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Metabolic fingerprint of patients showing responsiveness to treatment of septic shock in intensive care unit

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Abstract

Objective

An early metabolic signature associated with the responsiveness to treatment can be useful in the better management of septic shock patients. This would help clinicians in designing personalized treatment protocols for patients showing non-responsiveness to treatment.

Methods

We analyzed the serum on Day 1 (n = 60), Day 3 (n = 47), and Day 5 (n = 26) of patients with septic shock under treatment using NMR-based metabolomics. Partial least square discriminant analysis (PLS-DA) was performed to generate the list of metabolites that can be identified as potential disease biomarkers having statistical significance (that is, metabolites that had a VIP score > 1, and p value < 0.05, False discovery rate (FDR) < 0.05).

Results

Common significant metabolites amongst the three time points were obtained that distinguished the patients being responsive (R) and non-responsive (NR) to treatments, namely 3 hydroxybutyrate, lactate, and phenylalanine which were lower, whereas glutamate and choline higher in patients showing responsiveness.

Discussion

The study gave these metabolic signatures identifying patients’ responsiveness to treatment. The results of the study will aid in the development of targeted therapy for ICU patients.

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Acknowledgements

Authors acknowledge Centre of Biomedical Research for providing the infrastructure and resources and Centre of Scientific and Industrial Research(CSIR), INDIA, for research fellowship.

Funding

This research received no external funding.

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Authors and Affiliations

  1. Centre of Biomedical Research, SGPGIMS Campus, Raebareli Road, Lucknow, 226014, India

    Swarnima Pandey, Mohd Adnan Siddiqui & Neeraj Sinha

  2. Department of Critical Care Medicine, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Rae Bareli Road, Lucknow, Uttar Pradesh, 226014, India

    Afzal Azim

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Contributions

NS, AA and SP conceptualized and designed the methodology of the study. NS, AA and SP investigated the study. NS and AA provided the resources and supervised the study. NS and AA administered the project. NS provided financial support for the project. Formal analysis using software, data curation and validation was performed by SP. Patient sample and clinical data collection by MAS and SP. Visualization and original draft preparation by SP. Final draft review and editing by SP, MAS, AA and NS. All authors approved the final submission.

Corresponding author

Correspondence to Neeraj Sinha.

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Conflict of interest

The authors have disclosed that they do not have any potential conflicts of interest.

Ethical approval

The study was approved by the Institutional Ethical Committee of Sanjay Gandhi Post Graduate Institute of Medical Sciences with IEC code:2018-170-PhD-107 and approval code: PEG/BI/37/2019 and date of approval 28.01.19.

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Informed consent was obtained from all subjects involved in the study.

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Pandey, S., Siddiqui, M.A., Azim, A. et al. Metabolic fingerprint of patients showing responsiveness to treatment of septic shock in intensive care unit. Magn Reson Mater Phy 36, 659–669 (2023). https://doi.org/10.1007/s10334-022-01049-9

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