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Soil microbial diversity and C turnover modified by tillage and cropping in Laos tropical grassland

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Abstract

Agricultural practices should modify the diversity of soil microbes. However, the precise relationships between soil properties and microbial diversity are poorly known. Here, we study the effect of agricultural management on soil microbial diversity and C turnover in tropical grassland of north-eastern Laos. Three years after native grassland conversion into agricultural land, we compared soils from five land use management systems: one till versus two no-till rotational cropping systems, one no-till improved pasture and the natural grassland. Soils were incubated in microcosms during 64 days at optimum temperature and humidity. Bacterial and fungal diversity were evaluated by metagenomic 454-pyrosequencing of 16S and 18SrRNA genes, respectively. Changes in soil respiration patterns were evaluated by monitoring 12C- and 13C-CO2 release after soil amendment with 13C-labelled wheat residues. Results show that residue mineralization increased with bacterial richness and diversity in the tilled treatment 7 days after soil amendment. Native soil organic C mineralization and priming effect increased with fungal richness and diversity in improved pasture and natural grassland. No-till cropping systems represented intermediate situations between tillage and pasture systems. Our findings evidence the potential of controlling soil microbial diversity by agricultural practices to improve soil biological properties. We suggest the promotion of no-till systems as a fair compromise between the need for agriculture intensification and soil ecological processes preservation.

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Acknowledgments

The authors would like to thank the Sector-Based Program in Agroecology (PROSA), the French Development Agency (AFD), the French Global Environment Facility (FFEM) and the French Ministry of Foreign Affairs for their financial support. This work, through the involvement of technical facilities of the GenoSol platform of the infrastructure ANAEE-Services, received a grant from the French state through the National Agency for Research under the programme “Investments for the Future” (reference AN-11-INBS-0001), as well as grant from the Regional Council of Burgundy.

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Authors and Affiliations

  1. CIRAD, UR SIA, 34398, Montpellier cedex 5, France

    Pascal Lienhard & Florent Tivet

  2. NAFRI, NCAC, PO Box 7170, Vientiane, Lao PDR

    Pascal Lienhard & Khamkéo Panyasiri

  3. INRA-Université Bourgogne, AgroSup Dijon, France

    Pascal Lienhard, Sébastien Terrat, Nicolas Chemidlin Prévost-Bouré, Virginie Nowak, Tiffanie Régnier, Céline Faivre, Lionel Ranjard & Pierre-Alain Maron

  4. Plateforme GenoSol, UMR1347 Agroécologie, BP 86510, 21000, Dijon, France

    Pascal Lienhard, Sébastien Terrat, Virginie Nowak, Tiffanie Régnier, Céline Faivre, Lionel Ranjard & Pierre-Alain Maron

  5. Plateforme ISOTOPE, UMR Biogeoscience, 21000, Dijon, France

    Olivier Mathieu & Jean Levêque

  6. PROSA/MAF, PO Box 10118, Vientiane, Lao PDR

    Sengphanh Sayphoummie

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  1. Pascal Lienhard
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  2. Sébastien Terrat
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  3. Olivier Mathieu
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  4. Jean Levêque
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  10. Khamkéo Panyasiri
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  11. Florent Tivet
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  13. Pierre-Alain Maron
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Correspondence to Pierre-Alain Maron.

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Lienhard, P., Terrat, S., Mathieu, O. et al. Soil microbial diversity and C turnover modified by tillage and cropping in Laos tropical grassland. Environ Chem Lett 11, 391–398 (2013). https://doi.org/10.1007/s10311-013-0420-8

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  • DOI: https://doi.org/10.1007/s10311-013-0420-8

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