Abstract
One-year-old olive trees (cv. Koroneiki) were grown in plastic containers of 50 000 cm3 under full daylight and 30, 60, and 90 % shade for two years. The effects of shade on leaf morphology and anatomy, including stomatal density and chloroplast structure, net photosynthetic rate (P N), stomatal conductance (g s), and fruit yield were studied. Shade reduced leaf thickness due to the presence of only 1–2 palisade layers and reduced the length of palisade cells and spongy parenchyma. The number of thylakoids in grana as well as in stroma increased as shade increased, while the number of plastoglobuli decreased in proportion to the reduced photosynthetically active radiation (PAR). The higher the level of shade, the lower the stomatal and trichome density, leaf mass per area (ALM), g s, and P N. Shade of 30, 60, and 90 % reduced stomatal density by 7, 16, and 27 %, respectively, while the corresponding reduction in P N was 21, 35, and 67 %. In contrast, chlorophyll a+b per fresh mass, and leaf width, length, and particularly area increased under the same shade levels (by 16, 33, and 81 % in leaf area). P N reduction was due both to a decrease in PAR and to the morphological changes in leaves. The effect of shade was more severe on fruit yield per tree (32, 67, and 84 %) than on P N indicating an effect on bud differentiation and fruit set. The olive tree adapts well to shade compared with other fruit trees by a small reduction in stomatal and trichome density, palisade parenchyma, and a significant increase in leaf area.
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References
Björkman, O., Holmgren, P.: Adaptability of the photosynthetic apparatus to irradiance in ecotypes from exposed and shaded habitats.-Physiol. Plant. 16: 889–914, 1963.
Boardman, N.K.: Comparative photosynthesis of sun and shade plants.-Annu. Rev. Plant Physiol. 28: 355–377, 1977.
Bongi, G., Long, S.P.: Light dependent damage to photosynthesis in olive leaves during chilling and high temperature stress.-Plant Cell Environ. 10: 241–249, 1987.
Bongi, G., Mencuccini, M., Fontanazza, G.: Photosynthesis of olive leaves: effect of light flux density, leaf age, temperature, peltates and H2O vapor pressure deficit on gas exchange.-J. amer. Soc. hort. Sci. 112: 143–148, 1987.
Bongi, G., Palliotti, A.: Olive.-In: Schaffer, B., Andersen, P.C. (ed.): Handbook of Environmental Physiology of Fruit Crops. Vol. I. Pp. 165–187. CRC Press, Boca Raton 1994.
Chartzoulakis, K., Therios, I., Noitsakis, B.: Effects of shading on gas exchange, specific leaf weight and chlorophyll content in four kiwifruit cultivars under field conditions.-J. hort Sci. 68: 605–611, 1993.
Hampson, C.R., Azarenko, A.N., Potter, J.R.: Photosynthetic rate, flowering and yield component alteration in hazelnut in response to different light environments.-J. amer. Soc. hort. Sci. 121: 1103–1111, 1996.
Higgins, S.S., Larsen, F.E., Bendel, R.B., Radamaker, G.K., Bassman, J.H., Bidlake, W.R., Al Wir, A.: Comparative gas exchange characteristics of potted, glasshouse-grown almond, apple, fig, grape, olive peach and Asian pear.-Scientia Horticult. 52: 313–329, 1992.
Jackson, J.E.: Light interception and utilization by orchard systems.-Hort. Rev. 2: 208–267, 1980.
Jackson, J.E., Palmer, J.W.: Effects of shade on the growth and cropping of apple trees. I. Experimental details and effects on vegetative growth.-J. hort. Sci. 52: 245–252, 1977.
Kappel, F., Flore, J.A.: Effect of shade on photosynthesis, specific leaf weight, leaf chlorophyll content and morphology of young peach trees.-J. amer. Soc. hort. Sci. 108: 541–544, 1983.
Karabourniotis, G., Fasseas, C.: The dense indumentum with its polyphenol content may replace the protective role of the epidermis in some young xeromorphic leaves.-Can. J. Bot. 74: 347–351, 1996.
Karabourniotis, G., Papadopoulos, K., Papamarkou, M., Manetas, Y.: Ultraviolet-B radiation absorbing capacity of leaf hairs.-Physiol. Plant. 86: 414–418, 1992.
Karabourniotis, G., Tzobanoglou, D., Nikolopoulos, D., Liakopoulos, G.: Epicuticular phenolics over guard cells: Exploitation for in situ stomatal counting by fluorescence microscopy and combined image analysis.-Ann. Bot. 87: 631–639, 2001.
Leon, J.M., Bukovac, M.J.: Cuticle development and surface morphology of olive leaves with reference to penetration of foliar-applied chemicals.-J. amer. Soc. hort. Sci. 103: 465–472, 1978.
Liakoura, V., Stefanou, M., Manetas, Y., Cholevas, C., Karabourniotis, G.: Trichome density and its UV-B protective potential are affected by shading and leaf position on the canopy.-Environ. exp. Bot. 8: 223–229, 1997.
Marler, T.E., Schaffer, B., Crane, J.H.: Developmental light level affects growth, morphology, and leaf physiology of young carambola trees.-J. amer. Soc. hort. Sci. 119: 711–718, 1994.
Moran, R.: Formulae for determination of chlorophyllous pigments extracted with N,N-dimethylformamide.-Plant Physiol. 69: 1376–1381, 1982.
Nii, N., Kuroiwa, T.: Anatomical changes including chloroplast structure in peach leaves under different light conditions.-J. hort. Sci. 63: 37–45, 1988.
Olesinski, A.A., Wolf, S., Rudich, J., Marani, A.: Effect of leaf age and shading on photosynthesis in potatoes (Solanum tuberosum).-Ann. Bot. 64: 643–650, 1989.
Priestley, C.: The annual turnover of resources in young olive trees.-J. hort. Sci. 52: 105–112, 1977.
Proietti, P.: Effect of fruiting on leaf gas exchange in olive (Olea europaea L.).-Photosynthetica 38: 397–402, 2000.
Proietti, P., Prezios, P., Tombesi, A.: Influence of shading on olive leaf photosynthesis.-In: Proceedings of 2nd International meeting on Mediterranean Tree Crops. Pp. 334–342. Chania 1988.
Roselli, G., Benelli, G., Morelli, D.: Relationship between stomatal density and winter hardiness in olive (Olea europea L.).-J. hort. Sci. 64: 199–203, 1989.
Salisbury, F.B., Ross, C.: Photomorphogenesis. —In: Plant Physiology. 2nd Ed. Pp. 438–463. Wadsworth Publ. Co., Belmont 1978.
Savidis, T.H., Eleftheriou, P.E., Tsekos, I.: The floral nectarines of Hibiscus rosa-sinensis. III. A morphometric and ultrastructural approach.-Nordic J. Bot. 9: 63–71, 1989.
Stutte, G.W., Martin, G.C.: Effect of irradiance and carbohydrate reserves on flowering in olive.-J. amer. Soc. hort. Sci. 111: 27–31, 1986.
Syvertsen, J.P., Lloyd, J., McConchie, C., Kriedemann, P.E., Farquhar, G.D.: On the relationship between leaf anatomy and CO2 diffusion through the mesophyll of hypostomatous leaves.-Plant Cell Environ. 18: 149–157, 1995.
Syvertsen, J.P., Smith, M.L., Jr.: Light acclimation in citrus leaves. I. Changes in physical characteristics, chlorophyll and nitrogen content.-J. amer. Soc. hort. Sci. 109: 807–812, 1984.
Tombesi, A.: [Photosynthesis and interception of light energy by olive trees.]-Progetto Agricoltura (Marzo-Aprile): 36–39, 1992. [In Ital.]
Tombesi, A., Cartechini, A. [The effect of crown shading on the differentiation of flowering buds in olive.]-Riv. Ortoflorofrutticolt. ital. 70: 277–285, 1986. [In Ital.]
Tombesi, A., Colonna, P., Lancia, A. [Photosynthetic activity and chilling stress on olive C.V. Maurino.]-Atti G. Olio umbre Folingo: 1–12, 1984. [In Ital.]
Tombesi, A., Standardi, A.: [Effect of light exposure on fruitfulness on olive trees.]-Riv. Ortoflorofrutticolt. ital. 51: 368–380, 1977. [In Ital.]
Toth, R.: An introduction to morphometric cytology and its application to botanical research.-Amer. J. Bot. 69: 1694–1706, 1982.
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Gregoriou, K., Pontikis, K. & Vemmos, S. Effects of reduced irradiance on leaf morphology, photosynthetic capacity, and fruit yield in olive (Olea europaea L.). Photosynthetica 45, 172–181 (2007). https://doi.org/10.1007/s11099-007-0029-x
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DOI: https://doi.org/10.1007/s11099-007-0029-x