Abstract
The constant-rate fed-batch production of the polygalacturonic acid bioflocculant REA-11 was studied. A controlled sucrose-feeding strategy resulted in a slight improvement in biomass and a 7% reduction in flocculating activity compared with the batch process. When fed with a 3 g l−1 urea solution, the flocculating activity was enhanced to 720 U ml−1 in 36 h. High cell density (2.12 g l−1) and flocculating activity (820 U ml−1) were obtained in a 10-l fermentor by feeding with a sucrose-urea solution, with values of nearly two times and 50% higher than those of the batch process, respectively. Moreover, the residual sucrose declined to 2.4 g l−1, and residual urea decreased to 0.03 g l−1. Even higher flocculating activity of 920 U ml−1 and biomass of 3.26 g l−1 were obtained by feeding with a sucrose-urea solution in a pilot scale fermentation process, indicating the potential industrial utility of this constant-rate feeding strategy in bioflocculant production by Corynebacterium glutamicum.
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Abbreviations
- μ:
-
Specific cell growth rate
- qs :
-
Specific sucrose consumption rate
- qp :
-
Specific bioflocculant production rate
- GLC:
-
Glucose
- G6p:
-
Glucose-6-phosphate
- G1P:
-
Glucose-1-phosphate
- UDPG:
-
UDP-glucose
- UDP-GAL:
-
UDP-galactose
- UDP-GALA:
-
UDP-galacturonic acid
- polyGALA:
-
Polygalacturonic acid
- PEP:
-
Phosphoenolpyruvate
- PYR:
-
Pyruvic acid
- AcCoA:
-
AcetylCoA
- ICI:
-
Isocitric acid
- OAA:
-
Oxalacetic acid
- α-KG:
-
α-ketoglutaric acid
- SUC:
-
Succinic acid
- MAL:
-
Malic acid
- LAC:
-
Lactic acid
- AC:
-
Acetic acid
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Acknowledgments
This work was financially supported by the National Natural Science Foundation of China (no. 30700020), the Xiamen Science and Technology Committee (no. 3502Z20093006), and Fujian Provincial Department of Science and Technology, China (key project no. 2008Y0060).
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Wu, H., Li, Q., Lu, R. et al. Fed-batch production of a bioflocculant from Corynebacterium glutamicum . J Ind Microbiol Biotechnol 37, 1203–1209 (2010). https://doi.org/10.1007/s10295-010-0767-9
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DOI: https://doi.org/10.1007/s10295-010-0767-9