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A new xylanase from thermoacidophilic Alicyclobacillus sp. A4 with broad-range pH activity and pH stability

  • Original Paper
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Journal of Industrial Microbiology & Biotechnology

Abstract

We have identified a highly pH-adaptable and stable xylanase (XynA4) from the thermoacidophilic Alicyclobacillus sp. A4, a strain that was isolated from a hot spring in Yunnan Province, China. The gene (xynA4) that encodes this xylanase was cloned, sequenced, and expressed in Escherichia coli. It encodes a 338-residue polypeptide with a calculated molecular mass of 42.5 kDa. The deduced amino acid sequence is most similar to (53% identity) an endo-1,4-β-xylanase from Geobacillus stearothermophilus that belongs to family 10 of the glycoside hydrolases. Purified recombinant XynA4 exhibited maximum activity at 55°C and pH 7.0, had broad pH adaptability (>40% activity at pH 3.8–9.4) and stability (retaining >80% activity after incubation at pH 2.6–12.0 for 1 h at 37°C), and was highly thermostable (retaining >90% activity after incubation at 60°C for 1 h at pH 7.0). These properties make XynA4 promising for application in the paper industry. This is the first report that describes cloning and expression of a xylanase gene from the genus Alicyclobacillus.

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Acknowledgments

This work was supported by the National High Technology Research and Development Program of China (863 Program; No. 2007AA100601) and the National Key Technology Program of China (No. 2006BAD12B05-03).

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Authors and Affiliations

  1. Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, People’s Republic of China

    Yingguo Bai, Jianshe Wang, Peilong Yang, Pengjun Shi, Huiying Luo, Kun Meng, Huoqing Huang & Bin Yao

  2. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, People’s Republic of China

    Zhifang Zhang

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  1. Yingguo Bai
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Correspondence to Bin Yao.

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Bai, Y., Wang, J., Zhang, Z. et al. A new xylanase from thermoacidophilic Alicyclobacillus sp. A4 with broad-range pH activity and pH stability. J Ind Microbiol Biotechnol 37, 187–194 (2010). https://doi.org/10.1007/s10295-009-0662-4

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  • DOI: https://doi.org/10.1007/s10295-009-0662-4

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