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Trehalose accumulation from corn starch by Saccharomycopsis fibuligera A11 during 2-l fermentation and trehalose purification

  • Original Article
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Journal of Industrial Microbiology & Biotechnology

Abstract

In this study, corn starch was used as the substrate for cell growth and trehalose accumulation by Saccharomycopsis fibuligera A11. Effect of different aeration rates, agitation speeds, and concentrations of corn starch on direct conversion of corn starch to trehalose by S. fibuligera A11 were examined using a Biostat B2 2-l fermentor. We found that the optimal conditions for direct conversion of corn starch to trehalose by this yeast strain were that agitation speed was 200 rpm, aeration rate was 4.0 l/min, concentration of corn starch was 2.0% (w/v), initial pH was 5.5, fermentation temperature was 30°C. Under these conditions, over 22.9 g of trehalose per 100 g of cell dry weight was accumulated in the yeast cells, cell mass was 15.2 g/l of the fermentation medium, 0.12% (w/v) of reducing sugar, and 0.21% (w/v) of total sugar were left in the fermented medium within 48 h of the fermentation. It was found that trehalose in the yeast cells could be efficiently extracted by the hot distilled water (80°C). After isolation and purification, the crystal trehalose was obtained from the extract of the cells.

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Acknowledgments

The authors would like to thanks Qingdao Municipal Science and Technology Commission, Qingdao, China for providing financial support to carry out this work. The grant no. is 06-2-2-22-jch.

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Authors and Affiliations

  1. Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China

    Zhe Chi, Ji-Ming Wang, Zhen-Ming Chi & Fang Ye

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  1. Zhe Chi
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  2. Ji-Ming Wang
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  3. Zhen-Ming Chi
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Correspondence to Zhen-Ming Chi.

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Chi, Z., Wang, JM., Chi, ZM. et al. Trehalose accumulation from corn starch by Saccharomycopsis fibuligera A11 during 2-l fermentation and trehalose purification. J Ind Microbiol Biotechnol 37, 19–25 (2010). https://doi.org/10.1007/s10295-009-0644-6

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