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Lignocellulolytic enzyme activity, substrate utilization, and mushroom yield by Pleurotus ostreatus cultivated on substrate containing anaerobic digester solids

  • Original Paper
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Journal of Industrial Microbiology & Biotechnology

Abstract

Solid waste from anaerobic digestion of litter from the commercial production of broiler chickens has limited use as fertilizer. Its disposal is a major problem for digester operators who are seeking alternative use for anaerobic digester solids, also referred to as solid waste (SW). The use of SW as substrates for the cultivation of Pleurotus ostreatus strain MBFBL400 was investigated. Lignocellulolytic enzymes activity, substrate utilization, and mushroom yield were evaluated in ten different substrate combinations (SCs) containing varying amounts of solid waste, wheat straw, and millet. Nutritional content of mushrooms produced on the different substrates was also determined. Substrates containing 70–80% wheat straw, 10–20% SW, and 10–20% millet were found to produce the highest mushroom yield (874.8–958.3 g/kg). Loss of organic matter in all SCs tested varied from 45.8% to 56.2%, which had positive correlation with the biological efficiency. Laccase, peroxidase, and carboxymethylcellulase (CMCase) activities were higher before fruiting, whereas xylanase showed higher activities after mushroom fruiting. SW increased the nutritional content in mushrooms harvested, and the combination of wheat straw and SW with millet significantly improved mushroom yield. Our findings demonstrated the possibility of utilizing anaerobic digester solids in mushroom cultivation. The application of SW as such could improve the financial gains in the overall economy of anaerobic digester plants.

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Acknowledgments

This research was supported by USDA grants entitled “Organic Waste Treatment Using Thermophilic Anaerobic Digestion (Bioplex) Phase 5 (grant #2005-38850-02292)” and the WVSU Gus R. Douglass Land-Grant Institute of Agricultural, Consumer, Environmental and Outreach Programs. We are grateful to Monica Haddix for help with statistical analysis, Eric Lopez for experimental work, David LeBauer and the North Carolina A&T Agricultural Research program for technical review, and to the Ag Communications team at NC A&T State University for editorial review.

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  1. Mushroom Biology and Fungal Biotechnology Laboratory, School of Agriculture and Environmental Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC, 27411, USA

    Omoanghe S. Isikhuemhen & Nona A. Mikiashvilli

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  1. Omoanghe S. Isikhuemhen
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  2. Nona A. Mikiashvilli
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Correspondence to Omoanghe S. Isikhuemhen.

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Isikhuemhen, O.S., Mikiashvilli, N.A. Lignocellulolytic enzyme activity, substrate utilization, and mushroom yield by Pleurotus ostreatus cultivated on substrate containing anaerobic digester solids. J Ind Microbiol Biotechnol 36, 1353–1362 (2009). https://doi.org/10.1007/s10295-009-0620-1

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