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Antimicrobial activity of octan-3-one released from spent mushroom substrate of shiitake (Lentinula edodes) and its inhibitory effects on plant diseases

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Abstract

Volatiles released from the spent mushroom substrate (SMS) of shiitake mushrooms (Lentinula edodes) are known to be effective for suppressing the Alternaria sooty spot disease of cabbage that is caused by Alternaria brassicicola. We analyzed a volatile compound released from shiitake SMS by gas chromatography–mass spectrometry and identified octan-3-one as a major volatile compound. Octan-3-one exerted an antimicrobial activity on a wide range of fungal and bacterial plant pathogens, and it inhibited Alternaria sooty spot of cabbage and gray mold of tomato. Octan-3-one was observed to be a fungistatic compound as after its removal, spores of A. brassicicola initiated germination. Octan-3-one is already known as an important flavor compound in mushrooms and is considered to have relatively low toxicity; our findings indicate that it may thus be useful for protecting crops from fungal and bacterial diseases in closed spaces such as greenhouses, crop storage rooms, and shipping containers.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

We express our sincere gratitude to Fukuda Farm (Tottori, Japan), JA Tottori Inaba Shiitake Mushroom Substrate Center (Tottori, Japan), and Minoru Industrial Co., Ltd. (Okayama, Japan) for providing SMS. This work was supported in part by grants from the JSPS KAKENHI (JP16K18657 and JP22K05652).

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Authors and Affiliations

  1. Graduate School of Sustainability Sciences, Tottori University, 4-101 Koyama-Minami, Tottori, 680-8553, Japan

    Yu Muto

  2. The Tottori Mycological Institute, 211 Kokoge, Tottori, 689-1125, Japan

    Emi Fukushima-Sakuno

  3. Faculty of Agriculture, Tottori University, 4-101 Koyama-Minami, Tottori, 680-8553, Japan

    Atsushi Ishihara & Kumiko Osaki-Oka

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  1. Yu Muto
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Correspondence to Kumiko Osaki-Oka.

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Muto, Y., Fukushima-Sakuno, E., Ishihara, A. et al. Antimicrobial activity of octan-3-one released from spent mushroom substrate of shiitake (Lentinula edodes) and its inhibitory effects on plant diseases. J Gen Plant Pathol 89, 122–131 (2023). https://doi.org/10.1007/s10327-022-01110-4

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