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Preparation of a Novel Amino-Phosphate Zwitterionic Stationary Phase for Hydrophilic Interaction Chromatography

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Abstract

A new stationary phase which contains both negatively charged phosphate groups and positively charged amino groups was successfully synthesized by modification of amino-functionalized silica particles with trichlorophosphine oxide (POCl3) for hydrophilic interaction chromatography (HILIC). The composition of the surface grafts was determined by Fourier transform infrared spectroscopy and elemental analysis. Various parameters, such as column temperature, water content, pH values and ionic strength of the mobile phase were investigated to study the retention mechanism. The results demonstrated that the stationary phase involved a complex retention process including surface adsorption, partitioning and electrostatic interactions. Under optimized conditions, the separation of nucleobases and nucleosides, water-soluble vitamins, organic acids on the novel stationary phase could be achieved in the HILIC mode.

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Acknowledgments

The authors thank the financial support from the National Key Basic Research Foundation of China (973 Program) (2013CB910702,2012CB720601), the National Natural Science Foundation of China (91017013, 31070327, 21005057), and the Fundamental Research Funds for the Central Universities.

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Authors and Affiliations

  1. Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, 430072, China

    Xiao-Dong Cheng, Xi-Tian Peng, Qiong-Wei Yu, Bi-Feng Yuan & Yu-Qi Feng

  2. Institute of Quality Standards and Testing Technology for Agro-Products, Hubei Academy of Agricultural Sciences, Wuhan, 430064, China

    Xi-Tian Peng

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  1. Xiao-Dong Cheng
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  2. Xi-Tian Peng
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  3. Qiong-Wei Yu
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  4. Bi-Feng Yuan
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Correspondence to Yu-Qi Feng.

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Cheng, XD., Peng, XT., Yu, QW. et al. Preparation of a Novel Amino-Phosphate Zwitterionic Stationary Phase for Hydrophilic Interaction Chromatography. Chromatographia 76, 1569–1576 (2013). https://doi.org/10.1007/s10337-013-2534-3

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  • DOI: https://doi.org/10.1007/s10337-013-2534-3

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