativa Mill.) bazı pomolojik ve morfolojik özelliklerinin belirlenmesi. Master dissertation, Bolu Abant İzzet Baysal Üniversitesi

  • Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24(1):1–15. https://doi.org/10.1104/pp.24.1.1

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Atar F, Güney D (2021) Change of chlorophyll content depending on different pretreatment and elevation in oriental hornbeam (Carpinus orientalis Mill.) seedlings. J Bartin Fac For 23(1):236–243. https://doi.org/10.24011/barofd.790618

    Article  Google Scholar 

  • Batos B, Vilotić D, Orlović S, Miljković D (2010) Inter and intra-population variation of leaf stomatal traits of Quercus robur L. in northern Serbia. Arch Biol Sci 62(4):1125–1136. https://doi.org/10.2298/ABS1004125B

    Article  Google Scholar 

  • Bertolino LT, Caine RS, Gray JE (2019) Impact of stomatal density and morphology on water-use efficiency in a changing world. Front Plant Sci 10:225. https://doi.org/10.3389/fpls.2019.00225

    Article  PubMed  PubMed Central  Google Scholar 

  • Bongi G, Mencuccini M, Fontanazza G (1987) Photosynthesis of olive leaves: effect of light flux density, leaf age, temperature, peltates, and H2O vapor pressure deficit on gas exchange. J Am Soc Hortic Sci 112(1):143–148. https://doi.org/10.21273/JASHS.112.1.143

    Article  Google Scholar 

  • Case S (2004) Leaf stomata as bio indicators of environmental change. TIEF, vol 1

    Google Scholar 

  • Ciha AJ, Brun WA (1975) Stomatal size and frequency in soybeans. 1. Crop Sci 15(3):309–313. https://doi.org/10.2135/cropsci1975.0011183X001500030008x

    Article  Google Scholar 

  • Çimen B, Yeşiloğlu T, İncesu M, Yılmaz B, Kaçar YA (2016) Bazı turunçgil genotiplerinden tetraploid bitki elde edilmesi. Derim 33(2):175–188. https://doi.org/10.16882/derim.2016.267423

    Article  Google Scholar 

  • Edwards M, Meidner H (1978) Stomatal responses to humidity and the water potentials of epidermal and mesophyll tissue. J Exp Bot 29(3):771–780. https://doi.org/10.1093/jxb/29.3.771

    Article  Google Scholar 

  • Gözel H (2018) İslahiye ve Hassa ilçelerinde yabani zeytin (Olea europaea L. spp. oleaster) seleksiyonu. Doctoral dissertation, Kahramanmaraş Sütçü İmam University

  • Gregoriou K, Pontikis K, Vemmos S (2007) Effects of reduced irradiance on leaf morphology, photosynthetic capacity, and fruit yield in olive (Olea europaea L.). Photosynthetica 45:172–181

    Article  Google Scholar 

  • Guerfel M, Baccouri O, Boujnah D, Chaïbi W, Zarrouk M (2009) Impacts of water stress on gas exchange, water relations, chlorophyll content and leaf structure in the two main Tunisian olive (Olea europaea L.) cultivars. Sci Hortic 119(3):257–263. https://doi.org/10.1016/j.scienta.2008.08.006

    Article  CAS  Google Scholar 

  • Hakan M (2017) Ayvacık yöresinde yabani zeytin (Olea europaea L. ssp. oleaster) seleksiyonu. Doctoral dissertation, Ege University

  • Han WX, Fang JY, Reich PB, Ian Woodward F, Wang (2011) Biogeography and variability of eleven mineral elements in plant leaves across gradients of climate, soil and plant functional type in China. Ecol Lett 14(8):788–796. https://doi.org/10.1111/j.1461-0248.2011.01641.x

    Article  CAS  PubMed  Google Scholar 

  • İkinci A, Ak BE, Dikmetaş B, Hatipoglu IH (2022) Şanlıurfa ve Diyarbakır illerinde yetişen alıç (Crataegus spp.) genotiplerinin bazı meyve, yaprak ve stoma özelliklerinin belirlenmesi. Türk Tarım Ve Doğa Bilimleri Dergisi 9(4):850–859. https://doi.org/10.30910/turkjans.1051878

    Article  Google Scholar 

  • ImageJ (2022) Image processing tool (1997–2017). National Institute of Health. https://imagej.nih.gov/ij/. Accessed 1 Dec 2022

  • Kacar B, Katkat V, Ozturk S (2006) Bitki Fizyolojisi. Nobel Yayın, vol 848

    Google Scholar 

  • Kaya H, Sefer F, Şahin M, Çetin Ö, Mete N, Güloğlu U, Hakan M, Altan K, Köktürk H (2014) Zeytin genetik kaynaklarının toplanması muhafazası ve değerlendirilmesi. Proje ara sonuç raporu. Zeytincilik Araştırma Enstitüsü, İzmir, Türkiye

    Google Scholar 

  • Khaleghi E, Arzani K, Moallemi N, Barzegar M (2012) Evaluation of chlorophyll content and chlorophyll fluorescence parameters and relationships between chlorophyll a, b and chlorophyll content index under water stress in Olea europaea cv. Dezful. Int J Agric Biosyst Eng 6(8):636–639

    Google Scholar 

  • Kutbay HG, Kılınç M (1992) Bazı bitkilerdeki klorofil a ve klorofil b içeriklerinin mevsimsel değişimi. FÜ XI. Ulusal Biyoloji Kongresi. Genel Biyoloji, vol 195(202), pp 24–27

    Google Scholar 

  • Lichtenthaler HK, Wellburn AR (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans 11:591–592

  • Montoro A, López-Urrea R, Fereres E (2016) Role of stomata density in the water use of grapevines. Acta Hortic 1115:41–48. https://doi.org/10.17660/ActaHortic.2016.1115.7

    Article  Google Scholar 

  • Muradoglu F, Gundogdu M (2011) Stomata size and frequency in some walnut (Juglans regia) cultivars. Int J Agric Biol 13(6):1011–1015

    CAS  Google Scholar 

  • Odabasioglu MI, Gursoz S (2019) Leaf and stomatal characteristics of grape varieties (Vitis vinifera L.) cultivated under semi arid climate conditions. Fresenius Environ Bull 28(11A):8501–8510

    CAS  Google Scholar 

  • Öncü B (2021) Kısıntılı sulama koşullarında yetiştirilen Gemlik zeytin fidanlarının agronomik ve fizyolojik özellikleri ile yüksek sıcaklık toleranslarının belirlenmesi. Master dissertation, Bursa Uludağ University

  • Proietti P, Famiani F (2002) Diurnal and seasonal changes in photosynthetic characteristics in different olive (Olea europaea L.) cultivars. Photosynthetica 40(2):171–176. https://doi.org/10.1023/A:1021329220613

    Article  CAS  Google Scholar 

  • Retallack GJ (2001) A 300-million-year record of atmospheric carbon dioxide from fossil plant cuticles. Nature 411(6835):287–290. https://doi.org/10.1038/35077041

    Article  CAS  PubMed  Google Scholar 

  • Roselli G, Venora G (1990) Relationship between stomatal size and winter hardiness in the olive. Acta Hortic 286:89–92. https://doi.org/10.17660/ActaHortic.1990.286.15

    Article  Google Scholar 

  • Sümbül A (2022) Determination of stoma characteristics and spad values of some local and commercial grape varieties cultivated in Kayseri ecological conditions. Erzincan Univ J Sci Technol 15(2):556–565. https://doi.org/10.18185/erzifbed.1068843

    Article  Google Scholar 

  • Sun P, Wahbi S, Tsonev T, Haworth M, Liu S, Centritto M (2014) On the use of leaf spectral indices to assess water status and photosynthetic limitations in Olea europaea L. during water-stress and recovery. PLoS ONE 9(8):1–12. https://doi.org/10.1371/journal.pone.0105165

    Article  CAS  Google Scholar 

  • Tunç Y, Yılmaz KU (2022) Investigating the rooting status of some wild (delice) olive (Olea europaea L. subps. oleaster) genotypes in Hatay province’s Hassa district. Erciyes Tarım Ve Hayvan Bilimleri Dergisi 5(2):44–49. https://doi.org/10.55257/ethabd.1171708

    Article  Google Scholar 

  • UPOV TG/99/4(proj.3) (2010) UPOV (International Union for The Protection of New Varieties of Plants Geneva) Olive Criterion-18 Nov 2010. UPOV, Geneva, Switzerland

  • Wang H, Shi H, Yang R, Liu J, Yu Y (2012) Stomatal characteristics of greening plant species in response to different urban atmospheric environments in Xi’an, China. J Food Agrıc Environ 10(3–4):1524–1529. https://doi.org/10.1234/4.2012.3728

    Article  Google Scholar 

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Authors and Affiliations

  1. Hassa Station, Hatay Olive Research Institute Directorate, 31700, Hassa, Hatay, Turkey

    Yazgan Tunç

  2. Agricultural Faculty, Horticultural Department, Kahramanmaras Sutcu Imam University, 46100, Onikisubat, Kahramanmaras, Turkey

    Kadir Uğurtan Yılmaz

  3. Agricultural Faculty, Horticultural Department, Erciyes University, 38030, Melikgazi, Kayseri, Turkey

    Mehmet Yaman

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  1. Yazgan Tunç
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  2. Kadir Uğurtan Yılmaz
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Correspondence to Yazgan Tunç or Mehmet Yaman.

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Y. Tunç, K.U. Yılmaz and M. Yaman declare that they have no competing interests.

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Tunç, Y., Yılmaz, K.U. & Yaman, M. Determination of Stoma and Leaf Characteristics with Chlorophyll and Carotenoid Amounts of Some Domestic and Foreign Olive (Olea europaea L.) Varieties. Erwerbs-Obstbau 65, 1769–1778 (2023). https://doi.org/10.1007/s10341-023-00906-8

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