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Point mutation in CYP51A1 of Venturia inaequalis is associated with low sensitivity to sterol demethylation inhibitors

  • Fungal Diseases
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Abstract

In 2016, a serious outbreak of apple scab (caused by Venturia inaequalis) in Aomori Prefecture, Japan, resulted from reduced fungal sensitivity to sterol demethylation inhibitors (DMIs). In this study, the lanosterol 14α-demethylase (CYP51A1) gene of V. inaequalis isolates from Aomori Prefecture was analyzed. A nonsynonymous nucleotide polymorphism causing an amino acid substitution from tyrosine to phenylalanine at position 133 (Y133F) in CYP51A1 was closely associated with low sensitivity to DMIs. This is first report of a point mutation in CYP51A1 associated with low sensitivity to DMIs in V. inaequalis.

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References

  • Asai K, Tsuchimori N, Okonogi K, Perfect JR, Gotoh O, Yoshida Y (1999) Formation of azole-resistant Candida albicans by mutation of sterol 14-demethylase P450. Antimicrob Agents Chemother 43:1163–1169

    Article  CAS  Google Scholar 

  • Becher R, Wirsel SGR (2012) Fungal cytochrome P450 sterol 14α-demethylase (CYP51) and azole resistance in plant and human pathogens. Appl Microbiol Biotechnol 95:825–840

    Article  CAS  Google Scholar 

  • Délye C, Laigret F, Corio-Costet MF (1997) A mutation in the 14α-demethylase gene of Uncinula necator that correlates with resistance to a sterol biosynthesis inhibitor. Appl Environ Microbiol 63:2966–2970

    Article  Google Scholar 

  • Hamamoto H, Hasegawa K, Nakaune R, Young JL, Makizumi Y, Akutsu K, Hibi T (2000) Tandem repeat of a transcriptional enhancer upstream of the sterol 14α-demethylase gene (CYP51) in Penicillium digitatum. Appl Environ Microbiol 66:3421–3426

    Article  CAS  Google Scholar 

  • Hayashi K, Schoonbeek HJ, De Waard MA (2002) Expression of the ABC transporter BcatrD from Botrytis cinerea reduces sensitivity to sterol demethylation inhibitor fungicides. Pestic Biochem Physiol 73:110–121

    Article  CAS  Google Scholar 

  • Henrίquez SJL, Samiento VO, Alarcόn CP (2011) Sensitivity of Venturia inaequalis Chilean isolates to difenoconazole, fenarimol, mancozeb, and pyrimethanil. Chil J Agr Res 71:39–44

    Article  Google Scholar 

  • Hirayama K, Hanaoka T, Araya J, Tushima Y, Akahira T (2017) DMIs-resistant strains of Venturia inaequalis causing apple scab in Aomori Prefecture, Japan (in Japanese). Ann Rept Plant Prot North Japan 68:108–114

    Google Scholar 

  • Hulvey J, Popko JT, Sang H, Berg A, Jung G (2012) Overexpression of ShCYP51B and ShatrD in Sclerotinia homoeocarpa isolates exhibiting practical field resistance to a demethylation inhibitor fungicide. Appl Environ Microbiol 78:6674–6682

    Article  CAS  Google Scholar 

  • Köller W, Parker DM, Reynolds KL (1991) Baseline sensitivities of Venturia inaequalis to sterol demethylation inhibitors. Plant Dis 75:726–728

    Article  Google Scholar 

  • Ma Z, Proffer TJ, Jacobs JL, Sundin GW (2006) Overexpression of the 14α-demethylase target gene (CYP51) mediates fungicide resistance in Blumeriella jaapii. Appl Environ Microbiol 72:2581–2585

    Article  CAS  Google Scholar 

  • Nakaune R, Adachi K, Nawata O, Tomiyama M, Akutsu K, Hibi T (1998) A novel ATP-binding cassette transporter involved in multidrug resistance in the phytopathogenic fungus Penicillium digitatum. Appl Environ Microbiol 64:3983–3988

    Article  CAS  Google Scholar 

  • Schnabel G, Jones AL (2001) The 14α–demethylasse (CYP51A1) gene is overexpressed in Venturia inaequalis strains resistant to myclobutanil. Phytopathology 91:102–110

    Article  CAS  Google Scholar 

  • Stammler G, Cordero J, Koch A, Semar M, Schlehuber S (2009) Role of the Y134F mutation in cyp51 and overexpression of cyp51 in the sensitivity response of Puccinia triticina to epoxiconazole. Crop Prot 28:891–897

    Article  CAS  Google Scholar 

  • Villani SM, Hulvey J, Hily JM, Cox KD (2016) Overexpression of the CYP51A1 gene and repeated elements are associated with differential sensitivity to DMI fungicides in Venturia inaequalis. Phytopathology 106:562–571

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by grants from the Project of the NARO Bio-oriented Technology Research Advancement Institution (Research program on development of innovative technology). We thank Robert McKenzie, PhD, from Edanz Group (www.edanzediting.com/ac), for editing a draft of this manuscript.

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Authors and Affiliations

  1. Division of Apple Research, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), 92-24 Nabeyashiki, Shimokuriyagawa, Morioka, Iwate, 020-0123, Japan

    Hajime Yaegashi & Tsutae Ito

  2. Apple Experiment Station, Aomori Prefectural Agriculture and Forestry Research Center, Kuroishi, 036-0332, Japan

    Kazuyuki Hirayama & Tomoya Akahira

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  1. Hajime Yaegashi
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  2. Kazuyuki Hirayama
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  3. Tomoya Akahira
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  4. Tsutae Ito
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Correspondence to Hajime Yaegashi.

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Yaegashi, H., Hirayama, K., Akahira, T. et al. Point mutation in CYP51A1 of Venturia inaequalis is associated with low sensitivity to sterol demethylation inhibitors. J Gen Plant Pathol 86, 245–249 (2020). https://doi.org/10.1007/s10327-020-00924-4

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  • DOI: https://doi.org/10.1007/s10327-020-00924-4

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