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Histochemical detection of Blueberry latent virus in highbush blueberry plant

  • Viral and Viroid Diseases
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Abstract

Blueberry latent virus (BBLV) was detected in 27 of 95 asymptomatic highbush blueberry trees in a blueberry field in Japan. In situ hybridization showed that the viral RNAs were detected in the palisade mesophyll, spongy mesophyll and vascular bundle of leaves. In western blot analysis, an antibody to the viral protein encoded by open reading frame 1 (ORF1) reacted with a 48-kDa protein specific for blueberry trees in which BBLV was detected. Immunogold electron microscopy revealed that amorphous bodies in the cytoplasm of blueberry cells were labeled with antibodies to the ORF1 protein.

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Acknowledgments

This work was supported in part by Grant-in-Aid for Scientific Research 21580051 from the Ministry of Education, Science, Sports, and Culture of Japan.

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Authors and Affiliations

  1. Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, Morioka, 020-8550, Japan

    Masamichi Isogai, Tatuto Nakamura, Koji Ishii, Manabu Watanabe, Noriko Yamagishi & Nobuyuki Yoshikawa

Authors
  1. Masamichi Isogai
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  2. Tatuto Nakamura
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  3. Koji Ishii
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  4. Manabu Watanabe
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  5. Noriko Yamagishi
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  6. Nobuyuki Yoshikawa
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Corresponding author

Correspondence to Masamichi Isogai.

Additional information

The nucleotide sequence data reported in this paper are available in the GenBank/EMBL/DDBJ database as accessions AB608991 for BBLV isolates from Japan.

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Isogai, M., Nakamura, T., Ishii, K. et al. Histochemical detection of Blueberry latent virus in highbush blueberry plant. J Gen Plant Pathol 77, 304–306 (2011). https://doi.org/10.1007/s10327-011-0323-0

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  • DOI: https://doi.org/10.1007/s10327-011-0323-0

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