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Evaluation of the Chromatographic Performance of Conventional, Polar-Endcapped and Calixarene-Bonded Stationary Phases for the Separation of Water-Soluble Vitamins

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Abstract

The performance of four different reversed-phase columns which included a conventional C18 phase, a C18 polar-endcapped phase, an ether-linked phenyl polar-endcapped phase and a calixarene-bonded phase has been systematically compared for the separation of mixture of some water-soluble vitamins containing basic, neutral and acidic compounds of different polarities, as well as different functional groups at three pH levels and different proportions of buffer/methanol. The characteristics of water-soluble vitamins make this combination of compounds very useful as a test mixture to check column performance with real samples. Due to the physical and chemical differences between these compounds, the type of chosen column has a significant influence on the chromatographic behavior. Results of this comparison show that the C18 polar-endcapped phase was the most suitable for the separation of this group of vitamins. The presence of a polar group as an endcapping agent does not seem to influence the overall hydrophobic nature of the polar-endcapped stationary phases. At the same time, these phases displayed enhanced hydrogen bonding and silanol activity.

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Acknowledgments

C. Chamseddin has a PhD scholarship from the German Academic Exchange Service (DAAD) and the Syrian ministry of high education.

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  1. Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Ernst-Moritz-Arndt-University Greifswald, F.-L.-Jahn-Str. 17, 17487, Greifswald, Germany

    Chamseddin Chamseddin & Thomas Jira

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  1. Chamseddin Chamseddin
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  2. Thomas Jira
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Correspondence to Thomas Jira.

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Chamseddin, C., Jira, T. Evaluation of the Chromatographic Performance of Conventional, Polar-Endcapped and Calixarene-Bonded Stationary Phases for the Separation of Water-Soluble Vitamins. Chromatographia 76, 449–457 (2013). https://doi.org/10.1007/s10337-013-2417-7

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  • DOI: https://doi.org/10.1007/s10337-013-2417-7

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