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Reactions of 52 soybean germplasm accessions with Rpp3 alleles to a panel of 13 Phakopsora pachyrhizi (soybean rust) isolates from the southern United States

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Abstract

Soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. is one of the most economically important foliar diseases of soybean [Glycine max (L.) Merr.]. At least 52 soybean plant introductions (PIs) with resistance to SBR have a resistance gene at the Rpp3 locus on chromosome 6, but little is known about the degree of similarity between those alleles and the Rpp3 alleles from PI 462312 and ‘Hyuuga’, which has resistance genes at the Rpp3 and Rpp5 loci. Reactions of 52 PIs to a panel of 13 P. pachyrhizi isolates from the United States were assessed to identify accessions with reaction patterns that differed from those of PI 462312 and Hyuuga. Thirty-two PIs were similar to PI 462312 and eight more similar to Hyuuga. Twelve PIs had reactions to 2011 and 2012 isolates from Florida that differed from both PI 462312 and Hyuuga. Although the majority of the 52 accessions appear to have the same Rpp3 allele as PI 462312, at least some of the 12 PIs with different reaction patterns are likely to have unique alleles. This information will be useful in breeding rust-resistant soybean cultivars, and potentially for identifying regions of the Rpp3 locus that are involved in P. pachyrhizi pathotype recognition. None of the P. pachyrhizi isolates collected after 2012 were as virulent as 2011 and 2012 isolates from north-central Florida, demonstrating that virulence shifts in P. pachyrhizi populations are not necessarily permanent.

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Abbreviations

BSA:

Bulked segregant analysis

Chr:

Chromosome

GRIN:

Germplasm Resources Information Network

IM:

Immune (i.e., symptomless) infection type

MG:

Maturity group

PI:

Plant introduction

RB:

Reddish-brown (resistant) infection type

SBR:

Soybean rust

TAN:

Tan (susceptible) infection type

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Acknowledgements

We are grateful to the USDA Agricultural Research Service and to the United Soybean Board for partial financial support of this research (Project no. 3611-22000-019-30R). We thank the donors of P. pachyrhizi samples, including Jim Marois and G. Kelly O’Brien (North Florida Res. & Ed. Center), Clayton Hollier (Louisiana State University); Ed Sikora (Auburn University, Alabama); and Glen Hartman (USDA-ARS, Urbana, IL). We also thank Wei Wei, Leticia Lamana, Heather Lash, and Andrew D’Urso at the University of Illinois and Randy Nelson (retired) and Todd Bedford of the USDA Soybean Germplasm Collection in Urbana, IL, USA.

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  1. Donna K. Harris

    Present address: Department of Plant Sciences, Sheridan Research and Extension Center, University of Wyoming, Sheridan, WY, 82801, USA

Authors and Affiliations

  1. Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA

    Chandra Paul & David R. Walker

  2. Institute of Plant Breeding, Genetics and Genomics and Department of Crop and Soil Sciences, University of Georgia, Athens, GA, 30602, USA

    Donna K. Harris & Zenglu Li

  3. Plant Pathology and Crop Physiology Department, Louisiana State University AgCenter, Baton Rouge, LA, 70803, USA

    Patricia A. Bollich

  4. Soybean/Maize Germplasm, Pathology and Genetics Research Unit, USDA-ARS, Urbana, IL, 61801, USA

    David R. Walker

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Paul, C., Harris, D.K., Li, Z. et al. Reactions of 52 soybean germplasm accessions with Rpp3 alleles to a panel of 13 Phakopsora pachyrhizi (soybean rust) isolates from the southern United States. J Gen Plant Pathol 87, 55–70 (2021). https://doi.org/10.1007/s10327-020-00972-w

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