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Detection of viruses associated with rugose wood in Japanese grapevines and analysis of genomic variability of Rupestris stem pitting-associated virus

  • Viral and Viroid Diseases
  • Published:
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Abstract

In Vitis labruscana ‘Pione’ and ‘Kyoho’, 57 grapevines with rugose wood (RW) symptoms and 31 asymptomatic grapevines were obtained from vineyards in Hiroshima and Okayama prefectures. We surveyed vines for RW-associated viruses and other viruses by reverse transcription-polymerase chain reaction with virus-specific primers and degenerate primers for simultaneous detection of viruses associated with RW or leafroll disease. Rupestris stem pitting-associated virus (RSPaV) was detected from all grapevines with RW symptoms and from eight asymptomatic grapevines. Grapevine leafroll-associated virus-1, -2, -3, Grapevine virus B, Grapevine fleck virus, and Grapevine asteroid mosaic-associated virus were detected from some of the grapevines tested. RSPaV isolates detected were clustered in four groups by phylogenetic analysis using the nucleotide sequences of coat protein gene. These results suggest that RSPaV with diverse genetic variants is highly related to RW in Japanese grapevine cultivars Pione and Kyoho.

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Acknowledgments

This study was supported partially by the NARO Research Project No. 166 ‘Establishment of Agricultural Production Technologies Responding to Global Warming’ and a Grant-in-Aid for Scientific Research (No. 17780038) from the Ministry of Education, Science, and Culture of Japan. We thank Mr Satoshi Toda for discussion about phylogenetic analysis.

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Authors and Affiliations

  1. Grape and Persimmon Research Station, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization, Akitsu 301-2, Higashihiroshima, Hiroshima, 739-2494, Japan

    Ryoji Nakaune & Masaaki Nakano

  2. Agricultural Experimental Station, Okayama Prefectural General Agriculture Center, Koudaoki, Akaiwa, Okayama, 709-0801, Japan

    Koji Inoue & Hideo Nasu

  3. Research Institute of Fruit Tree, Hiroshima Prefectural Agriculture Research Center, Akitsu 2835, Higashihiroshima, Hiroshima, 739-2402, Japan

    Katsura Kakogawa & Hiromichi Nitta

  4. Citrus Research Station, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization, Kuchinotsu 954, Nagasaki, 859-2501, Japan

    Jun Imada

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  1. Ryoji Nakaune
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  2. Koji Inoue
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  3. Hideo Nasu
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  4. Katsura Kakogawa
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  5. Hiromichi Nitta
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  6. Jun Imada
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  7. Masaaki Nakano
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Correspondence to Ryoji Nakaune.

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Nakaune, R., Inoue, K., Nasu, H. et al. Detection of viruses associated with rugose wood in Japanese grapevines and analysis of genomic variability of Rupestris stem pitting-associated virus . J Gen Plant Pathol 74, 156–163 (2008). https://doi.org/10.1007/s10327-008-0079-3

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  • DOI: https://doi.org/10.1007/s10327-008-0079-3

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