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Sucrose Hydrolysis in a Continuous Packed-Bed Reactor with Auto-immobilise Aspergillus niger Biocatalyst Obtained by Solid-State Fermentation

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Abstract

Invertase from Aspergillus niger C28B25 was produced by solid-state fermentation (SSF). Fermented solids were used directly as a biocatalyst for batch and continuous hydrolysis of sucrose in a packed-bed reactor under different operational conditions with various temperatures, sucrose concentrations, and feed flow rates. The SSF allowed obtaining a biocatalyst with an invertase activity of 82.2 U/g db. The biocatalyst maintained its activity in the range of 40 to 70 °C for at least 70 h of continuous operation. In a 20-mL packed bed reactor, the highest hydrolysis rate (12.3 g/g db h) was obtained at 40 °C with 2 M sucrose. Continuous hydrolysis in 20-mL and 200-mL reactors at 60 °C led to sucrose hydrolysis above 60% (8.5 residence times) and above 55% (4.5 residence times), respectively. The auto-immobilised biocatalyst produced by SSF without recovery, purification, and immobilisation stages offers an economical alternative for developing accessible biocatalysts that can be applied in batch or continuous sucrose hydrolysis processes. This study shows the potential of biocatalyst production by SSF for other enzymatic systems.

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Acknowledgements

JAMR thanks the National Council of Science and Technology (CONACyT) Mexico for the scholarship granted to carry out postgraduate studies.

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Authors and Affiliations

  1. Departamento de Biotecnologia, Universidad Autonoma Metropolitana Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, Mexico City, 09340, Mexico

    Antonio Martínez-Ruiz, Gerardo Saucedo-Castañeda & Ernesto Favela-Torres

  2. Departamento de Sistemas Biologicos, Universidad Autonoma Metropolitana Xochimilco, Calzada del Hueso 1100, Col. Villa Quietud, Delegacion Coyoacan, Mexico City, 04960, Mexico

    Luz Tovar-Castro

  3. Departamento de Investigacion en Alimentos, Facultad de Quimica, Universidad Autonoma de Coahuila, Coahuila, Saltillo, 25280, Mexico

    Cristóbal N. Aguilar

Authors
  1. Antonio Martínez-Ruiz
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  2. Luz Tovar-Castro
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  3. Cristóbal N. Aguilar
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  4. Gerardo Saucedo-Castañeda
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  5. Ernesto Favela-Torres
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Contributions

The study was conceived, done, and written by AMR and EFT. All authors: AMR, LTC, CNA, GSC, and EFT participated in the preparation of the study, discussion, critical review of the draft of the manuscript, and revision of the final version of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ernesto Favela-Torres.

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Martínez-Ruiz, A., Tovar-Castro, L., Aguilar, C.N. et al. Sucrose Hydrolysis in a Continuous Packed-Bed Reactor with Auto-immobilise Aspergillus niger Biocatalyst Obtained by Solid-State Fermentation. Appl Biochem Biotechnol 194, 1327–1339 (2022). https://doi.org/10.1007/s12010-021-03737-z

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