Skip to main content
SpringerLink
Log in

Simple, Sensitive, High-Throughput Method for the Quantification of Mitragynine in Rat Plasma Using UPLC-MS and Its Application to an Intravenous Pharmacokinetic Study

  • Original
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

A simple, sensitive and rapid ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) method was developed and validated for the quantification of mitragynine in rat plasma using amitriptyline hydrochloride as an internal standard. Sample preparation involved a one-step liquid–liquid extraction using methyl t-butyl ether. Mitragynine was separated on an Acquity UPLC™ BEH HILIC column using isocratic elution with a mobile phase of 10 mM ammonium formate buffer containing 0.1% formic acid:acetonitrile (15:85, v/v). At a flow rate of 0.2 mL min−1, the retention time of mitragynine was found to be 1.3 min. Ionization was performed in the positive ion electrospray mode. The selected mass-to-charge (m/z) ratio transition of mitragynine ion [M + H]+ used in the selected ion recording (SIR) was 399.1. The calibration curve was found to be linear over a concentration range of 1–5,000 ng mL−1 (r = 0.999) with a lower limit of quantification (LLOQ) of 1 ng mL−1. Intra- and inter-day assay variations were found to be less than 15%. The extraction recoveries ranged from 85–93% at the three concentrations (2, 400 and 4,000 ng mL−1) in rat plasma. This method was successfully used to quantify mitragynine in rat plasma following intravenous administration of the compound.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References:

  1. Moklas M.A.M NRAR, Taufik Hidayat M, Sharida F, Farah Idayu N, Zulkhairi A, Shamima A.R (2008) Adv in Med Dent Sci 2:56–60

  2. Matsumoto K, Hatori Y, Murayama T, Tashima K, Wongseripipatana S, Misawa K, Kitajima M, Takayama H, Horie S (2006) Eur J Pharmacol 549:63–70

    Article  CAS  Google Scholar 

  3. Boyer EW, Babu KM, Adkins JE, McCurdy CR, Halpern JH (2008) Addiction 103:1048–1050

    Article  Google Scholar 

  4. Assanangkornchai S, Muekthong A, Sam-Angsri N, Pattanasattayawong U (2007) Subst Use Misuse 42:2145–2157

    Article  Google Scholar 

  5. Kikura-Hanajiri R, Kawamura M, Maruyama T, Kitajima M, Takayama H, Goda Y (2009) Forensic Toxicol 27:67–74

    Article  CAS  Google Scholar 

  6. Watanabe K, Yano S, Horie S, Yamamoto LT (1997) Life Sci 60:933–942

    Article  CAS  Google Scholar 

  7. Matsumoto K, Mizowaki M, Suchitra T, Murakami Y, Takayama H, Sakai S, Aimi N, Watanabe H (1996) Eur J Pharmacol 317:75–81

    Article  CAS  Google Scholar 

  8. Thongpradichote S, Matsumoto K, Tohda M, Takayama H, Aimi N, Sakai S, Watanabe H (1998) Life Sci 62:1371–1378

    Article  CAS  Google Scholar 

  9. Kumarnsit E, Vongvatcharanon U, Keawpradub N, Intasaro P (2007) Neurosci Lett 416:128–132

    Article  CAS  Google Scholar 

  10. Matsumoto K, Horie S, Ishikawa H, Takayama H, Aimi N, Ponglux D, Watanabe K (2004) Life Sci 74:2143–2155

    Article  CAS  Google Scholar 

  11. Suwanlert S (1975) Bull Narc 27:21–27

    CAS  Google Scholar 

  12. McWhirter L, Morris S (2010) Eur Addict Res 16:229–231

    Article  Google Scholar 

  13. Babu KM, McCurdy CR, Boyer EW (2008) Clin Toxicol (Phila) 46:146–152

    Article  CAS  Google Scholar 

  14. Farah Idayu N, Taufik Hidayat M, Moklas MA, Sharida F, Nurul Raudzah AR, Shamima AR, Apryani E (2010) Phytomedicine article in press

  15. Philipp AA, Wissenbach DK, Zoerntlein SW, Klein ON, Kanogsunthornrat J, Maurer HH (2009) J Mass Spectrom 44:1249–1261

    Article  CAS  Google Scholar 

  16. Lu S, Tran BN, Nelsen JL, Aldous KM (2009) J Chromatogr B Analyt Technol Biomed Life Sci 877:2499–2505

    Article  CAS  Google Scholar 

  17. de Moraes NV, Moretti RAC, Furr EB III, McCurdy CR, Lanchote VL (2009) J Chromatogr B 877:2593–2597

    Article  Google Scholar 

  18. Chittrakarn S, Keawpradub N, Sawangjaroen K, Kansenalak S, Janchawee B (2010) J Ethnopharmacol 129:344–349

    Article  CAS  Google Scholar 

  19. Janchawee B, Keawpradub N, Chittrakarn S, Prasettho S, Wararatananurak P, Sawangjareon K (2007) Biomed Chromatogr 21:176–183

    Article  CAS  Google Scholar 

  20. Parthasarathy S, Ramanathan S, Ismail S, Adenan MI, Mansor SM, Murugaiyah V (2010) Anal Bioanal Chem 397:2023–2030

    Article  CAS  Google Scholar 

  21. Li L, Pabbisetty D, Zhu S, Avery MA, Avery BA (2008) J Chromatogr Sci 46:215–219

    CAS  Google Scholar 

  22. Ponglux D, Wongseripipatana S, Takayama H, Kikuchi M, Kurihara M, Kitajima M, Aimi N, Sakai S (1994) Planta Med 60:580–581

    Article  CAS  Google Scholar 

  23. Takayama H, Ishikawa H, Kitajima M, Aimi N, Aji BM (2004) Chem Pharm Bull (Tokyo) 52:359–361

    Article  CAS  Google Scholar 

  24. Carvalho P, Furr EB III, McCurdy C (2009) Acta Crystallographica Section E 65:o1441–o1442

    Article  Google Scholar 

  25. Guidance for industry, bioanalytical method validation, US Department of Health and Human Services, Food and Drug Administration, May, 2001 BP. Available: http://www.fda.gov/cv

  26. Booze RM, Lehner AF, Wallace DR, Welch MA, Mactutus CF (1997) Neurotoxicol Teratol 19:7–15

    Article  CAS  Google Scholar 

  27. Cornaire G, Woodley J, Hermann P, Cloarec A, Arellano C, Houin G (2004) Int J Pharm 278:119–131

    Article  CAS  Google Scholar 

  28. Shin JH, Choi KY, Kim YC, Lee MG (2004) Antimicrob Agents Chemother 48:1756–1762

    Article  CAS  Google Scholar 

  29. Lin JH, Hooke KF, Yeh KC, Duggan DE (1985) J Pharmacol Exp Ther 235:402–406

    CAS  Google Scholar 

  30. Hewavitharana AK, Lee S, Dawson PA, Markovich D, Shaw PN (2008) Anal Biochem 374:106–111

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The project described was supported by grant number P20RR021929 from the National Center for Research Resources. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Center for Research Resources or the National Institutes of Health. This investigation was conducted in a facility constructed with support from research facilities improvement program C06 RR-14503-01 from the National Center for Research Resources.

Author information

Authors and Affiliations

  1. Department of Pharmaceutics, School of Pharmacy, The University of Mississippi, University, MS, 38677, USA

    Pradeep K. Vuppala, Sai P. Boddu & Bonnie A. Avery

  2. Department of Medicinal Chemistry, School of Pharmacy, The University of Mississippi, University, MS, 38677, USA

    Edward B. Furr & Christopher R. McCurdy

  3. Department of Pharmacology, School of Pharmacy, The University of Mississippi, University, MS, 38677, USA

    Christopher R. McCurdy

Authors
  1. Pradeep K. Vuppala
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sai P. Boddu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Edward B. Furr
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Christopher R. McCurdy
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Bonnie A. Avery
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bonnie A. Avery.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vuppala, P.K., Boddu, S.P., Furr, E.B. et al. Simple, Sensitive, High-Throughput Method for the Quantification of Mitragynine in Rat Plasma Using UPLC-MS and Its Application to an Intravenous Pharmacokinetic Study. Chromatographia 74, 703–710 (2011). https://doi.org/10.1007/s10337-011-2128-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10337-011-2128-x

Keywords

Search

Navigation