Abstract
An acetate negative mutant of Yarrowia lipolytica Wratislavia AWG7 was found to be suitable for the production of high amounts of citric acid in long-term repeated-batch cultures. When 40% of fresh replaced medium was fed, this strain produced 154 g l−1, on average, which corresponded to a 0.78 g g−1 yield and a productivity of 1.05 g l−1 h−1. The activity of the culture remained stable for more than 1,650 h, i.e., 16 cycles of the repeated-batch bioreactors.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anastassiadis S, Morgunov IG, Kamzolova SV, Finogenova TV (2008) Citric acid production patent review. Recent Pat Biotechnol 2(2):1–17
Anastassiadis S, Rhem HJ (2006) Citric acid production from glucose by yeast Candida oleophila ATCC 20177 under batch, continuous and repeated batch cultivation. Electron J Biotechnol 9:26–39
Arzumanov TE, Shishkanova NV, Finogenova TV (2000) Biosynthesis of citric acid by Yarrowia lipolytica repeat-batch culture on ethanol. Appl Microbiol Biotechnol 53:525–529
Crolla A, Kennedy KJ (2001) Optimization of citric acid production from Candida lipolytica Y-1095 using n-paraffin. J Biotechnol 89:27–40
Finogenova TV, Morgunov IG, Kamzolova SV, Cheryavskaya OG (2005) Organic acid production by the yeast Yarrowia lipolytica: a review of prospects. Appl Biochem Microbiol 41:418–425
Förster A, Aurich A, Mauersberger S, Barth G (2007) Citric acid production from sucrose using a recombinant strain of the yeast Yarrowia lipolytica. Appl Microbiol Biotechnol 75:1409–1417. doi:10.1007/s00253-002-1022-8
Goldberg D, Ellis G (1983) Isocitrate dehydrogenase. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Verlag Chemie, Weinheim
Imandi SB, Bandaru VVR, Somalanka SR, Garapati HR (2007) Optimization of medium constituents for the production of citric acid from byproduct glycerol using Doehlert experimental design. Enzyme Microb Technol 40:1367–1372. doi:10.1016/j.enzmictec.2006.10.012
Kamzolova SV, Morgunov IG, Aurich A, Perevoznikova OA, Shishkanova NV, Stottmeister U, Finogenova TV (2005) Lipase secretion and citric acid production in Yarrowia lipolytica yeast grown on animal and vegetable fat. Food Technol Biotechnol 43:113–122
Kautola H (1990) Itaconic acid production from xylose in repeated-batch and continuous bioreactors. Appl Microbiol Biotechnol 33(1):7–11
Levinson WE, Kurtzman CP, Kuo TM (2007) Characterization of Yarrowia lipolytica and related species for citric acid production from glycerol. Enzyme Microb Technol 41:292–295. doi:10.1016/j.enzmictec.2007.02.005
Moresi M (1994) Effect of glucose concentration on citric acid production by Yarrowia lipolytica. J Chem Technol Biotechnol 60(4):387–395
Papanikolaou S, Aggelis G (2003) Modelling aspects of the biotechnological valorization of crude glycerol: production of citric acid by Yarrowia lipolytica and 1,3-propanediol by Clostridium butyricum. J Chem Technol Biotechnol 78:542–547. doi:10.1002/jctb.831
Papanikolaou S, Fakas S, Fick M, Chevalot I, Galiotou-Panayotou M, Komaitis M, Marc I, Aggelis G (2008) Biotechnological valorisation of raw glycerol discharged after bio-diesel (fatty acid methyl esters) manufacturing process: Production of 1,3-propanediol, citric acid and single cell oil. Biomass Bioenerg 32:60–71. doi:10.1016/j.biombioe.2007.06.007
Papanikolaou S, Muniglia L, Chevalot I, Aggelis A, Marc I (2002) Yarrowia lipolytica as a potential producer of citric acid from raw glycerol. J Appl Microbiol 92:737–744
Rymowicz W, Rywińska A, Żarowska B, Juszczyk P (2006) Citric acid production from crude glycerol by acetate mutants of Yarrowia lipolytica. Chem Pap 60:391–394. doi:10.2478/s11696-006-0071-3
Rywińska A, Rymowicz W, Żarowska B, Wojtatowicz M (2009) Biosynthesis of citric acid from glycerol by acetate mutants of Yarrowia lipolytica in fed-batch fermentation. Food Technol Biotechnol 47(1):1–6
Sakurai A, Nishida Y, Saito H, Sakakibara M (2000) Ethanol production by repeated batch culture using yeast cells immobilized within porous cellulose carriers. J Biosci Bioeng 90(5):526–529. doi:10.1016/S1389-1723(01)80034-7
Stewart PR (1975) Analytical methods for yeasts. In: Prescot DM (ed) Methods in cell biology. Academic Press, New York
Soccol CR, Vandenberghe LPS, Rodrigues C, Pandey A (2006) New perspectives for citric acid production and application. Food Technol Biotechnol 44:141–149
Yu MC, Wang RC, Wang CY, Duan KJ, Sheu DC (2007) Enhanced production of l(+)-lactic acid by floc-form culture of Rhizopus oryzae. J Chin Inst Chem Eng 38(3–4):223–228
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Rywińska, A., Rymowicz, W. High-yield production of citric acid by Yarrowia lipolytica on glycerol in repeated-batch bioreactors. J Ind Microbiol Biotechnol 37, 431–435 (2010). https://doi.org/10.1007/s10295-009-0687-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10295-009-0687-8