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Deletion of PHO13 improves aerobic l-arabinose fermentation in engineered Saccharomyces cerevisiae

  • Bioenergy/Biofuels/Biochemicals - Short Communication
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Pentose sugars are increasingly being used in industrial applications of Saccharomyces cerevisiae. Although l-arabinose is a highlighted pentose that has been identified as next-generation biomass, arabinose fermentation has not yet undergone extensive development for industrial utilization. In this study, we integrated a heterologous fungal arabinose pathway with a deletion of PHO13 phosphatase gene. PHO13 deletion increased arabinose consumption rate and specific ethanol productivity under aerobic conditions and consequently depleted sedoheptulose by activation of the TAL1 gene. Global metabolite profiling indicated upregulation of the pentose phosphate pathway and downstream effects such as trehalose accumulation and downregulation of the TCA cycle. Our results suggest that engineering of PHO13 has ample potential for arabinose conversion to ethanol as an industrial source for biofuels.

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Fig. 1
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Abbreviations

XR:

d-xylose reductase

XDH:

Xylitol dehydrogenase

XI:

d-xylose isomerase

XK:

Xylulokinase

S. cerevisiae :

Saccharomyces cerevisiae

OD600 :

Optical density at 600 nm

RT-qPCR:

Real-Time quantitative Polymerase Chain Reaction

n.d.:

Not detected

Y Ethanol :

Ethanol yield

P Ethanol :

Specific ethanol productivity

DCW:

Dried cell weight

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Acknowledgements

This work was carried out with support from the “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ01279801)” Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. School of Food Science and Biotechnology, Kyungpook National University, Daegu, Republic of Korea

    Suji Ye, Deokyeol Jeong & Soo Rin Kim

  2. Department of Environmental Toxicology Research Center, Korea Institute of Toxicology, Jinju, Republic of Korea

    Jong Cheol Shon

  3. College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, Republic of Korea

    Jong Cheol Shon & Kwang-Hyeon Liu

  4. Department of Biotechnology, Graduate School, Korea University, Seoul, Republic of Korea

    Kyoung Heon Kim & Minhye Shin

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  1. Suji Ye
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  2. Deokyeol Jeong
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  5. Kyoung Heon Kim
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Correspondence to Minhye Shin or Soo Rin Kim.

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Ye, S., Jeong, D., Shon, J. et al. Deletion of PHO13 improves aerobic l-arabinose fermentation in engineered Saccharomyces cerevisiae. J Ind Microbiol Biotechnol 46, 1725–1731 (2019). https://doi.org/10.1007/s10295-019-02233-y

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